Protein Variants | Comment | Organism |
---|---|---|
S110P/Y295C | mutant is able to catalyze the NADH-dependent reduction of 5-hydroxymethylfurfural, an inhibitor of yeast fermentation, best activity among the mutants isolated | Saccharomyces cerevisiae |
Y295C | mutant is able to catalyze the NADH-dependent reduction of 5-hydroxymethylfurfural, an inhibitor of yeast fermentation | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxymethylfurfural | - |
Saccharomyces cerevisiae | |
acetaldehyde | - |
Saccharomyces cerevisiae | |
furfural | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
furfural | mutant S110P/Y295C, pH 6.7, 30°C | Saccharomyces cerevisiae | |
0.22 | - |
acetaldehyde | wild-type, pH 6.7, 30°C | Saccharomyces cerevisiae | |
0.83 | - |
acetaldehyde | mutant S110P/Y295C, pH 6.7, 30°C | Saccharomyces cerevisiae | |
9.45 | - |
5-hydroxymethylfurfural | mutant S110P/Y295C, pH 6.7, 30°C | Saccharomyces cerevisiae | |
18.8 | - |
furfural | wild-type, pH 6.7, 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae TMB3000 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxymethylfurfural + NADH + H+ | no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C | Saccharomyces cerevisiae | (furan-2,5-diyl)dimethanol + NAD+ | - |
? | |
5-hydroxymethylfurfural + NADH + H+ | no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C | Saccharomyces cerevisiae TMB3000 | (furan-2,5-diyl)dimethanol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ethanol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae TMB3000 | ethanol + NAD+ | - |
? | |
furfural + NADH + H+ | - |
Saccharomyces cerevisiae | furfuryl alcohol + NAD+ | - |
? | |
furfural + NADH + H+ | - |
Saccharomyces cerevisiae TMB3000 | furfuryl alcohol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH1 | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.32 | - |
furfural | mutant S110P/Y295C, pH 6.7, 30°C | Saccharomyces cerevisiae | |
12.53 | - |
acetaldehyde | wild-type, pH 6.7, 30°C | Saccharomyces cerevisiae | |
20.8 | - |
5-hydroxymethylfurfural | mutant S110P/Y295C, pH 6.7, 30°C | Saccharomyces cerevisiae | |
46 | - |
acetaldehyde | mutant S110P/Y295C, pH 6.7, 30°C | Saccharomyces cerevisiae | |
1363 | - |
furfural | wild-type, pH 6.7, 30°C | Saccharomyces cerevisiae |