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Literature summary for 1.1.1.1 extracted from

  • Laadan, B.; Wallace-Salinas, V.; Carlsson, A.J.; Almeida, J.R.; Radstroem, P.; Gorwa-Grauslund, M.F.
    Furaldehyde substrate specificity and kinetics of Saccharomyces cerevisiae alcohol dehydrogenase 1 variants (2014), Microb. Cell Fact., 13, 112.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
S110P/Y295C mutant is able to catalyze the NADH-dependent reduction of 5-hydroxymethylfurfural, an inhibitor of yeast fermentation, best activity among the mutants isolated Saccharomyces cerevisiae
Y295C mutant is able to catalyze the NADH-dependent reduction of 5-hydroxymethylfurfural, an inhibitor of yeast fermentation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxymethylfurfural
-
Saccharomyces cerevisiae
acetaldehyde
-
Saccharomyces cerevisiae
furfural
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
furfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
0.22
-
acetaldehyde wild-type, pH 6.7, 30°C Saccharomyces cerevisiae
0.83
-
acetaldehyde mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
9.45
-
5-hydroxymethylfurfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
18.8
-
furfural wild-type, pH 6.7, 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae TMB3000
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxymethylfurfural + NADH + H+ no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C Saccharomyces cerevisiae (furan-2,5-diyl)dimethanol + NAD+
-
?
5-hydroxymethylfurfural + NADH + H+ no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C Saccharomyces cerevisiae TMB3000 (furan-2,5-diyl)dimethanol + NAD+
-
?
acetaldehyde + NADH + H+
-
Saccharomyces cerevisiae ethanol + NAD+
-
?
acetaldehyde + NADH + H+
-
Saccharomyces cerevisiae TMB3000 ethanol + NAD+
-
?
furfural + NADH + H+
-
Saccharomyces cerevisiae furfuryl alcohol + NAD+
-
?
furfural + NADH + H+
-
Saccharomyces cerevisiae TMB3000 furfuryl alcohol + NAD+
-
?

Synonyms

Synonyms Comment Organism
ADH1
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.32
-
furfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
12.53
-
acetaldehyde wild-type, pH 6.7, 30°C Saccharomyces cerevisiae
20.8
-
5-hydroxymethylfurfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
46
-
acetaldehyde mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1363
-
furfural wild-type, pH 6.7, 30°C Saccharomyces cerevisiae