Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyrazole | pyrazole-sensitive enzyme forms ADH-1, ADH-2, ADH-3 and the pyrazole-insensitive form ADH-An | Coturnix coturnix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
NADH | reduction of octanal | Coturnix coturnix | |
0.011 | - |
octan-1-ol | enzyme form ADH-3 | Coturnix coturnix | |
0.013 | - |
benzyl alcohol | enzyme form ADH-2 | Coturnix coturnix | |
0.04 | - |
12-hydroxydodecanoate | enzyme form ADH-3 | Coturnix coturnix | |
0.043 | - |
octanal | enzyme form ADH-2 | Coturnix coturnix | |
0.2 | - |
pentan-1-ol | enzyme form ADH-3 | Coturnix coturnix | |
0.23 | - |
NAD+ | oxidation of ethanol | Coturnix coturnix | |
2 | - |
butan-1-ol | enzyme form ADH-3 | Coturnix coturnix | |
5.2 | - |
Cyclohexanol | enzyme form ADH-3 | Coturnix coturnix | |
8.1 | - |
ethanol | enzyme form ADH-3 | Coturnix coturnix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, enzyme form ADH-2 and ADH-3, SDS-PAGE | Coturnix coturnix |
82750 | - |
enzyme form ADH-3, equilibrium sedimentation | Coturnix coturnix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coturnix coturnix | - |
japonica. ADH-1, ADH-2, ADH-3 and ADH-An | - |
Purification (Comment) | Organism |
---|---|
ADH-2 and ADH-3 | Coturnix coturnix |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Coturnix coturnix | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-hydroxydodecanoate + NAD+ | - |
Coturnix coturnix | 12-oxododecanoic acid + NADH | - |
? | |
benzyl alcohol + NAD+ | - |
Coturnix coturnix | benzaldehyde + NADH | - |
? | |
butanol + NAD+ | - |
Coturnix coturnix | butyraldehyde + NADH | - |
? | |
cyclohexanol + NAD+ | - |
Coturnix coturnix | cyclohexanone + NADH | - |
? | |
ethanol + NAD+ | - |
Coturnix coturnix | acetaldehyde + NADH | - |
? | |
ethylene glycol + NAD+ | no activity | Coturnix coturnix | ? + NADH | - |
? | |
methanol + NAD+ | no activity | Coturnix coturnix | formaldehyde + NADH + H+ | - |
? | |
octan-1-ol + NAD+ | - |
Coturnix coturnix | n-octanal + NADH | - |
? | |
octanal + NADH + H+ | - |
Coturnix coturnix | octanol + NAD+ | - |
? | |
pentanol + NAD+ | - |
Coturnix coturnix | n-pentanal + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, enzyme form ADH-2 and ADH-3, SDS-PAGE | Coturnix coturnix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
Cyclohexanol | enzyme form ADH-2 | Coturnix coturnix | |
1.45 | - |
12-hydroxydodecanoate | enzyme form ADH-3 | Coturnix coturnix | |
1.85 | - |
octan-1-ol | enzyme form ADH-3 | Coturnix coturnix | |
2.52 | - |
benzyl alcohol | enzyme form ADH-3 | Coturnix coturnix | |
2.92 | - |
pentan-1-ol | enzyme form ADH-3 | Coturnix coturnix | |
3.55 | - |
Cyclohexanol | enzyme form ADH-3 | Coturnix coturnix | |
4.67 | - |
NAD+ | enzyme form ADH-2 | Coturnix coturnix | |
4.92 | - |
ethanol | enzyme form ADH-2 | Coturnix coturnix | |
6.18 | - |
NAD+ | enzyme form ADH-3 | Coturnix coturnix | |
6.63 | - |
ethanol | enzyme form ADH-3 | Coturnix coturnix | |
7.22 | - |
butan-1-ol | enzyme form ADH-3 | Coturnix coturnix | |
8.88 | - |
NADH | enzyme form ADH-3 | Coturnix coturnix | |
17 | - |
octanal | enzyme form ADH-3 | Coturnix coturnix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of octanal | Coturnix coturnix |
11 | - |
ethanol oxidation, enzyme form ADH-2 and ADH-3 | Coturnix coturnix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.5 | 11.5 | pH 9.5: about 40% of maximal activity, pH 11.5: about 85% of maximal activity, ethanol oxidation enzyme form ADH-3 | Coturnix coturnix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Coturnix coturnix | |
NADH | - |
Coturnix coturnix |