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Literature summary for 1.1.1.1 extracted from

  • Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.
    Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2 (2009), Microbiology, 155, 2078-2085 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 105.7% of initial activity Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus thermodenitrificans

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 18.9% of initial activity Geobacillus thermodenitrificans
Ca2+ 1 mM, 98.4% of initial activity Geobacillus thermodenitrificans
Co2+ 1 mM, 51.9% of initial activity Geobacillus thermodenitrificans
Cu2+ 1 mM, 44.2% of initial activity Geobacillus thermodenitrificans
K+ 1 mM, 70.3% of initial activity Geobacillus thermodenitrificans
Mg2+ 1 mM, 74.35% of initial activity Geobacillus thermodenitrificans
Mn2+ 1 mM, 14.3% of initial activity Geobacillus thermodenitrificans
Ni2+ 1 mM, 72.7% of initial activity Geobacillus thermodenitrificans
SDS 1 mM, 2.9% of initial activity Geobacillus thermodenitrificans
Zn2+ 1 mM, 30.5% of initial activity Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
NADP+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.4
-
NADH cosubstrate acetaldehyde, pH 8, 60°C Geobacillus thermodenitrificans
1.51
-
NAD+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
4.71
-
acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
7.55
-
ethanol pH 8, 60°C Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mM, 136.1% of initial activity Geobacillus thermodenitrificans
additional information purified enzyme does not contain a significant amount of Fe, Ca, Co, Cu, Mg, Mn or Zn Geobacillus thermodenitrificans
Na+ 1 mM, 110.5% of initial activity Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
380000
-
PAGE Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A4IP64 cf. EC 1.1.1.6 and EC 1.1.1.192
-
Geobacillus thermodenitrificans NG80-2 A4IP64 cf. EC 1.1.1.6 and EC 1.1.1.192
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, purification by nickel ion affinity chromatography Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
447.6
-
substrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + 2 NAD+ + H2O
-
Geobacillus thermodenitrificans propanedial + 2 NADH + 2 H+
-
r
acetaldehyde + NADH + H+
-
Geobacillus thermodenitrificans ethanol + NAD+
-
r
acetaldehyde + NADH + H+
-
Geobacillus thermodenitrificans NG80-2 ethanol + NAD+
-
r
butan-1-ol + NAD+
-
Geobacillus thermodenitrificans butanal + NADH + H+
-
r
decan-1-ol + NAD+
-
Geobacillus thermodenitrificans decanal + NADH + H+
-
r
dodecan-1-ol + NAD+
-
Geobacillus thermodenitrificans dodecanal + NADH + H+
-
r
eicosan-1-ol + NAD+
-
Geobacillus thermodenitrificans n-eicosanal + NADH + H+
-
r
ethanol + NAD+ ethanol is the best substrate Geobacillus thermodenitrificans acetaldehyde + NADH + H+
-
r
ethanol + NADP+
-
Geobacillus thermodenitrificans acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Geobacillus thermodenitrificans NG80-2 acetaldehyde + NADPH + H+
-
r
formaldehyde + NADH + H+
-
Geobacillus thermodenitrificans methanol + NAD+
-
r
formaldehyde + NADH + H+
-
Geobacillus thermodenitrificans NG80-2 methanol + NAD+
-
r
glycerol + NAD+
-
Geobacillus thermodenitrificans dihydroxyacetone + NADH + H+
-
r
glycerol + NAD+
-
Geobacillus thermodenitrificans NG80-2 dihydroxyacetone + NADH + H+
-
r
hexadecan-1-ol + NAD+
-
Geobacillus thermodenitrificans hexadecanal + NADH + H+
-
r
hexan-1-ol + NAD+
-
Geobacillus thermodenitrificans hexanal + NADH + H+
-
r
isoamylalcohol + NAD+
-
Geobacillus thermodenitrificans 3-methylbutanal + NADH + H+
-
r
isopropanol + NAD+
-
Geobacillus thermodenitrificans propan-2-one + NADH + H+
-
r
methanol + NAD+
-
Geobacillus thermodenitrificans formaldehyde + NADH + H+
-
r
methanol + NAD+
-
Geobacillus thermodenitrificans NG80-2 formaldehyde + NADH + H+
-
r
octacosan-1-ol + NAD+
-
Geobacillus thermodenitrificans octacosanal + NADH + H+
-
r
octadecan-1-ol + NAD+
-
Geobacillus thermodenitrificans octadecanal + NADH + H+
-
r
octan-1-ol + NAD+
-
Geobacillus thermodenitrificans octanal + NADH + H+
-
r
tetracosanol-1-ol + NAD+
-
Geobacillus thermodenitrificans tetracosanal + NADH + H+
-
r
tetradecan-1-ol + NAD+
-
Geobacillus thermodenitrificans tetradecanal + NADH + H+
-
r
triacontan-1-ol + NAD+
-
Geobacillus thermodenitrificans triacontanal + NADH + H+
-
r

Subunits

Subunits Comment Organism
octamer 8 * 45000, SDS-PAGE, 8 * 41664, calculated from sequence Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
ADH1
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus thermodenitrificans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75
-
Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
14 h, more than 50% of initial activity Geobacillus thermodenitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
43.9
-
NADP+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
404.8
-
acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
443.1
-
NAD+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
638.7
-
ethanol pH 8, 60°C Geobacillus thermodenitrificans
1645.7
-
NADH cosubstrate acetaldehyde, pH 8, 60°C Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus thermodenitrificans

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is about 2fold preferred over NADP+ Geobacillus thermodenitrificans
NADH
-
Geobacillus thermodenitrificans
NADP+ NAD+ is about 2fold preferred over NADP+ Geobacillus thermodenitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
84.6
-
ethanol pH 8, 60°C Geobacillus thermodenitrificans
85.9
-
acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
156.9
-
NADP+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
293.4
-
NAD+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1175.5
-
NADH cosubstrate acetaldehyde, pH 8, 60°C Geobacillus thermodenitrificans