General Stability | Organism |
---|---|
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde shows enhanced thermal stability and good durability in the repeated use after recovered by magnetic separations. Within 7 cycles of usage, the remaining activity is about 100%, 89.15%, 79.42%, 69.50%, 62.80%, 56.48%, and 48.26% of the first use | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.45 | - |
phenylglyoxylic acid | soluble enzyme | Saccharomyces cerevisiae | |
37.55 | - |
phenylglyoxylic acid | enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylglyoxylic acid + NADH + H+ | enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde retains 48.77% activity of its original activity | Saccharomyces cerevisiae | hydroxy(phenyl)acetic acid + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCAD | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
soluble enzyme | Saccharomyces cerevisiae |
40 | - |
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, about 35% loss of activity, soluble enzyme and enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde | Saccharomyces cerevisiae |
65 | - |
60 min, about 80% loss of activity of soluble enzyme, about 55% loss of activity of enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
soluble enzyme | Saccharomyces cerevisiae |
7.5 | - |
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde | Saccharomyces cerevisiae |