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Literature summary for 1.1.1.1 extracted from

  • Wu, X.; Zhang, C.; Orita, I.; Imanaka, T.; Fukui, T.; Xing, X.H.
    Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols (2013), Appl. Environ. Microbiol., 79, 2209-2217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
NADP+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
0.089
-
NAD+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
0.105
-
NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
0.113
-
NADP+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
0.113
-
NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
0.127
-
NAD+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
30.2
-
acetoin pH 6.0, 70°C Thermococcus kodakarensis
61.3
-
2,3-Butanediol pH 9.0, 70°C Thermococcus kodakarensis
244
-
1-phenylethanol pH 9.0, 70°C Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Thermococcus kodakarensis
31000
-
1 * 31000, SDS-PAGE, 1 * 31464, calculated Thermococcus kodakarensis
31464
-
1 * 31000, SDS-PAGE, 1 * 31464, calculated Thermococcus kodakarensis

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 20%, about 110% of initial activity Thermococcus kodakarensis
hexane 50%, about 95% of initial activity Thermococcus kodakarensis
additional information enzyme shows high resistance to organic solvents Thermococcus kodakarensis
octane 50%, about 90% of initial activity Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JI09
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(3-bromophenyl)ethanol + NAD+ 315% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(3-bromophenyl)ethanone + NADH + H+
-
?
1-(3-chlorophenyl)ethanol + NAD+ 205% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(3-chlorophenyl)ethanone + NADH + H+
-
?
1-(4-bromophenyl)ethanol + NAD+ 167% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-bromophenyl)ethanone + NADH + H+
-
?
1-(4-chlorophenyl)ethanol + NAD+ 151% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-chlorophenyl)ethanone + NADH + H+
-
?
1-(4-fluorophenyl)ethanol + NAD+ 119% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-fluorophenyl)ethanone + NADH + H+
-
?
1-(4-methylphenyl)ethanol + NAD+ 189% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-methylphenyl)ethanone + NADH + H+
-
?
1-phenyl-2-propanol + NAD+ 39% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-phenyl-2-propanone + NADH + H+
-
?
1-phenylethanol + NAD+
-
Thermococcus kodakarensis 1-phenylethanone + NADH + H+
-
?
1-phenylethanol + NADP+
-
Thermococcus kodakarensis 1-phenylethanone + NADPH + H+
-
?
2,2,2-trifluoroacetophenone + NADPH + H+ 180% of the activity with acetoin Thermococcus kodakarensis (R)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
r
2,3-butanediol + NAD+ 272% of the activity with 1-phenylethanol Thermococcus kodakarensis acetoin + NADH + H+
-
r
2-heptanol + NAD+ 62% of the activity with 1-phenylethanol Thermococcus kodakarensis heptan-2-one + NADH + H+
-
?
2-oxopropanal + NADH + H+ 328% of the activity with acetoin Thermococcus kodakarensis ? + NAD+
-
r
acetoin + NADH + H+
-
Thermococcus kodakarensis 2,3-butanediol + NAD+
-
r
cyclohexanol + NAD+ 52% of the activity with 1-phenylethanol Thermococcus kodakarensis cyclohexanone + NADH + H+
-
?
ethyl 2-oxopropanoate + NADPH + H+ 294& of the activity with acetoin Thermococcus kodakarensis (R)-ethyl 2-hydroxypropanoate + NADP+
-
r
additional information enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess Thermococcus kodakarensis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE, 1 * 31464, calculated Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
TK0845
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
half-life 4.5 h Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.4
-
NAD+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
10.5
-
NADP+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
13.7
-
acetoin pH 6.0, 70°C Thermococcus kodakarensis
15.6
-
NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
17.3
-
NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
30
-
NADP+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
30.8
-
NAD+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
35.7
-
1-phenylethanol pH 9.0, 70°C Thermococcus kodakarensis
43.8
-
2,3-Butanediol pH 9.0, 70°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
reduction of aldehydes Thermococcus kodakarensis
9
-
oxidation reaction of alcohols Thermococcus kodakarensis

pH Range

pH Minimum pH Maximum Comment Organism
6
-
50% of maximum activity for reduction of aldehydes Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermococcus kodakarensis
NADP+
-
Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
1-phenylethanol pH 9.0, 70°C Thermococcus kodakarensis
0.45
-
acetoin pH 6.0, 70°C Thermococcus kodakarensis
0.71
-
2,3-Butanediol pH 9.0, 70°C Thermococcus kodakarensis
117
-
NAD+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
149
-
NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
153
-
NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
159
-
NADP+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
243
-
NAD+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
265
-
NADP+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis