Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
NADP+ | cosubstrate 1-phenylethanol, pH 9.0, 70°C | Thermococcus kodakarensis | |
0.089 | - |
NAD+ | cosubstrate 1-phenylethanol, pH 9.0, 70°C | Thermococcus kodakarensis | |
0.105 | - |
NADH | cosubstrate acetoin, pH 6.0, 70°C | Thermococcus kodakarensis | |
0.113 | - |
NADP+ | cosubstrate 2,3-butanediol, pH 9.0, 70°C | Thermococcus kodakarensis | |
0.113 | - |
NADH | cosubstrate acetoin, pH 6.0, 70°C | Thermococcus kodakarensis | |
0.127 | - |
NAD+ | cosubstrate 2,3-butanediol, pH 9.0, 70°C | Thermococcus kodakarensis | |
30.2 | - |
acetoin | pH 6.0, 70°C | Thermococcus kodakarensis | |
61.3 | - |
2,3-Butanediol | pH 9.0, 70°C | Thermococcus kodakarensis | |
244 | - |
1-phenylethanol | pH 9.0, 70°C | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Thermococcus kodakarensis |
31000 | - |
1 * 31000, SDS-PAGE, 1 * 31464, calculated | Thermococcus kodakarensis |
31464 | - |
1 * 31000, SDS-PAGE, 1 * 31464, calculated | Thermococcus kodakarensis |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 20%, about 110% of initial activity | Thermococcus kodakarensis |
hexane | 50%, about 95% of initial activity | Thermococcus kodakarensis |
additional information | enzyme shows high resistance to organic solvents | Thermococcus kodakarensis |
octane | 50%, about 90% of initial activity | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JI09 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(3-bromophenyl)ethanol + NAD+ | 315% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-(3-bromophenyl)ethanone + NADH + H+ | - |
? | |
1-(3-chlorophenyl)ethanol + NAD+ | 205% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-(3-chlorophenyl)ethanone + NADH + H+ | - |
? | |
1-(4-bromophenyl)ethanol + NAD+ | 167% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-(4-bromophenyl)ethanone + NADH + H+ | - |
? | |
1-(4-chlorophenyl)ethanol + NAD+ | 151% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-(4-chlorophenyl)ethanone + NADH + H+ | - |
? | |
1-(4-fluorophenyl)ethanol + NAD+ | 119% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-(4-fluorophenyl)ethanone + NADH + H+ | - |
? | |
1-(4-methylphenyl)ethanol + NAD+ | 189% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-(4-methylphenyl)ethanone + NADH + H+ | - |
? | |
1-phenyl-2-propanol + NAD+ | 39% of the activity with 1-phenylethanol | Thermococcus kodakarensis | 1-phenyl-2-propanone + NADH + H+ | - |
? | |
1-phenylethanol + NAD+ | - |
Thermococcus kodakarensis | 1-phenylethanone + NADH + H+ | - |
? | |
1-phenylethanol + NADP+ | - |
Thermococcus kodakarensis | 1-phenylethanone + NADPH + H+ | - |
? | |
2,2,2-trifluoroacetophenone + NADPH + H+ | 180% of the activity with acetoin | Thermococcus kodakarensis | (R)-2,2,2-trifluoro-1-phenylethanol + NADP+ | - |
r | |
2,3-butanediol + NAD+ | 272% of the activity with 1-phenylethanol | Thermococcus kodakarensis | acetoin + NADH + H+ | - |
r | |
2-heptanol + NAD+ | 62% of the activity with 1-phenylethanol | Thermococcus kodakarensis | heptan-2-one + NADH + H+ | - |
? | |
2-oxopropanal + NADH + H+ | 328% of the activity with acetoin | Thermococcus kodakarensis | ? + NAD+ | - |
r | |
acetoin + NADH + H+ | - |
Thermococcus kodakarensis | 2,3-butanediol + NAD+ | - |
r | |
cyclohexanol + NAD+ | 52% of the activity with 1-phenylethanol | Thermococcus kodakarensis | cyclohexanone + NADH + H+ | - |
? | |
ethyl 2-oxopropanoate + NADPH + H+ | 294& of the activity with acetoin | Thermococcus kodakarensis | (R)-ethyl 2-hydroxypropanoate + NADP+ | - |
r | |
additional information | enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess | Thermococcus kodakarensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE, 1 * 31464, calculated | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
TK0845 | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life 4.5 h | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
NAD+ | cosubstrate 1-phenylethanol, pH 9.0, 70°C | Thermococcus kodakarensis | |
10.5 | - |
NADP+ | cosubstrate 1-phenylethanol, pH 9.0, 70°C | Thermococcus kodakarensis | |
13.7 | - |
acetoin | pH 6.0, 70°C | Thermococcus kodakarensis | |
15.6 | - |
NADH | cosubstrate acetoin, pH 6.0, 70°C | Thermococcus kodakarensis | |
17.3 | - |
NADH | cosubstrate acetoin, pH 6.0, 70°C | Thermococcus kodakarensis | |
30 | - |
NADP+ | cosubstrate 2,3-butanediol, pH 9.0, 70°C | Thermococcus kodakarensis | |
30.8 | - |
NAD+ | cosubstrate 2,3-butanediol, pH 9.0, 70°C | Thermococcus kodakarensis | |
35.7 | - |
1-phenylethanol | pH 9.0, 70°C | Thermococcus kodakarensis | |
43.8 | - |
2,3-Butanediol | pH 9.0, 70°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
reduction of aldehydes | Thermococcus kodakarensis |
9 | - |
oxidation reaction of alcohols | Thermococcus kodakarensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
50% of maximum activity for reduction of aldehydes | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermococcus kodakarensis | |
NADP+ | - |
Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
1-phenylethanol | pH 9.0, 70°C | Thermococcus kodakarensis | |
0.45 | - |
acetoin | pH 6.0, 70°C | Thermococcus kodakarensis | |
0.71 | - |
2,3-Butanediol | pH 9.0, 70°C | Thermococcus kodakarensis | |
117 | - |
NAD+ | cosubstrate 1-phenylethanol, pH 9.0, 70°C | Thermococcus kodakarensis | |
149 | - |
NADH | cosubstrate acetoin, pH 6.0, 70°C | Thermococcus kodakarensis | |
153 | - |
NADH | cosubstrate acetoin, pH 6.0, 70°C | Thermococcus kodakarensis | |
159 | - |
NADP+ | cosubstrate 1-phenylethanol, pH 9.0, 70°C | Thermococcus kodakarensis | |
243 | - |
NAD+ | cosubstrate 2,3-butanediol, pH 9.0, 70°C | Thermococcus kodakarensis | |
265 | - |
NADP+ | cosubstrate 2,3-butanediol, pH 9.0, 70°C | Thermococcus kodakarensis |