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Literature summary for 1.1.1.1 extracted from

  • Beer, B.; Pick, A.; Doering, M.; Lommes, P.; Sieber, V.
    Substrate scope of a dehydrogenase from Sphingomonas species A1 and its potential application in the synthesis of rare sugars and sugar derivatives (2018), Microb. Biotechnol., 11, 747-758 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingomonas sp. A1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
 D-talonate at pH 7.9 and 25°C Sphingomonas sp. A1
12
-
 D-gluconate at pH 7.9 and 25°C Sphingomonas sp. A1
13
-
 2-dehydro-3-deoxy-D-gluconate at pH 7.9 and 25°C Sphingomonas sp. A1
14
-
 D-mannonate at pH 7.9 and 25°C Sphingomonas sp. A1
16
-
 D-ribonate at pH 7.9 and 25°C Sphingomonas sp. A1
16
-
 D-allonate at pH 7.9 and 25°C Sphingomonas sp. A1
24
-
 D-xylonate at pH 7.9 and 25°C Sphingomonas sp. A1
24
-
 D-altronate at pH 7.9 and 25°C Sphingomonas sp. A1
220
-
 D-arabitol at pH 7.9 and 25°C Sphingomonas sp. A1
350
-
 D-sorbitol at pH 7.9 and 25°C Sphingomonas sp. A1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate + NADH + H+ Sphingomonas sp. A1
-
 4-deoxy-L-erythro-5-hexoseulose + NAD+
-
 r
4-deoxy-L-erythro-5-hexoseulose + NAD+ Sphingomonas sp. A1 preferred reaction 2-dehydro-3-deoxy-D-gluconate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. A1 A0A075B5H4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate + NADH + H+
-
 Sphingomonas sp. A1 4-deoxy-L-erythro-5-hexoseulose + NAD+
-
 r
4-deoxy-L-erythro-5-hexoseulose + NAD+ 100% activity Sphingomonas sp. A1 2-dehydro-3-deoxy-D-gluconate + NADH + H+
-
 r
4-deoxy-L-erythro-5-hexoseulose + NAD+ preferred reaction Sphingomonas sp. A1 2-dehydro-3-deoxy-D-gluconate + NADH + H+
-
 r
D-allonate + NADH + H+ 10% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
D-altronate + NADH + H+ 8% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
D-arabitol + NADH + H+
-
 Sphingomonas sp. A1 D-lyxose + D-arabinose + NAD+
-
 ?
D-erythrose + NAD+
-
 Sphingomonas sp. A1 ?
-
 ?
D-gluconate + NADH + H+ 79% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
D-glyceraldehyde + NAD+
-
 Sphingomonas sp. A1 ?
-
 ?
D-mannonate + NADH + H+ 37% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
D-ribonate + NADH + H+ 0.3% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
D-sorbitol + NADH + H+ 0.01% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 L-gulose + NAD+
-
 ?
D-talonate + NADH + H+ 3% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
D-threose + NAD+
-
 Sphingomonas sp. A1 ?
-
 ?
D-xylonate + NADH + H+ 1% activity compared to 2-dehydro-3-deoxy-D-gluconate Sphingomonas sp. A1 ?
-
 ?
L-glyceraldehyde + NAD+
-
 Sphingomonas sp. A1 ?
-
 ?
additional information almost no activity with D-arabinonate, D-lyxonate, D-galactonate, glycerol, meso-erythritol, D-ribitol, D-arabitol, D-xylitol, and D-mannitol. No activity with propanal, butanal, hexanal, and 4-oxobutanoic acid Sphingomonas sp. A1 ?
-
 ?

Synonyms

Synonyms Comment Organism
ADH
-
 Sphingomonas sp. A1
NAD+-dependent SDR
-
 Sphingomonas sp. A1
short-chain dehydrogenase/reductase
-
 Sphingomonas sp. A1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 the half-life of the enzyme is 20 h at 25°C in ammonium bicarbonate buffer pH 7.9 with 1 mM NAD+ Sphingomonas sp. A1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0019
-
 D-arabitol at pH 7.9 and 25°C Sphingomonas sp. A1
0.0052
-
 D-sorbitol at pH 7.9 and 25°C Sphingomonas sp. A1
0.0052
-
 D-ribonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.021
-
 D-talonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.032
-
 D-xylonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.18
-
 D-allonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.19
-
 D-altronate at pH 7.9 and 25°C Sphingomonas sp. A1
0.53
-
 D-mannonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.98
-
 D-gluconate at pH 7.9 and 25°C Sphingomonas sp. A1
1.4
-
 2-dehydro-3-deoxy-D-gluconate at pH 7.9 and 25°C Sphingomonas sp. A1

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Sphingomonas sp. A1

General Information

General Information Comment Organism
metabolism the enzyme is involved in alginate metabolism Sphingomonas sp. A1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000015
-
 D-sorbitol at pH 7.9 and 25°C Sphingomonas sp. A1
0.000087
-
 D-arabitol at pH 7.9 and 25°C Sphingomonas sp. A1
0.00033
-
 D-ribonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.0014
-
 D-xylonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.0034
-
 D-talonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.008
-
 D-altronate at pH 7.9 and 25°C Sphingomonas sp. A1
0.011
-
 D-allonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.038
-
 D-mannonate at pH 7.9 and 25°C Sphingomonas sp. A1
0.082
-
 D-gluconate at pH 7.9 and 25°C Sphingomonas sp. A1
0.1
-
 2-dehydro-3-deoxy-D-gluconate at pH 7.9 and 25°C Sphingomonas sp. A1