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Literature summary for 1.1.1.1 extracted from
- Characterization of three isoenzymes of rat alcohol dehydrogenase. Tissue distribution and physical and enzymatic properties (1987), Eur. J. Biochem., 162, 179-189.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-methoxypyrazole | 0.1-10 mM, ADH-2 is practically insensitive, ADH-3 is very sensitive | Rattus norvegicus |  |
| pyrazole | 0.1-10 mM, ADH-2 is practically insensitive, ADH-3 is very sensitive | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
NADH | isoenzyme ADH-2, pH 7.5 | Rattus norvegicus | |
| 0.013 | - |
12-hydroxydodecanoate | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 0.025 | - |
1-Octanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 0.04 | - |
NAD+ | isoenzyme ADH-2, pH 10.0 and pH 7.5 | Rattus norvegicus | |
| 0.05 | - |
benzyl alcohol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 0.05 | - |
NAD+ | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 0.08 | - |
1-Pentanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 0.1 | - |
NAD+ | isoenzyme ADH-3 | Rattus norvegicus | |
| 0.1 | - |
1-Octanol | isoenzyme ADH-2 | Rattus norvegicus | |
| 0.1 | - |
12-hydroxydodecanoate | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 0.17 | - |
1-butanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 0.2 | - |
NAD+ | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 0.25 | - |
NAD+ | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 0.3 | - |
octanal | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 0.35 | - |
2-Buten-1-ol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 0.5 | - |
1-Octanol | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 0.51 | - |
1-Octanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.4 | - |
m-nitrobenzaldehyde | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.4 | - |
benzyl alcohol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.4 | - |
12-hydroxydodecanoate | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.4 | - |
ethanol | isoenzyme ADH-3, pH 10.0 and pH 7.5 | Rattus norvegicus | |
| 1.6 | - |
Octanol | isoenzyme ADH-2, pH 7.5 | Rattus norvegicus | |
| 2.2 | - |
Cyclohexanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 2.5 | - |
2-Buten-1-ol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 3 | - |
Butanal | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 3.1 | - |
1-Pentanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 3.5 | - |
octanal | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 17 | - |
1-butanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 60 | - |
2-Buten-1-ol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 78 | - |
1-Pentanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 220 | - |
Cyclohexanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 230 | - |
1-butanol | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 340 | - |
ethanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1900 | - |
Cyclohexanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 5000 | - |
ethanol | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | ADH-1 contains 3.9 mol of zinc per mol of subunit, ADH-2 contains 4.2 mol of zinc per mol of subunit | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
2 * 39000, ADH-2, SDS-PAGE | Rattus norvegicus |
| 40000 | - |
2 * 40000, ADH-3, SDS-PAGE | Rattus norvegicus |
| 43000 | - |
2 * 43000, ADH-1, SDS-PAGE | Rattus norvegicus |
| 80000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ADH-1, ADH-2 and ADH-3 | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| cornea | - |
Rattus norvegicus | - |
| epididymis | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| intestine | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
| ovary | - |
Rattus norvegicus | - |
| pancreas | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| stomach | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
| thymus | - |
Rattus norvegicus | - |
| uterus | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
isoenzyme 4 | Rattus norvegicus |
| 0.12 | - |
isoenzyme 1 | Rattus norvegicus |
| 0.2 | - |
isoenzyme 3 | Rattus norvegicus |
| 0.62 | - |
isoenzyme 2 | Rattus norvegicus |
| 1.14 | - |
ADH-2 | Rattus norvegicus |
| 1.3 | - |
ADH-3 | Rattus norvegicus |
| 32.5 | - |
ADH-1 | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-hydroxydodecanoate + NAD+ | - |
Rattus norvegicus | 12-oxododecanoic acid + NADH | - |
? | |
| 2-butene-1-ol + NAD+ | - |
Rattus norvegicus | ? + NADH | - |
? | |
| 3-oxo-5beta-androstan-17beta-ol + NADH | - |
Rattus norvegicus | 3beta,17beta-dihydroxy-5beta-androstane + NAD+ | - |
? | |
| acetaldehyde + NADH + H+ | - |
Rattus norvegicus | ethanol + NAD+ | - |
? | |
| benzyl alcohol + NAD+ | oxidation with isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2 | Rattus norvegicus | benzaldehyde + NADH | - |
? | |
| butanol + NAD+ | pH 10.0: oxidized by ADH-1 and ADH-3, no activity with isoenzyme ADH-2 | Rattus norvegicus | butyraldehyde + NADH | - |
? | |
| cyclohexanol + NAD+ | - |
Rattus norvegicus | cyclohexanone + NADH | - |
? | |
| ethanol + NAD+ | isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2 | Rattus norvegicus | acetaldehyde + NADH | - |
? | |
| m-nitrobenzaldehyde + NADH + H+ | - |
Rattus norvegicus | m-nitrobenzyl alcohol + NAD+ | - |
? | |
| methanol + NAD+ | oxidized with ADH-3, no activity with ADH-1 and ADH-2 | Rattus norvegicus | formaldehyde + NADH + H+ | - |
? | |
| octan-1-ol + NAD+ | - |
Rattus norvegicus | n-octanal + NADH | - |
? | |
| octanal + NADH + H+ | - |
Rattus norvegicus | octanol + NAD+ | - |
? | |
| pentanol + NAD+ | - |
Rattus norvegicus | n-pentanal + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, ADH-1, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 40000, ADH-3, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 39000, ADH-2, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.383 | - |
Cyclohexanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 0.833 | - |
1-butanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1 | - |
ethanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1 | - |
benzyl alcohol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1 | - |
1-Octanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.17 | - |
1-Pentanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.27 | - |
12-hydroxydodecanoate | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.5 | - |
Cyclohexanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.67 | - |
2-Buten-1-ol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.75 | - |
Cyclohexanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 2.33 | - |
1-Octanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 3.53 | - |
1-Pentanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 3.83 | - |
12-hydroxydodecanoate | isoenzyme ADH-12, pH 10.0 | Rattus norvegicus | |
| 5.83 | - |
2-Buten-1-ol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 20 | - |
12-hydroxydodecanoate | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 48.8 | - |
1-butanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 48.8 | - |
1-Pentanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 60.8 | - |
1-Octanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 62.7 | - |
ethanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 89.7 | - |
benzyl alcohol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 213 | - |
2-Buten-1-ol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.7 | - |
oxidation of ethanol, isoenzyme 2, 3 and 4 | Rattus norvegicus |
| 11 | - |
oxidation of octanol | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
| NADH | - |
Rattus norvegicus | |
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