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Literature summary for 1.1.1.1 extracted from
- Alcohol dehydrogenases from Kluyveromyces marxianus: heterologous expression in Escherichia coli and biochemical characterization (2014), BMC Biotechnol., 14, 45.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Kluyveromyces marxianus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
acetaldehyde | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus |  |
| 4 | - |
acetaldehyde | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 17 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 26 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 39.7 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 49.5 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, SDS-PAGE | Kluyveromyces marxianus |
| 49000 | - |
4 * 49000, SDS-PAGE | Kluyveromyces marxianus |
| 190000 | - |
gel filtration | Kluyveromyces marxianus |
| 200000 | - |
gel filtration | Kluyveromyces marxianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | G3FFC9 | - |
- |
| Kluyveromyces marxianus | V9SCJ1 | - |
- |
| Kluyveromyces marxianus | V9SDP6 | - |
- |
| Kluyveromyces marxianus | V9SFA1 | - |
- |
| Kluyveromyces marxianus GX-UN120 | G3FFC9 | - |
- |
| Kluyveromyces marxianus GX-UN120 | V9SCJ1 | - |
- |
| Kluyveromyces marxianus GX-UN120 | V9SDP6 | - |
- |
| Kluyveromyces marxianus GX-UN120 | V9SFA1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADH + H+ | - |
Kluyveromyces marxianus | ethanol + NAD+ | - |
r | |
| acetaldehyde + NADH + H+ | - |
Kluyveromyces marxianus GX-UN120 | ethanol + NAD+ | - |
r | |
| ethanol + NAD+ | - |
Kluyveromyces marxianus | acetaldehyde + NADH + H+ | - |
r | |
| ethanol + NAD+ | - |
Kluyveromyces marxianus GX-UN120 | acetaldehyde + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48000, SDS-PAGE | Kluyveromyces marxianus |
| tetramer | 4 * 49000, SDS-PAGE | Kluyveromyces marxianus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH1 | - |
Kluyveromyces marxianus |
| ADH2 | - |
Kluyveromyces marxianus |
| ADH3 | - |
Kluyveromyces marxianus |
| ADH4 | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Kluyveromyces marxianus |
| 50 | - |
- |
Kluyveromyces marxianus |
| 55 | - |
- |
Kluyveromyces marxianus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 33 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 53 | - |
acetaldehyde | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 142 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 158 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 283 | - |
acetaldehyde | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Kluyveromyces marxianus |
| 8 | - |
- |
Kluyveromyces marxianus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Kluyveromyces marxianus | |
| NADH | - |
Kluyveromyces marxianus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Kluyveromyces marxianus | gene expression is largely induced during ethanol fermentation | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Adh1 is the primary alcohol dehydrogenase responsible for the production of ethanol from the reduction of acetaldehyde in Kluyveromyces marxianus | Kluyveromyces marxianus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 1.3 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 3.5 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 9.3 | - |
ethanol | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 71.7 | - |
acetaldehyde | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
| 450 | - |
acetaldehyde | pH 7.0, temperature not specified in the publication | Kluyveromyces marxianus | |
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