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Literature summary for 1.1.1.1 extracted from

  • Xiao, S.; Xu, J.; Chen, X.; Li, X.; Zhang, Y.; Yuan, Z.
    3-Methyl-1-butanol biosynthesis in an engineered Corynebacterium glutamicum (2016), Mol. Biotechnol., 58, 311-318.
    View publication on PubMed

Application

Application Comment Organism
synthesis alpha-ketoisovalerate decarboxylase Kivd from Lactococcus lactis combined with alcohol dehydrogenase Adh3 from Zymomonas mobilis are the optimum candidates for 3-methyl-1-butanol production in Corynebacterium glutamicum. The recombinant strain produces 0.182 g/l of 3-methyl-1-butanol and 0.144 g/l of isobutanol after 12 h of incubation. Further inactivation of the E1 subunit of pyruvate dehydrogenase complex gene (aceE) and lactic dehydrogenase gene (ldh) improves the 3-methyl-1-butanol titer to 0.497 g/l after 12 h of incubation Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
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-
-

Synonyms

Synonyms Comment Organism
ADH3
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Zymomonas mobilis