Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 15% inhibition at 1 mM | [Candida] maris | |
HgCl2 | complete inhibition at 1 mM | [Candida] maris | |
iodoacetate | 20% inhibition at 1 mM | [Candida] maris | |
MgSO4 | 19% inhibition at 1 mM | [Candida] maris | |
MnCl2 | 24% inhibition at 1 mM | [Candida] maris | |
additional information | no inhibition by 4-chloromercuribenzoate at 0.1 mM and by CuSO4 at 1 mM, poor effects by EDTA, 1,20-phenanthroline, 2-mercaptoethanol, and DTT | [Candida] maris | |
NEM | 17% inhibition at 1 mM | [Candida] maris | |
quercetin | 22% inhibition at 0.01 mM | [Candida] maris | |
ZnSO4 | 26% inhibition at 1 mM | [Candida] maris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28900 | - |
2 * 28900, SDS-PAGE | [Candida] maris |
59900 | - |
gel filtration | [Candida] maris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] maris | - |
- |
- |
[Candida] maris IFO10003 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native (R)-specific alcohol dehydrogenase 37fold from strain IFO10003, by ammonium sulfate fractionation, and anion exchange and hydrophobic interaction chromatography to homogeneity | [Candida] maris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.6 | - |
purified native enzyme, pH 6.5, 30°C | [Candida] maris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanal + NADH + H+ | - |
[Candida] maris | 1-butanol + NAD+ | - |
r | |
1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+ | - |
[Candida] maris | 1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+ | - |
r | |
1-hexanal + NADH + H+ | - |
[Candida] maris | 1-hexanol + NAD+ | - |
r | |
2,3'-dichloroacetophenone + NADH + H+ | - |
[Candida] maris | 2-chloro-1-(3-chlorophenyl)ethanol + NAD+ | - |
r | |
2,3'-dichloroacetophenone + NADH + H+ | - |
[Candida] maris IFO10003 | 2-chloro-1-(3-chlorophenyl)ethanol + NAD+ | - |
r | |
2-acetylcyclohexanone + NADH + H+ | - |
[Candida] maris | 2-acetylcyclohexanol + NAD+ | - |
r | |
2-acetylcyclopentanone + NADH + H+ | - |
[Candida] maris | 2-acetylcyclopentanol + NAD+ | - |
r | |
2-acetylfuran + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
2-acetylpyridine + NADH + H+ | - |
[Candida] maris | (R)-1-(2-pyridyl)ethanol + NAD+ | - |
r | |
2-acetylpyrrole + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
2-acetylthiazole + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
2-acetylthiophene + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
2-butanone + NADH + H+ | - |
[Candida] maris | 2-butanol + NAD+ | - |
r | |
2-chloroacetophenone + NADH + H+ | low activity | [Candida] maris | 1-(2-chlorophenyl)ethanol + NAD+ | - |
r | |
2-hexanone + NADH + H+ | - |
[Candida] maris | 2-hexanol + NAD+ | - |
r | |
2-nitrobenzaldehyde + NADH + H+ | low activity | [Candida] maris | 2-nitrobenzyl alcohol + NAD+ | - |
r | |
2-octanone + NADH + H+ | - |
[Candida] maris | 2-octanol + NAD+ | - |
r | |
2-oxopentanoate + NADH + H+ | low activity | [Candida] maris | 2-hydroxypentanoate + NAD+ | - |
r | |
2-pentanone + NADH + H+ | - |
[Candida] maris | 2-pentanol + NAD+ | - |
r | |
3-acetylpyridine + NADH + H+ | - |
[Candida] maris | (R)-1-(3-pyridyl)ethanol + NAD+ | - |
r | |
3-chlorobenzaldehyde + NADH + H+ | - |
[Candida] maris | 3-chlorobenzyl alcohol + NAD+ | - |
r | |
3-methylbutan-2-one + NADH + H+ | - |
[Candida] maris | 3-methyl-2-butanol + NAD+ | - |
r | |
3-nitroacetophenone + NADH + H+ | - |
[Candida] maris | 1-(3-nitrophenyl)ethanol + NAD+ | - |
r | |
3-nitrobenzaldehyde + NADH + H+ | - |
[Candida] maris | 3-nitrobenzyl alcohol + NAD+ | - |
r | |
4-acetylpyridine + NADH + H+ | - |
[Candida] maris | (R)-1-(4-pyridyl)ethanol + NAD+ | - |
r | |
4-chloroacetophenone + NADH + H+ | - |
[Candida] maris | 1-(4-chlorophenyl)ethanol + NAD+ | - |
r | |
4-chlorobenzaldehyde + NADH + H+ | - |
[Candida] maris | 4-chlorobenzyl alcohol + NAD+ | - |
r | |
4-fluoroacetophenone + NADH + H+ | - |
[Candida] maris | 1-(4-fluorophenyl)ethanol + NAD+ | - |
r | |
4-methylpentan-2-one + NADH + H+ | - |
[Candida] maris | 4-methyl-2-pentanol + NAD+ | - |
r | |
4-nitrobenzaldehyde + NADH + H+ | - |
[Candida] maris | 4-nitrobenzyl alcohol + NAD+ | - |
r | |
5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+ | - |
[Candida] maris | 5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+ | - |
r | |
5-acetylfuro[2,3-c]pyridine + NADH + H+ | - |
[Candida] maris | 5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
[Candida] maris | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
[Candida] maris IFO10003 | ethanol + NAD+ | - |
r | |
acetoin + NADH + H+ | - |
[Candida] maris | 3-hydroxy-2-butanol + NAD+ | - |
r | |
acetone + NADH + H+ | - |
[Candida] maris | 2-propanol + NAD+ | - |
? | |
acetophenone + NADH + H+ | - |
[Candida] maris | 1-phenylethanol + NAD+ | - |
r | |
acetophenone + NADH + H+ | - |
[Candida] maris IFO10003 | 1-phenylethanol + NAD+ | - |
r | |
acetylacetone + 2 NADH + 2 H+ | - |
[Candida] maris | 2,4-pentanediol + 2 NAD+ | - |
r | |
acetylpyrazine + NADH + H+ | - |
[Candida] maris | (R)-1-(pyrazyl)ethanol + NAD+ | - |
r | |
benzaldehyde + NADH + H+ | - |
[Candida] maris | benzyl alcohol + NAD+ | - |
r | |
benzaldehyde + NADH + H+ | - |
[Candida] maris IFO10003 | benzyl alcohol + NAD+ | - |
r | |
benzylacetone + NADH + H+ | - |
[Candida] maris | 4-phenylbutan-2-ol + NAD+ | - |
r | |
chloroacetone + NADH + H+ | - |
[Candida] maris | 1-chloro-2-propanol + NAD+ | - |
r | |
diacetyl + 2 NADH + 2 H+ | - |
[Candida] maris | 2,3-butandiol + 2 NAD+ | - |
r | |
diethylketone + NADH + H+ | - |
[Candida] maris | 3-pentanol + NAD+ | - |
r | |
ethyl 4-chloroacetoacetate + NADH + H+ | - |
[Candida] maris | ethyl 4-chloro-3-hydroxybutanoate + NAD+ | - |
r | |
ethyl acetoacetate + NADH + H+ | - |
[Candida] maris | ethyl 3-hydroxybutanoate + NAD+ | - |
r | |
ethyl pyruvate + NADH + H+ | - |
[Candida] maris | ethyl lactate + NAD+ | - |
r | |
methyl acetoacetate + NADH + H+ | - |
[Candida] maris | methyl 3-hydroxybutanoate + NAD+ | - |
r | |
methyl pyruvate + NADH + H+ | - |
[Candida] maris | methyl lactate + NAD+ | - |
r | |
additional information | substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol | [Candida] maris | ? | - |
? | |
additional information | substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol | [Candida] maris IFO10003 | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
propionaldehyde + NADH + H+ | - |
[Candida] maris | 1-propanol + NAD+ | - |
r | |
pyridine 2-aldehyde + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
pyridine 3-aldehyde + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r | |
pyridine 4-aldehyde + NADH + H+ | - |
[Candida] maris | ? + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28900, SDS-PAGE | [Candida] maris |
Synonyms | Comment | Organism |
---|---|---|
(R)-specific alcohol dehydrogenase | - |
[Candida] maris |
AFPDH | - |
[Candida] maris |
NADH-dependent alcohol dehydrogenase | - |
[Candida] maris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | [Candida] maris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 75 | activity range, profile overview | [Candida] maris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
purified native enzyme, stable up to | [Candida] maris |
40 | - |
purified enzyme, inactivation | [Candida] maris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | reduction reaction | [Candida] maris |
8.5 | - |
oxidation reaction | [Candida] maris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | oxidation reaction activity range, profile overview | [Candida] maris |
5.5 | 9 | reduction reaction activity range, profile overview | [Candida] maris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] maris | |
NADH | required | [Candida] maris |