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Literature summary for 1.1.1.1 extracted from

  • Guo, X.; Feng, Y.; Wang, X.; Liu, Y.; Liu, W.; Li, Q.; Wang, J.; Xue, S.; Zhao, Z.K.
    Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase (2019), Bioorg. Med. Chem. Lett., 29, 1446-1449 .
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.26
-
 iso-propanol pH 7.5, 23°C Geobacillus stearothermophilus
2.45
-
 1-butanol pH 7.5, 23°C Geobacillus stearothermophilus
2.95
-
 ethanol pH 7.5, 23°C Geobacillus stearothermophilus
23.64
-
 1,3-Propanediol pH 7.5, 23°C Geobacillus stearothermophilus
23.82
-
 2-butanol pH 7.5, 23°C Geobacillus stearothermophilus
39.8
-
 1-propanol pH 7.5, 23°C Geobacillus stearothermophilus
294.23
-
 methanol pH 7.5, 23°C Geobacillus stearothermophilus
568.84
-
 tert-butanol pH 7.5, 23°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P42328
-
-
Geobacillus stearothermophilus DSM 2334 P42328
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + NAD+
-
 Geobacillus stearothermophilus ? + NADH + H+
-
 ?
1,3-propanediol + NAD+
-
 Geobacillus stearothermophilus DSM 2334 ? + NADH + H+
-
 ?
1-butanol + NAD+
-
 Geobacillus stearothermophilus butyraldehyde + NADH + H+
-
 ?
1-butanol + NAD+
-
 Geobacillus stearothermophilus DSM 2334 butyraldehyde + NADH + H+
-
 ?
1-propanol + NAD+
-
 Geobacillus stearothermophilus propionaldehyde + NADH + H+
-
 ?
2-butanol + NAD+
-
 Geobacillus stearothermophilus 2-butanone + NADH + H+
-
 ?
2-butanol + NAD+
-
 Geobacillus stearothermophilus DSM 2334 2-butanone + NADH + H+
-
 ?
ethanol + NAD+
-
 Geobacillus stearothermophilus acetaldehyde + NADH + H+
-
 ?
ethanol + NAD+
-
 Geobacillus stearothermophilus DSM 2334 acetaldehyde + NADH + H+
-
 ?
iso-propanol + NAD+
-
 Geobacillus stearothermophilus ? + NADH + H+
-
 ?
methanol + NAD+
-
 Geobacillus stearothermophilus formaldehyde + NADH + H+
-
 ?
methanol + NAD+
-
 Geobacillus stearothermophilus DSM 2334 formaldehyde + NADH + H+
-
 ?
tert-butanol + NAD+
-
 Geobacillus stearothermophilus butyraldehyde + NADH + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 1-butanol pH 7.5, 23°C Geobacillus stearothermophilus
0.013
-
 methanol pH 7.5, 23°C Geobacillus stearothermophilus
0.019
-
 ethanol pH 7.5, 23°C Geobacillus stearothermophilus
0.019
-
 1-propanol pH 7.5, 23°C Geobacillus stearothermophilus
0.026
-
 tert-butanol pH 7.5, 23°C Geobacillus stearothermophilus
0.041
-
 1,3-Propanediol pH 7.5, 23°C Geobacillus stearothermophilus
0.076
-
 iso-propanol pH 7.5, 23°C Geobacillus stearothermophilus
0.094
-
 2-butanol pH 7.5, 23°C Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000044
-
 methanol pH 7.5, 23°C Geobacillus stearothermophilus
0.000046
-
 tert-butanol pH 7.5, 23°C Geobacillus stearothermophilus
0.00046
-
 1-propanol pH 7.5, 23°C Geobacillus stearothermophilus
0.00167
-
 1-butanol pH 7.5, 23°C Geobacillus stearothermophilus
0.00171
-
 1,3-Propanediol pH 7.5, 23°C Geobacillus stearothermophilus
0.00396
-
 2-butanol pH 7.5, 23°C Geobacillus stearothermophilus
0.00626
-
 ethanol pH 7.5, 23°C Geobacillus stearothermophilus
0.06027
-
 iso-propanol pH 7.5, 23°C Geobacillus stearothermophilus