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Literature summary for 1.1.1.1 extracted from
- Purification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans (1985), Biochem. J., 225, 449-454.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
acetaldehyde | - |
Aspergillus nidulans |  |
| 0.35 | - |
NADH | - |
Aspergillus nidulans | |
| 0.9 | - |
NAD+ | reduction of ethanol | Aspergillus nidulans | |
| 1.3 | - |
ethanol | - |
Aspergillus nidulans | |
| 1.3 | - |
propan-2-ol | - |
Aspergillus nidulans | |
| 550 | - |
NAD+ | reduction of propan-2-ol | Aspergillus nidulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | contains 1 zinc atom per subunit | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37500 | - |
x * 37500, SDS-PAGE | Aspergillus nidulans |
| 290000 | - |
gel filtration | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADH + H+ | - |
Aspergillus nidulans | ethanol + NAD+ | - |
r | |
| ethanol + NAD+ | - |
Aspergillus nidulans | acetaldehyde + NADH | - |
r | |
| propan-2-ol + NAD+ | - |
Aspergillus nidulans | acetone + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37500, SDS-PAGE | Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
reduction of propan-2-ol or ethanol | Aspergillus nidulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Aspergillus nidulans | |
| NADH | - |
Aspergillus nidulans | |
| NADP+ | no activity as coenzyme | Aspergillus nidulans | |
| NADPH | no activity as coenzyme | Aspergillus nidulans | |
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Release 2023.1 (January 2023)
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