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Literature summary for 1.1.1.1 extracted from

  • Rosell, A.; Valencia, E.; Ochoa, W.F.; Fita, I.; Pares, X.; Farres, J.
    Complete reversal of coenzyme specificity by concerted mutation of three consecutive residues in alcohol dehydrogenase (2003), J. Biol. Chem., 278, 40573-40580.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme-NADP+-cofactor complex, X-ray diffraction strcuture determination and analysis, computational structure modeling Pelophylax perezi

Protein Variants

Protein Variants Comment Organism
G223D unaltered cofactor specificity compared to the wild-type enzyme Pelophylax perezi
G223D/T224I highly reduced activity with NADP+ compared to the wild-type enzyme, wild-type-like activity with NAD+ Pelophylax perezi
G223D/T224I/H225N altered cofactor specificity, highly reduced activity with NADP+ compared to the wild-type enzyme, wild-type-like activity with NAD+ Pelophylax perezi
H225N unaltered cofactor specificity compared to the wild-type enzyme Pelophylax perezi
T224I unaltered cofactor specificity compared to the wild-type enzyme Pelophylax perezi

Organism

Organism UniProt Comment Textmining
Pelophylax perezi
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
Pelophylax perezi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
wild-type and mutant enzymes Pelophylax perezi

Synonyms

Synonyms Comment Organism
ADH8
-
Pelophylax perezi

Cofactor

Cofactor Comment Organism Structure
NAD+ activity with mutants G223D/T224I and G223D/T224I/H225N Pelophylax perezi
NADH activity with mutants G223D/T224I and G223D/T224I/H225N Pelophylax perezi
NADP+ specific for, residues Gly223, Thr224, His225, Leu200, and Lys228 are involved in cofactor binding pocket formation Pelophylax perezi
NADPH specific for, residues Gly223, Thr224, His225, Leu200, and Lys228 are involved in cofactor binding pocket formation Pelophylax perezi