Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | strong product inhibition | Thermomicrobium roseum | |
imidazole | weak inhibition | Thermomicrobium roseum | |
pyrazole | strong inhibition | Thermomicrobium roseum | |
Pyridine | - |
Thermomicrobium roseum | |
trichloroethanol | weak inhibition | Thermomicrobium roseum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.2 | - |
ethanol | pH 10.0, 50°C | Thermomicrobium roseum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Thermomicrobium roseum |
88000 | - |
approximately, gel filtration | Thermomicrobium roseum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomicrobium roseum | - |
ATCC 27502 | - |
Purification (Comment) | Organism |
---|---|
128fold to homogeneity by adenosine 5'-monophosphate affinity and Mono-Q ion exchange chromatography | Thermomicrobium roseum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.28 | - |
purified enzyme | Thermomicrobium roseum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2,2-trichloroethanol + NAD+ | - |
Thermomicrobium roseum | trichloroacetaldehyde + NADH + H+ | - |
? | |
crotyl alcohol + NAD+ | - |
Thermomicrobium roseum | crotonaldehyde + NADH | - |
? | |
ethanol + NAD+ | best substrate | Thermomicrobium roseum | acetaldehyde + NADH | - |
r | |
methanol + NAD+ | low activity | Thermomicrobium roseum | formaldehyde + NADH + H+ | - |
? | |
additional information | enzyme shows high substrate specificity towards primary aliphatic alcohols, no activity with 2-butanol, tert-butanol, isoamyl alcohol, isobutyl alcohol, 1,6-hexadiol, and mono-, di-, and triethanolamine | Thermomicrobium roseum | ? | - |
? | |
n-butanol + NAD+ | low activity | Thermomicrobium roseum | butanal + NADH | - |
? | |
n-propanol + NAD+ | - |
Thermomicrobium roseum | propionaldehyde + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Thermomicrobium roseum |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Thermomicrobium roseum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermomicrobium roseum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 85 | maximal activity at 70°C, 60% of maximal activity at 85°C | Thermomicrobium roseum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable upt to | Thermomicrobium roseum |
75 | - |
and above, rapid loss of activity | Thermomicrobium roseum |
85 | - |
slight residual activity | Thermomicrobium roseum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Thermomicrobium roseum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermomicrobium roseum | |
NADH | - |
Thermomicrobium roseum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomicrobium roseum | - |
- |
6 |