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Literature summary for 1.1.1.1 extracted from

  • Wang, N.; Shi, H.; Yao, Q.; Zhou, Y.; Kang, L.; Chen, H.; Chen, K.
    Cloning, expression and characterization of alcohol dehydrogenases in the silkworm Bombyx mori (2011), Genet. Mol. Biol., 34, 240-243.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged BmADH protein in Escherichia coli strain BL21 (DE3) Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, recombinant His6-tagged enzyme, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NAD+ Bombyx mori
-
 acetaldehyde + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Bombyx mori Q1G151
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble recombinant His6-tagged BmADH protein from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Bombyx mori
-
epidermis
-
 Bombyx mori
-
fat body
-
 Bombyx mori
-
ganglion
-
 Bombyx mori
-
hemocyte
-
 Bombyx mori
-
hindgut
-
 Bombyx mori
-
malpighian tubule
-
 Bombyx mori
-
midgut
-
 Bombyx mori
-
additional information tissue expression profile of fifth instar larvae, overview Bombyx mori
-
ovary
-
 Bombyx mori
-
silk gland
-
 Bombyx mori
-
sperm
-
 Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
 Bombyx mori acetaldehyde + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 31000, recombinant His6-tagged enzyme, SDS-PAGE Bombyx mori

Synonyms

Synonyms Comment Organism
ADH
-
 Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 purified recombinant His-tagged enzyme, 10 min, completely stable up to Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 9 purified recombinant His-tagged enzyme, 10 min, high stability within this range Bombyx mori

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bombyx mori
NADH
-
 Bombyx mori