Information on EC 3.1.3.16 - protein-serine/threonine phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
3.1.3.16
-
RECOMMENDED NAME
GeneOntology No.
protein-serine/threonine phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
mechanism of calcineurin catalysed reaction
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
catalytic mechanism, PstPpp catalytic domain structure and active site structure, structure-function relationship
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
Glu76 is involved in catalysis, active site structure
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
mechanism of isozyme PP1, the enzyme also performs the reaction of EC 3.1.3.53, myosin-light-chain-phosphatase
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
mechanism of PP1
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
structural basis for the catalytic activity, the catalytic motif Asp271-metal1:metal2-water1-His427-His304-Asp274 requires precise substrate positioning, catalytic mechanism, metal-mediated substrate hydrolysis
P36873
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
the enzyme also performs the reaction of EC 3.1.3.53, myosin-light-chain-phosphatase
-
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
the enzymes of Synechocystis sp. also perform the protein tyrosine phosphatase reaction, EC 3.1.3.48
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
protein-serine/threonine-phosphate phosphohydrolase
A group of enzymes removing the serine- or threonine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes that have been phosphorylated under the action of a kinase (cf. EC 3.1.3.48 protein-tyrosine-phosphatase). The spleen enzyme also acts on phenolic phosphates and phosphamides (cf. EC 3.9.1.1, phosphoamidase).
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3-hydroxy 3-methylglutaryl CoenzymeA reductase phosphatase
-
-
-
-
Aspergillus awamori acid protein phosphatase
-
-
-
-
BCKDH phosphatase
-
-
-
-
branched-chain alpha-keto acid dehydrogenase phosphatase
-
-
-
-
calcineurin
-
-
-
-
Calcineurin A1
-
-
-
-
Calcineurin A2
-
-
-
-
CaM-kinase phosphatase
-
-
-
-
CaMKPase
-
-
-
-
casein phosphatase
-
-
-
-
DRES10
-
-
-
-
Fibroblast growth factor inducible protein 13
-
-
-
-
FIN13
-
-
-
-
Flap wing protein
-
-
-
-
HMG-CoA reductase phosphatase
-
-
-
-
Magnesium-dependent calcium inhibitable phosphatase
-
-
-
-
MCPP
-
-
-
-
Microtubule star protein
-
-
-
-
phosphatase 2A
-
-
-
-
phosphatase 2B
-
-
-
-
phosphatase C-II
-
-
-
-
Phosphatase esp1
-
-
-
-
phosphatase H-II
-
-
-
-
phosphatase I
-
-
-
-
phosphatase IB
-
-
-
-
phosphatase II
-
-
-
-
phosphatase III
-
-
-
-
phosphatase IV
-
-
-
-
phosphatase SP
-
-
-
-
phosphoprotein phosphatase
-
-
-
-
phosphopyruvate dehydrogenase phosphatase
-
-
-
-
phosphospectrin phosphatase
-
-
-
-
PK-Pase
-
-
-
-
polycation modulated (PCM-) phosphatase
-
-
-
-
PP-1A
-
-
-
-
PP-1B
-
-
-
-
PP-1G
-
-
-
-
PP2A-alpha
-
-
-
-
PP2A-beta
-
-
-
-
PP2C
-
-
-
-
PP2C-alpha
-
-
-
-
PP2C-beta
-
-
-
-
PP2C-delta
-
-
-
-
PP2C-gamma
-
-
-
-
Pp4
-
-
-
-
PP5
-
-
-
-
PP6
-
-
-
-
PPEF
-
-
-
-
PPN
-
-
-
-
PPT
-
-
-
-
protein D phosphatase
-
-
-
-
protein phosphatase
-
-
-
-
Protein phosphatase 1A
-
-
-
-
Protein phosphatase 1B
-
-
-
-
Protein phosphatase 1C
-
-
-
-
Protein phosphatase magnesium-dependent 1 delta
-
-
-
-
Protein phosphatase magnesium-dependent 1 gamma
-
-
-
-
Protein phosphatase with EF calcium-binding domain
-
-
-
-
PSPase
-
-
-
-
Retinal degeneration C protein
-
-
-
-
Suppressor protein SDS21
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9025-75-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
different mutants: mutations in genes involved in the ethylene signal transduction pathway and a mutation at the start of exon 2
UniProt
Manually annotated by BRENDA team
ecotype Col-0
-
-
Manually annotated by BRENDA team
ecotype Columbia
-
-
Manually annotated by BRENDA team
catalytic subunit
Uniprot
Manually annotated by BRENDA team
tissue-specific isozymes in eye and brain
Uniprot
Manually annotated by BRENDA team
mollusc, phosphatase type 2C
-
-
Manually annotated by BRENDA team
subunits A and B are encoded by genes tax-6 and cnb-1
-
-
Manually annotated by BRENDA team
strain SC5314
-
-
Manually annotated by BRENDA team
strain SC5314
UniProt
Manually annotated by BRENDA team
chicken anemia virus VP2
-
-
-
Manually annotated by BRENDA team
Enterococcus faecium D344S
-
-
-
Manually annotated by BRENDA team
strain 7600
Uniprot
Manually annotated by BRENDA team
isozyme delta; PP1 isozyme delta
SwissProt
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
marine dinoflagellate
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
; isozyme PP2Cepsilon
SwissProt
Manually annotated by BRENDA team
catalytic subunit
Uniprot
Manually annotated by BRENDA team
individuals with Down syndrome
-
-
Manually annotated by BRENDA team
isoform PPP1CA
Uniprot
Manually annotated by BRENDA team
isoform PPP1CB
UniProt
Manually annotated by BRENDA team
isoform PPP1CC
Uniprot
Manually annotated by BRENDA team
isozyme PP2Cdelta
-
-
Manually annotated by BRENDA team
isozymes PP1alpha, PP1beta, and PP1gamma1
-
-
Manually annotated by BRENDA team
mitochondrial precursor
UniProt
Manually annotated by BRENDA team
protein phosphatase 3 regulatory subunit B beta isoform; gene PPP3RL, two isozymes PPP3R1 and PPP3R2
SwissProt
Manually annotated by BRENDA team
several isozymes, classification, overview
-
-
Manually annotated by BRENDA team
C57Bl6 mice
-
-
Manually annotated by BRENDA team
Down syndrome model Ts65Dn mice
-
-
Manually annotated by BRENDA team
male BALB/c mice
-
-
Manually annotated by BRENDA team
several isozymes, classification, overview
-
-
Manually annotated by BRENDA team
Mus musculus C57BL6
C57Bl6 mice
-
-
Manually annotated by BRENDA team
regulatory subunit delta, isozyme PP2Adelta; isozyme PP2Adelta
SwissProt
Manually annotated by BRENDA team
isozyme PP2A-5; ssp. indica, var. IR36, 5 isozymes of PP2A, PP2A1-5, each containing a different subunit
SwissProt
Manually annotated by BRENDA team
isozyme PP2A2; ssp. indica, var. IR36, 5 isozymes of PP2A, PP2A1-4, each containing a different subunit
SwissProt
Manually annotated by BRENDA team
isozyme PP2A4; ssp. indica, var. IR36, 5 isozymes of PP2A, PP2A1-4, each containing a different subunit
SwissProt
Manually annotated by BRENDA team
strain PAO1, gene PA3346
-
-
Manually annotated by BRENDA team
Pyrodictium abyssi TAG11
-
UniProt
Manually annotated by BRENDA team
female and male Wistar rats; regucalcin-expressing transgenic Sprague-Dawley rats and wild-type male Wistar rats
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
Uniprot
Manually annotated by BRENDA team
Sprague-Dawley rat
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
several strains
-
-
Manually annotated by BRENDA team
strain AY925
-
-
Manually annotated by BRENDA team
strains BY4741 and W303
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae AY925
strain AY925
-
-
Manually annotated by BRENDA team
serovar Typhi, strains CT18 and Ty2
-
-
Manually annotated by BRENDA team
Schizosaccharomyces pombe MI200
strain MI200
-
-
Manually annotated by BRENDA team
cultivar VFNT Cherry
-
-
Manually annotated by BRENDA team
the wild type strain DSM 639 missing 322 bp called MW001
UniProt
Manually annotated by BRENDA team
Sulfolobus acidocaldarius MW001
the wild type strain DSM 639 missing 322 bp called MW001
UniProt
Manually annotated by BRENDA team
beta catalytic subunit
UniProt
Manually annotated by BRENDA team
strain PCC 6803, genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1
-
-
Manually annotated by BRENDA team
strain SM 427
-
-
Manually annotated by BRENDA team
Trypanosoma brucei SM 427
strain SM 427
-
-
Manually annotated by BRENDA team
cultivar Ile de France
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
calcineurin inhibition by FK506 significantly enhances the phosphorylation of protein tau at Ser262, Ser198, Ser199, and/or Ser202 and Ser396, and/or Ser404, overview
malfunction
-
calcineurin inhibition in fibroblasts and keratinocytes may be of influence on the overall functioning of the skin immune system
malfunction
-
calcineurin is involved in and a therapeutic target in myasthenia gravis, clinical parameters, overview
malfunction
-
calcineurin participates in neuronal apoptosis, protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin
malfunction
-
DSCR1-dependent inhibition of the VEGF-calcineurin-NFAT pathway in endothelial cells as a key component of the reduced cancer incidence in Down syndrome individuals
malfunction
-
malfunctions of C-kinase-activated PP1 inhibitor proteins are linked to a variety of diseases, including cardiovascular disease and cancer
malfunction
-
modest elevation in expression afforded by a single extra transgenic copy of Dscr1 is sufficient to confer significant suppression of tumor growth in mice and that such resistance is a consequence of a deficit in tumor angiogenesis arising from suppression of the calcineurin pathway. Attenuation of calcineurin activity by DSCR1 together with another chromosome 21 gene DYRK1A, may be sufficient to dramatically diminish angiogenesis
malfunction
-
partly malignancy-dependent role of calcineurin in melanoma cell lines, overview. Calcineurin-inhibiting compounds are applied for local treatment of psoriasis or atopic dermatitis
malfunction
-
perturbations in the regulation of PP1 by inhibitor-1 have been implicated in the pathogenesis of heart failure, role of PP1 and protein phosphatase-1 inhibitor-1 in the failing heart, overview
malfunction
-
a decrease in alpha-isoform of PP2A catalytic subunit accelerates osteoblast differentiation through the expression of bone-related genes
malfunction
-
inhibition of protein phosphatase 2A results in changes in the organization of endothelial cell cytoskeleton as microtubule dissolution and actin re-arrangement are detected. Depletion of Balpha regulatory subunit of protein phosphatase 2A has similar effect on the cytoskeleton structure of the cells
malfunction
-
knockdown of protein phosphatase PP1-3 leads to a coordinated rearrangement of cellular organelles and compartments in the procyclic trypanosome
metabolism
-
the enzyme is involved in the Raf-1/ERK pathway, overview
metabolism
-
Ser/Thr phosphatase Ppq1 down-regulates mating signaling by targeting at or upstream of the terminal MAP kinase Fus3 in the cascade
physiological function
-
PGAM5 is an evolutionarily conserved activator of apoptosis signal-regulating kinase 1
physiological function
-
Ca2+-calcineurin signaling
physiological function
-
calcineurin is one of the target molecules regulated by the changes of intracellular Ca2+ level, it has a regulatory role in Ca2+ cytosolic concentration and signaling in chondrogenic cells, Ca2+ plays an important role in chondrogenesis, cartilage formation and cartilage differentiation, overview
physiological function
-
calcineurin play roles in various signaling pathways such as fertility, movement, body size regulation and serotonin-mediated egg laying
physiological function
-
calcineurin plays an important role in brain function and in regulation of tau phosphorylation
physiological function
-
opposing the protein kinase A axis are protein phosphatases that dephosphorylate regulatory proteins, e.g. ryanodine receptor and phospholamban in sarcoplasmic reticulum, troponin I and C-protein in the myofilaments, and the plasma membrane L-type calcium channel, and play an essential role in maintaining biochemical and functional phosphorylation homeostasis, overview
physiological function
-
PP1 phosphorylation is essential for proper cell cycle progression. NIPP1-associated PP1 regulates Sap155 phosphorylation, overview
physiological function
-
the Ca2+/calmodulin-dependent protein phosphatase calcineurin is a key mediator in antigen-specific T-cell activation
physiological function
-
the enzyme is involved in regulation of diverse enzyme functions via de-/activation of phosphoproteins through dephosphorylation, in the same way its inhibitor protein phosphatase-1 inhibitor-1, phosphoproteomic analysis, overview
physiological function
-
topical calcineurin inhibits DNA repair and reduces apoptosis in epidermal keratinocytes
physiological function
-
type 1 protein phosphatase is a critical negative regulator of Ca2+ cycling and contractility in the cardiomyocyte. In particular, it mediates restoration of cardiac function to basal levels, after beta-adrenergic stimulation, by dephosphorylating key phospho-proteins, role of PP1 in the heart and its critical regulation by the endogenous phospho-protein I-1, overview
physiological function
-
cytoplasmic accumulation of the enzyme induces Ebola virus transcription
physiological function
P36873, P62136, P62140
isofom PPP1CC1 participates in actin-related function, mitosis, brain function, vesicular trafficking, and other functions
physiological function
P36873
isoform PP1 plays critical roles in an enormous variety of cellular processes such as cell cycle progression, protein synthesis, carbohydrate metabolism and apoptosis
physiological function
P36873, P62136, P62140
isoform PPP1CA participates in nuclear function, androgen receptor signaling, TGFbeta signaling, protein synthesis, cell cylcle/apoptosis, brain function, actin-related function, centrosomal and other functions
physiological function
P36873, P62136, P62140
isoform PPP1CB participates in muscle function, glycogen metabolism, nuclear function, cell shortening and spreading, and cellular homeostasis
physiological function
-
overexpression of subunit PP2A Calpha reduces alkaline phosphatase activity. Osteoblast differentiation and mineralization are decreased in subunit PP2A Calpha-overexpressing cells, with reduction of bone-related genes including osterix, bone sialoprotein, and osteocalcin
physiological function
-
PP1 regulates diverse, essential cellular processes such as cell cycle progression, protein synthesis, muscle contraction, carbohydrate metabolism, transcription and neuronal signaling
physiological function
-
PP5 enhances thermotolerance via forming multi-chaperone complexes with heat shock protein 90.2 under heat shock conditions (42C) in Arabidopsis
physiological function
-
protein phosphatase 1 participates in cell cycle regulation, salinity tolerance, cytokinin production, embryo development, blue-light signaling, cell differentiation, cell patterning, cell division, and gene silencing. Protein phosphatase 2A is intimately involved in regulating intracellular responses to brassinosteroids. Functions of PP5 include roles in disease resistance, thermotolerance, and light detection, while PP7 is implicated in several sensory functions, in particular, light sensing through the regulation of cryptochrome and phytochrome
physiological function
-
protein phosphatase PP1-3 is essential in conserving the intracellular organisation of the procyclic trypanosome cell
physiological function
-
the enzyme is essential for asexual development and plant infection
physiological function
-
the enzyme plays a significant role in the maintenance of endothelial cell cytoskeleton and barrier function with special focus on the Balpha (regulatory) subunit of protein phosphatase 2A. The PP2A Balpha regulatory subunit associates with adherent junction proteins
physiological function
-
the enzyme is essential for asexual development and plant infection
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
14-3-3epsilon phosphoprotein + H2O
14-3-3epsilon protein + phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + methylphosphonate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
P62136
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
P48452
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Q4QE27
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
P67775
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Enterococcus faecium D344S
-
-
-
-
?
4-nitrophenyl phosphate + H2O
nitrophenol + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
4-nitrophenyl phosphate + H2O
nitrophenol + phosphate
show the reaction diagram
Sulfolobus acidocaldarius MW001
Q4JAD1, Q4JB88
-
-
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
6,8-difluoro-4-methylumbelliferone + phosphate
show the reaction diagram
-
-
-
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
6,8-difluoro-4-methylumbelliferone + phosphate
show the reaction diagram
-
-
-
-
?
a phosphoprotein + H2O
dephosphorylated protein + phosphate
show the reaction diagram
-
-
-
-
?
a phosphoprotein + H2O
a protein + phosphate
show the reaction diagram
-
the enzyme is specific from serinel/threonine-phosphorylated proteins including casein, histone H2a, phosphorylase kinase, or glycogen phosphorylase. The enzyme does not display detectable protein phosphatase activity toward a number of tyrosine-phosphorylated substrates
-
-
?
Ala-Arg-Thr-Cys-Arg-Ser-Gly-Ser(P)-Val-Tyr + H2O
Ala-Arg-Thr-Cys-Arg-Ser-Gly-Ser-Val-Tyr + phosphate
show the reaction diagram
-
best substrate
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
beta-naphthyl phosphate + H2O
beta-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
biotin-DGDFEEIPEE(P)YLQNH2 + H2O
biotin-DGDFEEIPEEYLQNH2 + phosphate
show the reaction diagram
-
-
-
-
?
carrier protein-p53-phosphoserine + H2O
carrier protein-p53-serine + phosphate
show the reaction diagram
-
-
-
-
?
carrier protein-PB1-phosphoserine + H2O
carrier protein-PB1-serine + phosphate
show the reaction diagram
-
-
-
-
?
carrier protein-T3-phosphothreonine + H2O
carrier protein-T3-threonine + phosphate
show the reaction diagram
-
-
-
-
?
carrier protein-T4-phosphothreonine + H2O
carrier protein-T4-threonine + phosphate
show the reaction diagram
-
-
-
-
?
carrier protein-T5-phosphothreonine + H2O
carrier protein-T5-threonine + phosphate
show the reaction diagram
-
-
-
-
?
chicken acidic leucine-rich epidermal growth factor-like domain-containing brain protein/neuroglycan C + H2O
?
show the reaction diagram
-
the regulatory subunit of protein phosphatase 2A, B56beta, interacts with chicken acidic leucine-rich epidermal growth factor-like domain-containing brain protein/neuroglycan C (CALEB/NGC) and inhibits CALEB/NGC-mediated dendritic branching but not spine formation
-
-
?
DADEpYLPQQG + H2O
DADEYLPQQG + phosphate
show the reaction diagram
chicken anemia virus, chicken anemia virus VP2
-
-
-
-
?
DLDVPIPGRFDRRVS(P)VAAE + H2O
DLDVPIPGRFDRRVSVAAE + phosphate
show the reaction diagram
-
-
-
-
?
DLDVPIPGRFDRRVSVAAE + H2O
? + phosphate
show the reaction diagram
-
peptide sequence corresponding to the R11 subunit of cAMP-dependent protein kinase
-
-
?
DLDVPIPGRFDRRVY(P)VAAE + H2O
DLDVPIPGRFDRRVYVAAE + phosphate
show the reaction diagram
-
-
-
-
?
DRRVS(P)VAAE + H2O
DRRVSVAAE + phosphate
show the reaction diagram
-
-
-
-
?
DRVY(P)IHPFHL + H2O
DRVYIHPFHL + phosphate
show the reaction diagram
-
-
-
-
?
ENDpYINASL + H2O
ENDYINASL + phosphate
show the reaction diagram
chicken anemia virus, chicken anemia virus VP2
-
-
-
-
?
ETTYERW(pT)TITQRER + H2O
ETTYERWTTITQRER + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
FDRRVS(P)VAAE + H2O
FDRRVSVAAE + phosphate
show the reaction diagram
-
-
-
-
?
FLRT(P)SCG + H2O
FLRTSCG + phosphate
show the reaction diagram
-
-
-
-
?
FLRTS(P)CG + H2O
FLRTSCG + phosphate
show the reaction diagram
-
-
-
-
?
FLRTT(P)CG + H2O
FLRTTCG + phosphate
show the reaction diagram
-
-
-
-
?
fluorotyrosine phosphate + H2O
fluorotyrosine + phosphate
show the reaction diagram
-
-
-
-
?
fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase + H2O
fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase + phosphate
show the reaction diagram
-
heterotrimeric phosphatase 2A catalyzing the dephosphorylation of fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
-
-
?
FYDEEVDEMY(P)REAPIDKKGNFNY(P)VEFTR + H2O
FYDEEVDEMYREAPIDKKGNFNYVEFTR + phosphate
show the reaction diagram
-
-
-
-
?
glycogen synthase + H2O
glycogen synthase + phosphate
show the reaction diagram
-
-
-
-
?
glycogen synthase + H2O
glycogen synthase + phosphate
show the reaction diagram
-
serine-phosphorylated glycogen synthase
-
-
?
glycogen synthase b + H2O
glycogen synthase I + phosphate
show the reaction diagram
-
-
-
-
?
glycogen synthase D + H2O
glycogen synthase I + phosphate
show the reaction diagram
-
-
-
-
?
glycogen synthase D + H2O
glycogen synthase I + phosphate
show the reaction diagram
-
-
-
-
r
histone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
KRpTIRR + H2O
KRTIRR + phosphate
show the reaction diagram
-
-
-
-
?
KRpTIRR + H2O
KRTIRR + phosphate
show the reaction diagram
-
-
-
-
?
myelin basic phosphoprotein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
myelin basic protein + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myosin light chain phosphate + H2O
myosin light chain + phosphate
show the reaction diagram
-
myosin light chain phosphatase is a specific form of PP1
-
-
?
NIDAIRA(pS)LNIMSR + H2O
NIDAIRASLNIMSR + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
O15920
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Q8N3J5
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
very low activity
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Q9U493
very low activity
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
very low activity
-
-
?
p-nitrophenyl phosphate + H2O
nitrophenol + phosphate
show the reaction diagram
P53042
-
-
-
?
p-nitrophenyl phosphate + H2O
nitrophenol + phosphate
show the reaction diagram
Q84K11
-
-
-
?
p38 MAP kinase phosphoprotein
?
show the reaction diagram
-
-
-
-
?
peptide mGluR3 + H2O
?
show the reaction diagram
P35813
-
-
-
?
Phe-Arg-Arg-Leu-Ser(P)-Ile-Ser + H2O
Phe-Arg-Arg-Leu-Ser-Ile-Ser + phosphate
show the reaction diagram
-
least effective substrate, corresponding to the alpha-subunit of protein phosphatase
-
-
?
phosphatidylinositol-(3,4,5)-triphosphate + H2O
?
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
the enzyme displays activity towards the D3 position of the phosphoinositide second messenger phosphatidylinositol (3,4,5)-triphosphate
-
-
?
phospho-Cdk2 + H2O
Cdk6 + phosphate
show the reaction diagram
-
-
-
-
?
phospho-Cdk6 + H2O
Cdk6 + phosphate
show the reaction diagram
-
-
-
-
?
phospho-D,L-tyrosine + H2O
D,L-tyrosine + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-serine + H2O
L-serine + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-threonine + H2O
L-threonine + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
show the reaction diagram
-
-
-
-
-
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
show the reaction diagram
-
preferred substrate
-
-
?
phospho-MKK + H2O
MKK + phosphate
show the reaction diagram
-
-
-
-
?
phospho-PA3347 + H2O
PA3347 + phosphate
show the reaction diagram
-
dephosphorylation of Ser56 by PA3346
-
-
?
phospho-TAK1 + H2O
TAK1 + phosphate
show the reaction diagram
-
-
-
-
?
phospho-threonylpeptide + H2O
threonylpeptide + phosphate
show the reaction diagram
-
-
-
-
?
phosphoangiotensin + H2O
angiotensin + phosphate
show the reaction diagram
-
phosphorylated at Tyr
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
Q55059
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphomyosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
-
-
-
?
phosphomyosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
-
-
-
?
phosphomyosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
-
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
O15920
specific for alpha-subunit
-
-
-
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphoprotamine + H2O
protamine + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotamine + H2O
protamine + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
Q5AME8
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P36873, P62136, P62140
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P48452
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P36873
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
Q4QE27
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P67775
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
U5PYV4
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
role of phosphatases in smooth muscle contractile systems
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
involved in the intracellular mechanism through which adrenocortical steroidogenesis is regulated, acting at a point after cAMP generation and action but proximal to the side-chain cleavage of cholesterol
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
modulation of pyruvate dehydrogenase complex
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
phosphatase PP3 stimulates the initiation of DNA-synthesis
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
involved in regulating exocytosis from the rat parotid gland via a cAMP-mediated process
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
control of cholesterol genesis
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
role in cAMP catabolism
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
capable of dephosphorylating the major proteins involved in control of glycogen metabolism, glycolysis, gluconeogenesis, aromatic amino acid breakdown, fatty acid, cholesterol, and protein synthesis
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein K15F + H2O
protein K15F + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein R20A + H2O
protein R20A + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein RII + H2O
protein RII + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
Q9XZE5
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
O15920
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
A8DQZ8
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
comparison of amino acid sequence of substrates at phosphorylated sites
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
hydrolyzes phosphoseryl, phosphotyrosyl and phosphothreonyl substrates
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
most of the substrates are phosphorylated by cAMP and cGMP dependent protein kinases, other substrates are phosphorylated by calmodulin dependent multiprotein kinase
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphopyruvate kinase + H2O
pyruvate kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphopyruvate kinase + H2O
pyruvate kinase + phosphate
show the reaction diagram
-
regulation of protein kinase during the transition to anoxia and back to the normoxic state
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
-
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
?
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase a + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylase a + H2O
phosphorylase a + phosphate
show the reaction diagram
-
-
-
-
r
phosphorylase a + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated 3-hydroxy 3-methylglutaryl coenzyme A reductase + H2O
3-hydroxy 3-methylglutaryl coenzyme A reductase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated 80S-ribosome + H2O
80S-ribosome + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated acetyl-CoA carboxylase + H2O
acetyl-CoA carboxylase + phosphate
show the reaction diagram
-
i.e. EC 6.4.1.2, phosphorylated
-
-
?
phosphorylated AD P-Tau + H2O
AD P-Tau + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Akt protein + H2O
Akt protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Akt protein
-
?
phosphorylated Akt1 + H2O
Akt1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated alpha-casein + H2O
alpha-casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated alpha-subunit of eukaryotic peptide initiation factor 2 + H2O
alpha-subunit of eukaryotic peptide initiation factor + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated alpha-subunit of succinyl-CoA synthetase + H2O
alpha-subunit of succinyl-CoA synthetase + phosphate
show the reaction diagram
-
activity is lower than towards phosphorylated nucleoside-diphosphate kinase
-
-
?
phosphorylated androgen receptor + H2O
androgen receptor + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of androgen receptor
-
?
phosphorylated angiomotin + H2O
angiomotin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated AP-1/AP-2 + H2O
AP-1/AP-2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of AP-1/AP-2
-
?
phosphorylated APC protein + H2O
APC protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of APC protein
-
?
phosphorylated apoptosis signal-regulating kinase 1 + H2O
apoptosis signal-regulating kinase 1 + phosphate
show the reaction diagram
P53041
apoptosis signal-regulating kinase 1 is transiently activated by autophosphorylation at Thr845
-
-
?
phosphorylated apoptosis signal-regulating kinase 1 + H2O
apoptosis signal-regulating kinase 1 + phosphate
show the reaction diagram
-
PGAM5 acts as a specific protein Ser/Thr phosphatase that activates apoptosis signal-regulating kinase 1 by dephosphorylation of inhibitory sites (phosphorylation of Thr-838), PGAM5 serves as a specialized activator of apoptosis signal-regulating kinase 1
-
-
?
phosphorylated ASK1 + H2O
ASK1 + phosphate
show the reaction diagram
-
ASK1 is inactivated by the isoform PP2Ceta
-
-
?
phosphorylated ataxia telangiectasia and Rad3-related + H2O
ataxia telangiectasia and Rad3-related + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ataxia telangiectasia mutated kinase + H2O
ataxia telangiectasia mutated kinase + phosphate
show the reaction diagram
P53041
-
-
-
?
phosphorylated ataxia telangiectasia mutated kinase + H2O
ataxia telangiectasia mutated kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ATM and Rad 3 related kinase + H2O
ATM and Rad 3 related kinase + phosphate
show the reaction diagram
P53041
-
-
-
?
phosphorylated ATM protein + H2O
ATM protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of ATM protein
-
?
phosphorylated atypical protein kinase C + H2O
atypical protein kinase C + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated aurora A + H2O
aurora A + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated aurora A + H2O
aurora A + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of aurora A
-
?
phosphorylated axin + H2O
axin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of axin
-
?
phosphorylated B-catenin + H2O
B-catenin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of B-catenin
-
?
phosphorylated BAD protein + H2O
BAD protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of BAD protein
-
?
phosphorylated BAX protein + H2O
BAX protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of BAX protein
-
?
phosphorylated BCKDH complex + H2O
BCKDH complex + phosphate
show the reaction diagram
-
i.e. branched-chain alpha-keto acid dehydrogenase, phosphorylated
-
-
?
phosphorylated BCL-2 + H2O
BCL-2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of BCL-2
-
?
phosphorylated Bcl2 + H2O
Bcl2 + phosphate
show the reaction diagram
-
PP2A-mediated dephosphorylation of Bcl2 promotes its direct interaction with p53 as well as a conformational change in Bcl2, PP2A directly interacts with the BH4 domain of Bcl2 as a docking site to potentially bridge PP2A to Bcl2's flexible loop domain containing the target serine 70 phosphorylation site
-
-
?
phosphorylated beta-catenin + H2O
beta-catenin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated brain proteins + H2O
brain proteins + phosphate
show the reaction diagram
-
e.g. DARPP-32, G-substrate, both phosphorylated at threonine, similar amino acid sequences and phosphorylation sites, protein K-F.
-
-
?
phosphorylated c-Jun N-terminal kinase + H2O
c-Jun N-terminal kinase + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
-
-
-
?
phosphorylated c-MET + H2O
c-MET + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of c-MET
-
?
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of c-Myc
-
?
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
-
PP2A plays an important role in control of proto-oncogene c-Myc by destabilization of c-Myc and promotion of its ubiquitin-mediated degradation, PP2a has tumor-suppressor activity, c-Myc regulation is essential for normal cell function, overview, dephosphorylation of Ser62, PP2A binds to c-Myc via targeting by regulatory subunit B56alpha, overview
-
-
?
phosphorylated Ca2+/calmodulin-dependent protein kinase II + H2O
Ca2+/calmodulin-dependent protein kinase II + phosphate
show the reaction diagram
-
dephosphorylation of the autophosphorylated Thr286, PPM1F negatively regulates the multifunctional Ca2+/calmodulin-dependent protein kinase II, e.g. reducing the phosphorylation of vimentin, dephosphorylation of the autophosphorylated Thr286 leading to CaMKII inactivation in vitro, PPM1F interacts with multifunctional Ca2+/calmodulin-dependent protein kinase II involving multiple regions independently of the phosphatase activity, analysis overview
-
-
?
phosphorylated CaBP4 + H2O
CaBP4 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated calmodulin-like domain of protein kinase + H2O
calmodulin-like domain of protein kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated calpain + H2O
calpain + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of calpain
-
?
phosphorylated CAM kinase IV + H2O
CAM kinase IV + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of CAM kinase IV
-
?
phosphorylated cAMP-dependent protein kinase + H2O
cAMP-dependent protein kinase + phosphate
show the reaction diagram
-
regulatory subunit
-
-
?
phosphorylated cAMP-dependent protein kinase + H2O
cAMP-dependent protein kinase + phosphate
show the reaction diagram
-
phosphorylated at Ser-95
-
-
?
phosphorylated carboxypeptidase D + H2O
carboxypeptidase D + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of carboxypeptidase D
-
?
phosphorylated CAS protein + H2O
CAS protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of CAS protein
-
?
phosphorylated casein (PKA) + H2O
casein (PKA) + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated casein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated casein + H2O
casein + phosphate
show the reaction diagram
P53042
-
-
-
?
phosphorylated casein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated casein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated casein + H2O
casein + phosphate
show the reaction diagram
Q84K11
-
-
-
?
phosphorylated Cav1.2 + H2O
Cav1.2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Cav1.2
-
?
phosphorylated Cav1.2 + H2O
Cav1.2 + phosphate
show the reaction diagram
Q28653
binding of PP2A to the L-type calcium channel Cav1.2 next to Ser1928, its main PKA site, is critical for Ser1928 dephosphorylation, binding of PP2A to the L-type calcium channel Cav1.2 next to Ser1928, its main PKA site, is critical for Ser1928 dephosphorylation, interaction study, overview
-
-
?
phosphorylated Cav1.2 + H2O
Cav1.2 + phosphate
show the reaction diagram
-
PP2A dephosphorylates Cav1.2 at serine 1866
-
-
?
phosphorylated Cbp1 + H2o
Cbp1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Cdc25c + H2O
Cdc25c + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Cdc25c
-
?
phosphorylated Cdc6 protein + H2O
Cdc6 protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of Cdc6 protein
-
?
phosphorylated Cdk1 + H2O
Cdk1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated CFTRCXCR2 + H2O
CFTRCXCR2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of CFTRCXCR2
-
?
phosphorylated chemotaxis protein CheA + H2O
chemotaxis protein CheA + phosphate
show the reaction diagram
-
activity is lower than towards phosphorylated nucleoside-diphosphate kinase
-
-
?
phosphorylated Chk1 + H2O
Chk1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Chk1 protein + H2O
Chk1 protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Chk1 protein
-
?
phosphorylated Chk2 + H2O
Chk2 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Chk2 + H2O
Chk2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Chk2
-
?
phosphorylated cyclin G1 + H2O
cyclin G1 + phosphate
show the reaction diagram
-
the B'alpha1 subunit of the serine/threonine protein phosphatase 2A, which binds to cyclin G1, can stabilize cyclin G1 under unstressed conditions and upon DNA damage, as well as inhibit the ability of cyclin G1 to be ubiquitinated
-
-
?
phosphorylated DARPP-32 + H2O
DARP-32 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of DARP-32
-
?
phosphorylated deoxycytidine kinase + H2O
deoxycytidine kinase + phosphate
show the reaction diagram
-
protein phosphatase 2A negatively regulates deoxycytidine kinase activity via Ser-74 dephosphorylation
-
-
?
phosphorylated DNA-dependent protein kinase + H2O
DNA-dependent protein kinase + phosphate
show the reaction diagram
-
activation of DNA-dependent protein kinase to repair DNA double strand breaks by the process of nonhomologous end joining
-
-
?
phosphorylated DNA-protease K + H2O
DNA-protease K + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated doublecortin + H2O
doublecortin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated E-cadherin + H2O
E-cadherin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of E-cadherin
-
?
phosphorylated Ebola virus VP30 protein + H2O
Ebola virus VP30 protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ERK + H2O
ERK + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
-
-
-
?
phosphorylated ERK + H2O
ERK + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
binds heat shock transcription factor 4 in a complex with ERK, controlling transcription factor DNA binding
-
-
?
phosphorylated ERK protein + H2O
ERK protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of ERK protein
-
?
phosphorylated ERK-2 + H2O
ERK-2 + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
-
-
-
?
phosphorylated ERK-2 + H2O
ERK-2 + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
preferred substrate
-
-
?
phosphorylated ERK1 + H2O
ERK1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ERK2 + H2O
ERK2 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ERK2 + H2O
ERK2 + phosphate
show the reaction diagram
-
PP2A acts as a gate-keeper of extracellular signal-regulated kinase, ERK, activity in neuronal PC12 cells, the PP2A regulatory subunits Balpha and Bdelta target the PP2A heterotrimer to dephosphorylate and inactivate ERK, silencing of B subunits leads to hyperactivation of ERK stimulated by constitutively active MEK1, overview, dephosphorylation of the activation loop threonine of ERK
-
-
?
phosphorylated ERK5 + H2O
ERK5 + phosphate
show the reaction diagram
-
dephosphorylation at Thr218 and Tyr220 residues, dephosphorylation site sequences, overview
-
-
?
phosphorylated estrogen receptor A + H2O
estrogen receptor A + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of estrogen receptor A
-
?
phosphorylated extracellular-signal-regulated kinase + H2O
extracellular-signal-regulated kinase + phosphate
show the reaction diagram
-
PP2A is involved in the inactivation, but not the activation, of extracellular-signal-regulated kinase
-
-
?
phosphorylated glucocorticoid receptor-heat shock protein 90 + H2O
glucocorticoid receptor-heat shock protein 90 + phosphate
show the reaction diagram
P53041
-
-
-
?
phosphorylated glycogen phosphorylase + H2O
glycogen phosphorylase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated glycogen phosphorylase a + H2O
glycogen phosphorylase a + phosphate
show the reaction diagram
-
inactivation of the substrate enzyme, overview
-
-
?
phosphorylated glycogen phosphorylase alpha + H2O
glycogen phosphorylase alpha + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Gp130 protein + H2O
Gp130 protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of Gp130 protein
-
?
phosphorylated GSK3beta + H2O
GSK3beta + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
laforin dephosphorylates the inhibitory Ser9 of GSK3beta
-
-
?
phosphorylated H2AX + H2O
H2AX + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of H2AX
-
?
phosphorylated H2AX + H2O
H2AX + phosphate
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae AY925
-
the Pph3p complex is required for dephosphorylation of H2AX, Pph21p (PP2Ac ortholog) is not involved in H2AX dephosphorylation
-
-
?
phosphorylated HAND-1 + H2O
HAND-1 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of HAND-1
-
?
phosphorylated HAND-2 + H2O
HAND-2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of HAND-2
-
?
phosphorylated HDAC4 + H2O
HDAC4 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of HDAC4
-
?
phosphorylated High mobility group box 1 protein + H2O
High mobility group box 1 protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone + H2O
histone + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone + H2O
histone + phosphate
show the reaction diagram
P48452
-
-
-
?
phosphorylated histone 2A member X + H2O
histone 2A member X + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone deacetylase 3 + H2O
histone deacetylase 3 + phosphate
show the reaction diagram
-
dephosphorylation at Ser424, PP4 negatively regulates the activity of histone deacetylase 3 by direct protein-protein interaction also involving the so-repressors N-CoR and SMRT, PP4 regulates histone deacetylase 3, HDAC3, activity by dephosphorylation of Ser424, and by direct protein-protein interaction of active PP4 which is complexed with HDAC3 via the N-terminus of the deacetylase, PP4 is the limiting factor and is not saturating in the complex in vivo, i.e. HDAC3, a class I histone deacetylase, dephosphorylation at Ser424 of the protein kinase CK2 phosphoacceptor site, PP4 dephosphorylates Ser424
-
-
?
phosphorylated histone deacetylase 7 + H2O
histone deacetylase 7 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone h1 (PKA) + H2O
histone h1 (PKA) + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone h1 (PKC) + H2O
histone h1 (PKC) + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone H3 + H2O
histone H3 + phosphate
show the reaction diagram
-
dephosphorylation of histone H3 at Ser10 by PP1 is required for DNA methylation of some loci
-
-
?
phosphorylated histone IIA + H2O
histone IIA + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated hormone-sensitive lipase + H2O
hormone-sensitive lipase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Hsp90 + H2O
Hsp90 + phosphate
show the reaction diagram
-
PP5 positively regulates the function of Hsp90 to maintain cellular homeostasis during proteotoxic stresses
-
-
?
phosphorylated IkappaB kinase + H2O
IkappaB kinase + phosphate
show the reaction diagram
-
PP2A positively regulates multicomponent Ikappa kinase signaling, eventhough it inhibits its activity by dephosphorylation, positive regulation requires PP2A-IKK complex formation, suppression of the complex formation attenuates IKK T loop phosphorylation and activation, PP2A inhibition attenuates TNFalpha-induced IKK degradation, overview, formation of PP2A-IKK complexes, determination of interaction sites
-
-
?
phosphorylated IkappaB kinase + H2O
IkappaB kinase + phosphate
show the reaction diagram
Mus musculus C57BL6
-
PP2A positively regulates multicomponent Ikappa kinase signaling, eventhough it inhibits its activity by dephosphorylation, positive regulation requires PP2A-IKK complex formation, suppression of the complex formation attenuates IKK T loop phosphorylation and activation, PP2A inhibition attenuates TNFalpha-induced IKK degradation, overview, formation of PP2A-IKK complexes, determination of interaction sites
-
-
?
phosphorylated IKKB + H2O
IKKB + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of IKKB
-
?
phosphorylated IKKB protein + H2O
IKKB protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of IKKB protein
-
?
phosphorylated ILK1 + H2O
ILK1 + phosphate
show the reaction diagram
-
negative regulation of Wnt signaling, Wip1
-
-
?
phosphorylated INAD-protein + H2O
INAD-protein + phosphate
show the reaction diagram
-
INAD-protein is inactivation no afterpotential D-protein
-
-
?
phosphorylated inhibitor-1 + H2O
inhibitor-1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated inhibitor-2 + H2O
inhibitor-2 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated JNK protein + H2O
JNK protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated JNK protein + H2O
JNK protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of JNK protein
-
?
phosphorylated JNK1 + H2O
JNK1 + phosphate
show the reaction diagram
-
dephosphorylation at Thr183 and Tyr185 residues, dephosphorylation site sequences, overview
-
-
?
phosphorylated katanin p60 + H2O
katanin p60 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated keratin 8 + H2O
keratin 8 + phosphate
show the reaction diagram
-
dephosphorylates at phospho-Ser431
-
-
?
phosphorylated KSR protein + H2O
KSR protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of KSR protein
-
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
-
the substrate enzyme regulates the activity of the Ca2+-ATPase pump on the sarcoplasmic reticulum and is a key regulator of both basal contractility and the heart's beta-agonist responses, overview
dephosphorylated phospholamban exhibits an inhibitory effect on SERCA2a
-
?
phosphorylated Mdm2 protein + H2O
Mdm2 protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of Mdm2 protein
-
?
phosphorylated MEK3 + H2O
MEK3 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of MEK3
-
?
phosphorylated MEKK3 + H2O
MEKK3 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of MEKK3
-
?
phosphorylated messenger ribonucleoprotein + H2O
messenger ribonucleoprotein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated microtubule associated protein + H2O
microtubule associated protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Mid1 + H2O
Mid1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Mih1 + H2O
Mih1 + phosphate
show the reaction diagram
-
protein phosphatase 2A associated with Cdc55 dephosphorylates Mih1
-
-
?
phosphorylated mitogen-activated protein kinase kinase 3 + H2O
mitogen-activated protein kinase kinase 3 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated mitogen-activated protein kinase Pmk1p + H2O
mitogen-activated protein kinase Pmk1p + phosphate
show the reaction diagram
Schizosaccharomyces pombe, Schizosaccharomyces pombe MI200
-
-
-
-
?
phosphorylated MKK3b + H2O
MKK3b + phosphate
show the reaction diagram
-
MKK3b is inactivated by the isoforms PP2Calpha and PP2Cbeta
-
-
?
phosphorylated MKK4 + H2O
MKK4 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of MKK4
-
?
phosphorylated MKK4 + H2O
MKK4 + phosphate
show the reaction diagram
-
MKK4 is inactivated by the isoforms PP2Calpha and PP2Cbeta
-
-
?
phosphorylated MKK6b + H2O
MKK6b + phosphate
show the reaction diagram
-
MKK6b is inactivated by the isoforms PP2Calpha and PP2Cbeta
-
-
?
phosphorylated MKK7 + H2O
MKK7 + phosphate
show the reaction diagram
-
MKK7 is inactivated by the isoforms PP2Calpha and PP2Cbeta
-
-
?
phosphorylated mouse double minute 2 homologue + H2O
mouse double minute 2 homologue + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated myelin basic protein + H2O
?
show the reaction diagram
-
-
-
-
?
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
dephosphorylation at Thr696
-
-
?
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
isozyme PP1delta regulates smooth muscle relaxation
-
-
?
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
isozyme PP1delta, the reaction involves the RVxF-containing myosin phosphatase targeting subunit 1, MYPT1
-
-
?
phosphorylated myosin light chain 2 + H2O
myosin light chain 2 + phosphate
show the reaction diagram
-
preferred target substrate for PP1 in the thick filament
-
-
?
phosphorylated myosin light chain peptide + H2O
myosin light chain peptide + phosphate
show the reaction diagram
-
KAKTTKKRPQRATSNVFS
-
-
?
phosphorylated NDEL1 + H2O
NDEL1 + phosphate
show the reaction diagram
-
PP4c selectively dephosphorylates NDEL1 at T219
-
-
?
phosphorylated nemo protein + H2O
nemo protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of nemo protein
-
?
phosphorylated nucleoside-diphosphate kinase + H2O
nucleoside-diphosphate kinase + phosphate
show the reaction diagram
-
dephosphorylation of nucleoside-diphosphate kinase leads to loss of enzymatic activity, an efficient mode of posttranslational modification of the enzyme crucial to cellular development. Decreasing order of efficiency: nucleoside-diphosphate kinase from Homo sapiens, nucleoside-diphosphate kinase from Pseudomonas aeruginosa, nucleoside-diphosphate kinase from Escherichia coli, nucleoside-diphosphate kinase from yeast
-
-
?
phosphorylated nucleotide diphosphate kinase 2 + H2O
nucleotide diphosphate kinase 2 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated occludin + H2O
occludin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of occludin
-
?
phosphorylated P107 + H2O
P107 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of P107
-
?
phosphorylated p38 + H2O
p38 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated p38 + H2O
p38 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated p38 + H2O
p38 + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
-
-
-
?
phosphorylated p38alpha + H2O
p38alpha + phosphate
show the reaction diagram
-
mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway, substrate is a MAPK, identification of dephosphorylation at Thr residues, dephosphorylation site sequences, activity with wild-type and mutant p38alpha proteins, overview
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
dephosphorylation of Ser15 by PP1, no activity with PP2A, PP1 inhibition leads to hyperphosphorylation of p53, PP1 has regulatory function
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
the B56-containing PP2A acts partly through p53 suppressing apoptosis
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
dephosphorylation of Ser15 by PP1, no activity with PP2A
-
-
?
phosphorylated p53 protein + H2O
p53 protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of p53 protein
-
?
phosphorylated p53-binding protein 1 + H2O
p53-binding protein 1 + phosphate
show the reaction diagram
-
p53-binding protein 1 is dephosphorylated at Ser25 and Ser1778
-
-
?
phosphorylated PACS-1 + H2O
PACS-1 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of PACS-1
-
?
phosphorylated Par-3 + H2O
Par-3 + phosphate
show the reaction diagram
-
predominantly the alpha-isoform of PP1 binds phosphorylated Par-3 and specifically dephosphorylates at Ser-144 and Ser-824
-
-
?
phosphorylated Par-6 + H2O
Par-6 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated paxillin + H2O
paxillin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of paxillin
-
?
phosphorylated penicillin-binding protein + H2O
penicillin-binding protein + phosphate
show the reaction diagram
Enterococcus faecium, Enterococcus faecium D344S
-
-
-
-
?
phosphorylated peptide + H2O
peptide + phosphate
show the reaction diagram
-
PP2Cdelta prefers the dephosphorylation at diphosphorylated T(P)X(P)Y sequence compared to monophosphorylated T(P)XY sequence
-
-
?
phosphorylated period + H2O
period + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of period
-
?
phosphorylated phosphoenolpyruvate carboxylase + H2O
phosphoenolpyruvate carboxylase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase a + H2O
phosphorylase a + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase a + H2O
phosphorylase a + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase a + H2O
phosphorylase a + phosphate
show the reaction diagram
P61292
-
-
-
?
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
O15757
-
-
-
?
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
Q9U493
-
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
isozyme PP1 preferentially dephosphorylates the beta-subunit of phosphorylase kinase, isozyme PP2 preferentially dephosphorylates the alpha-subunit of phosphorylase kinase
-
-
?
phosphorylated phytochrome A + H2O
phytochrome A + phosphate
show the reaction diagram
-
PAPP2C dephosphorylates both native oat phytochrome A and a point mutant of S598A, whereas an N-terminal deletion of 65 amino acids prevents dephosphorylation
-
-
?
phosphorylated phytochrome B + H2O
phytochrome B + phosphate
show the reaction diagram
-
phytochrome B is autophosphorylated, the N-terminal extension-deleted phytochrome B shows no apparent autophosphorylation activity
-
-
?
phosphorylated PIM protein + H2O
PIM protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of PIM protein
-
?
phosphorylated PKCbeta II + H2O
PKBbeta II + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of PKCbeta II
-
?
phosphorylated PKCdelta + H2O
PKCdelta + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of PKCdelta
-
?
phosphorylated Polo-like kinase 1 + H2O
Polo-like kinase 1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated pRb protein + H2O
pRb protein + phosphate
show the reaction diagram
-
ceramide prevents retinoblastoma gene product pRb protein hyperphosphorylation by PP1 activation, nerve growth factor deprivation in sympathetic neurons leads to inhibition of PP1 and hyperphosphorylation of pRb protein
-
-
?
phosphorylated protein kinase CK2alpha + H2O
protein kinase CK2alpha + phosphate
show the reaction diagram
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
-
-
-
?
phosphorylated protein kinase Hs11 + H2O
protein kinase Hs11 + phosphate
show the reaction diagram
-
contributes to its delocalization and destruction, at high Ca2+ concentrations
-
-
?
phosphorylated protein phosphatase inhibitor-1 + H2O
protein phosphatase inhibitor-1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated protein phosphatase inhibitor-1 + H2O
protein phosphatase inhibitor-1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Prz1 + H2o
Prz1 + phosphate
show the reaction diagram
-
induction of calcineurin itself and the Pmc1 CXa2+ ATPase
-
-
?
phosphorylated pyruvate dehydrogenase + H2O
pyruvate dehydrogenase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated pyruvate dehydrogenase + H2O
pyruvate dehydrogenase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Raf + H2O
Raf + phosphate
show the reaction diagram
-
Raf is dephosphorylated at Ser259
-
-
?
phosphorylated Raf-1 kinase + H2O
Raf 1 kinase + phosphate
show the reaction diagram
-
phosphatase 2A dephosphorylates Raf-1 Ser259 in response to mitogens
-
-
?
phosphorylated Raf-1 kinase + H2O
Raf 1 kinase + phosphate
show the reaction diagram
-
activation of Raf-1 by dephopshorylation at Ser259
-
-
?
phosphorylated Raf1 + H2O
Raf-1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Raf1 + H2O
Raf1 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of Raf1
-
?
phosphorylated RalA + H2O
RalA + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of RalA
-
?
phosphorylated RelA + H2O
RelA + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of RelA
-
?
phosphorylated retinoblastoma protein + H2O
retinoblastoma protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated rhodopsin + H2O
rhodopsin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated RNA polymerase II + H2O
RNA polymerase II + phosphate
show the reaction diagram
-
dephosphorylation of the carboxyterminal domain
-
-
?
phosphorylated SCR protein + H2O
SCR protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of SCR protein
-
?
phosphorylated securin + H2O
securin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated securin + H2O
securin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of securin
-
?
phosphorylated Ser/Thr protein kinase + H2O
DNA-dependent Ser/Thr protein kinase + phosphate
show the reaction diagram
P53041
-
-
-
?
phosphorylated seratonin + H2O
seratonin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of seratonin
-
?
phosphorylated serine-phosphorylated glycogen phosphorylase + H2O
glycogen phosphorylase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated serine/threonine protein kinase + H2O
serine/threonine protein kinase + phosphate
show the reaction diagram
Enterococcus faecium, Enterococcus faecium D344S
-
-
-
-
?
phosphorylated Shc protein + H2O
Shc protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Shc protein
-
?
phosphorylated Sp1 + H2O
Sp1 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of Sp1
-
?
phosphorylated spliceosome-associated protein 155 + H2O
spliceosome-associated protein 155 + phosphate
show the reaction diagram
-
i.e. Sap155, a U2 small nuclear ribonucleoprotein particle component. nuclear inhibitor of PP1, i.e. NIPP1, a major PP1 interactor in the vertebrate nucleus, recruits PP1 to Sap155, i.e. Sap155, a U2 small nuclear ribonucleoprotein particle component
-
-
?
phosphorylated sprouty + H2O
sprouty + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of sprouty
-
?
phosphorylated Src protein + H2O
Src protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of Src protein
-
?
phosphorylated stress-activated protein kinase + H2O
stress-activated protein kinase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated TAK1-binding protein 1 + H2O
TAK1-binding protein 1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Tau + H2O
Tau + phosphate
show the reaction diagram
-
the Balpha regulatory subunit of PP2A facilitates dephosphorylation of the phosphorylated Tau
-
-
?
phosphorylated tau protein + H2O
tau protein + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of tau protein
-
?
phosphorylated tau-factor + H2O
tau-factor + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Tcn1/Crz1 + H2O
Tcn1/Crz1 + phosphate
show the reaction diagram
-
promotes its translocation into the nucleus and exposure of a transcription activation domain
-
-
?
phosphorylated threonine-phosphorylated lipocortin I + H2O
lipocortin I + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated TRAF2 + H2O
TRAF2 + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to inhibition of TRAF2
-
?
phosphorylated transforming growth factor beta-activated kinase 1 + H2O
transforming growth factor beta-activated kinase 1 + phosphate
show the reaction diagram
-
dephosphorylates Thr-187 in the TAK1 activation loop
-
-
?
phosphorylated transforming growth factor-beta-activated kinase 1 + H2O
transforming growth factor-beta-activated kinase 1 + phosphate
show the reaction diagram
-
Thr187 in transforming growth factor-beta-activated kinase 1 is a major dephosphorylation target of PP2A, PP2A plays a pivotal role as a negative regulator of transforming growth factor-beta-activated kinase 1 activation in response to transforming growth factor-beta 1
-
-
?
phosphorylated transforming growth factor-beta-activated kinase 1 + H2O
transforming growth factor-beta-activated kinase 1 + phosphate
show the reaction diagram
-
transforming growth factor-beta-activated kinase 1 is inactivated by the isoforms PP2Cbeta and PP2Cepsilon
-
-
?
phosphorylated tropomyosin-related kinase A + H2O
tropomyosin-related kinase A + phosphate
show the reaction diagram
-
two neuron-enriched regulatory subunits, B'beta and B'delta, recruit PP2A into a complex with tropomyosin-related kinase A to dephosphorylate the nerve growth factor receptor on Ser/Thr residues and to potentiate its intrinsic tyrosine kinase activity
-
-
?
phosphorylated troponin + H2O
troponin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated troponin I + H2O
troponin I + phosphate
show the reaction diagram
-
preferred target substrate for PP1 in the thin filament
-
-
?
phosphorylated troponin T + H2O
troponin T + phosphate
show the reaction diagram
-
preferred target substrate for PP1 in the thin filament
-
-
?
phosphorylated tumor suppressor protein p53 + H2O
tumor suppressor protein p53 + phosphate
show the reaction diagram
-
dephosphorylation at Thr55
-
-
?
phosphorylated UNG2 + H2O
UNG2 + phosphate
show the reaction diagram
-
dephosphorylation at Thr residues, identification of dephosphorylation site sequences, overview
-
-
?
phosphorylated vimentin + H2O
vimentin + phosphate
show the reaction diagram
-
-
the dephosphorylation leads to activation of vimentin
-
?
phosphoserine-casein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoserine-myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoserine-RCML + H2O
RCML + phosphate
show the reaction diagram
-
i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphoserine
-
-
?
phosphospectrin + H2O
spectrin + phosphate
show the reaction diagram
-
-
-
-
?
phosphothreonine + H2O
threonine + phosphate
show the reaction diagram
Q8N3J5
-
-
-
?
phosphotyrosine-casein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphotyrosine-myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphotyrosine-RCML + H2O
RCML + phosphate
show the reaction diagram
-
i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphotyrosine
-
-
?
PIPGRFDRRVS(P)VAAE + H2O
PIPGRFDRRVSVAAE + phosphate
show the reaction diagram
-
-
-
-
?
Rad53p + H2O
?
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae AY925
-
a complex of Pph3p and Psy2p may dephosphorylate Rad53p after it has been activated and phosphorylated in response to methylmethanesulfonate, but a cisplatin-induced activation and phosphorylation of Rad53p may require a complex of Pph3, Psy4p and Psy2p for dephosphorylation
-
-
?
Raf proto-oncogene serine/threonine protein kinase + H2O
dephosporylated Raf proto-oncogene serine/threonine protein kinase + phosphate
show the reaction diagram
P53041
PP5 plays a role in the inactivation of Raf proto-oncogene serine/threonine protein kinase, PP5/Raf proto-oncogene serine/threonine protein kinase association occurs in response to growth factor stimulation and results in the selective dephosphorylation of Ser338, removing phosphate from this critical site that helps to maintain Raf proto-oncogene serine/threonine protein kinase activation
-
-
?
RAT(P)VA + H2O
RATVA + phosphate
show the reaction diagram
-
-
-
-
?
RII peptide + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
RRA(pT)VA + H2O
RRATVA + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
RRA(pT)VA + H2O
RRATVA + phosphate
show the reaction diagram
Sulfolobus acidocaldarius MW001
Q4JAD1, Q4JB88
-
-
-
?
RRApTVA + H2O
RRATVA + phosphate
show the reaction diagram
-
-
-
-
?
RRApTVA + H2O
RRATVA + phosphate
show the reaction diagram
-
-
-
-
?
RRApTVA + H2O
RRATVA + phosphate
show the reaction diagram
-
-
-
-
?
RRApTVA + H2O
RRATVA + phosphate
show the reaction diagram
-
a protein concentration at 15 ng per reaction was found to be optimal for dephosphorylation of the synthetic peptide RRApTVA, maximum enzyme activity can be achieved in the presence of 100 ng of substrate
-
-
?
RRApTVA + H2O
RRATVA + phosphate
show the reaction diagram
chicken anemia virus VP2
-
-
-
-
?
RRAS(P)VA + H2O
RRASVA + phosphate
show the reaction diagram
-
synthetic substrate
-
-
?
RRAS(P)VA + H2O
RRASVA + phosphate
show the reaction diagram
-
synthetic substrate
-
-
?
RRAT(P)VA + H2O
RRATVA + phosphate
show the reaction diagram
-
synthetic substrate
-
-
?
RRAT(P)VA + H2O
RRATVA + phosphate
show the reaction diagram
-
synthetic substrate
-
-
?
RRLIEDAEY(P)AARG + H2O
RRLIEDAEYAARG + phosphate
show the reaction diagram
-
synthetic substrate
-
-
?
RRREEET(P)EE + H2O
RRREEETEE + phosphate
show the reaction diagram
-
-
-
-
?
serine/threonine-phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
SSpSLESLQTAVAEVTLNGNIPFHRPR + H2O
SSSLESLQTAVAEVTLNGNIPFHRPR + phosphate
show the reaction diagram
-
-
-
-
?
synthetic peptides with phosphotyrosyl residues + H2O
synthetic peptides + phosphate
show the reaction diagram
-
-
-
-
?
synthetic peptides with phosphotyrosyl residues + H2O
synthetic peptides + phosphate
show the reaction diagram
-
selectivity toward phosphopeptide substrates
-
-
?
tetrafluorotyrosine phosphate + H2O
tetrafluorotyrosine + phosphate
show the reaction diagram
-
-
-
-
?
tumor necrosis factor receptor-associated factor 6 + H2O
?
show the reaction diagram
-
PP4 negatively regulates lipopolysaccharide-induced and tumor necrosis factor receptor-associated factor 6-mediated nuclear factor-kappaB activation by inhibiting the ubiquitination of tumor necrosis factor receptor-associated factor 6, PP4 is a negative feedback regulator of lipopolysaccharide/toll-like receptor 4 pathway
-
-
?
tyrosine-phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
-
-
-
?
tyrosine-phosphorylated sEGF-R + H2O
phosphorylated sEGF-R + phosphate
show the reaction diagram
-
-
-
-
?
UNC-89 + H2O
?
show the reaction diagram
-
the protein kinase 2 region of UNC-89 specifically interacts with SCPL-1
-
-
?
vasodilator associated phosphoprotein + H2O
vasodilator associated protein + phosphate
show the reaction diagram
-
-
-
-
?
[a peptide]-serine/threonine phosphate + H2O
[a peptide]-serine/threonine + phosphate
show the reaction diagram
Pyrodictium abyssi, Pyrodictium abyssi TAG11
O08367
-
-
-
?
[protein]-L-serine phosphate + H2O
[protein]-L-serine + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
[protein]-L-serine phosphate + H2O
[protein]-L-serine + phosphate
show the reaction diagram
-
the enzyme dephosphorylates phosphoseryl residues in casein, mixed histones, and phosphorylase a, but not phosphotyrosyl residues in reduced, carboxyamidomethylated and maleylated lysozyme
-
-
?
[protein]-L-serine phosphate + H2O
[protein]-L-serine + phosphate
show the reaction diagram
Sulfolobus acidocaldarius MW001
Q4JAD1, Q4JB88
-
-
-
?
[protein]-L-threonine phosphate + H2O
[protein]-L-threonine + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
KRpTIRR + H2O
KRTIRR phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5
-
-
-
additional information
?
-
-
no activity with p-nitrophenyl phosphate
-
-
-
additional information
?
-
-
ceramide-induced cell survival mechanism, overview
-
-
-
additional information
?
-
A2XN40, A3C4N5, Q9LKF8
in vivo expression and response pattern of PP2A gene to environmental stress
-
-
-
additional information
?
-
-
PP1 contains a surface hydrophobic pocket on the catalytic subunit which is important in recognizing cellular regulators via their consensus RVxF motifs, regulation of the enzyme, overview
-
-
-
additional information
?
-
-
PP2A is a multifunctional enzyme, direct interaction with the adenovirus E4orf4 protein via the regulatory subunit initiation virus-induced growth arrest in yeast cells, signal transduction is mediated by Cdc55 interacting with both PP2A catalytic subunit in absence of the Tpd3 subunit and E4orf4, mechanism involving the Tpd3 yeast subunit, overview
-
-
-
additional information
?
-
-
PP2A is involved in central sensitization, PP2A negatively regulates the phosphorylation of N-methyl-D-aspartate, NMDA, receptors in response to capsaicin induction in the spinal cord
-
-
-
additional information
?
-
-
PP2A is involved in the maintenance of E-cadherin-mediated cell-cell adhesion through recruitment of IQGAP1, an effector of small GTPase Rac
-
-
-
additional information
?
-
-
PP2A modulates Akt activity, role of PP2A holoenzyme and subunits in MAPK signaling pathways, overview
-
-
-
additional information
?
-
-
PP2A regulates a wide variety of cellular signal transduction pathways, and performs positive regulation of Raf1-MEK1/2-ERK1/2 signaling at multiple steps of the pathway, overview
-
-
-
additional information
?
-
-
PP4 activates c-Jun N-terminal kinase JNK-1 and is required for JNK-1 stability in prostate cancer cells, PP4 is involved in the MAPK signaling pathway, overview
-
-
-
additional information
?
-
-
PP4 is involved in TNFalpha signalling, PP4 down-regulates IRS-4 and mediates TNFalpha-induced IRS-4 degradation, overview
-
-
-
additional information
?
-
-
PP5 acts as an effector in Rac GTPase signalling via interaction of its N-terminal TPR domain with Rac-GTP, PP5 inhibition results in blockage of KCNH2 channel stimulation by thyroid hormone and by Rac in pituitary cells, overview
-
-
-
additional information
?
-
P36873
PP5 affects many signaling pathways regulating cell growth and cellular stress response
-
-
-
additional information
?
-
-
PP5 is involved in MAPK signal pathways, PP5 suppresses the hypoxia-induced ASK-1/MKK-4/JNK signaling via two mechanisms, 1. by association to ASK-1, 2. by suppressing the phosphorylation of MKK-4, JNK, and c-Jun, overview
-
-
-
additional information
?
-
-
Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5
-
-
-
additional information
?
-
-
regulation and function of the stably associated calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex, PP2A is negatively regulating the kinase, interaction requires the Ca2+/CaM-binding autoinhibitory domain of CaMKIV
-
-
-
additional information
?
-
-
the cystic fibrosis transmembrane conductance regulator, a cAMP-activated chloride channel at the apical surface of epithelia, is regulated by a direct interaction with the protein phosphatase 2A
-
-
-
additional information
?
-
Q96LZ3
the enzyme is critical in many calcium-dependent signaling transduction pathways
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including circadian rhythm and apoptosis, overview
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including circadian rhythm, moluating the circadian transcription factor FRQ, and apoptosis, overview
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including embryonic development, cell proliferation, cell death, and cancer, overview, PP5 is involved in stress response negatively regulating ASK-1 dependent apoptosis and promoting tumor growth
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including embryonic development, cell proliferation, cell death, and cancer, overview, PP5 is involved in stress response negatively regulating ASK-1 dependent apoptosis and promoting tumor growth, PP2A is regulated by the small t antigen of SV40 and polyomavirus, and is involved in targeting of nuclear transcription factors, e.g. HAND1 and HAND2, overview
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including embryonic development, cell proliferation, cell death, circadian rhythm and cancer, overview, knockout of the PP2A regulator alpha4 causes p53-regulated apoptosis correlated with increased phosphorylation of p53 and JNK, PP2A is involved in regulation of PERIOD degradation and de/phosphorylation important in cricadian rhythm, overview
-
-
-
additional information
?
-
P62207
the enzyme is involved in regulation of many biological processes, e.g. glycogen metabolism, cell-cycle progression, and muscle relaxation, regulatory mechanism involving myosin phosphatase targeting subunit MYPT1 in smooth muscle relaxation, overview
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome and regulation of chromatin activities, it is involved in spliceosomal assembly via interaction with the survival of motor neuron complexes, PP4 regulates several cellular signalling routes, including the NF-kappaB and the target of rapamycin pathways, PP4 plays a role in DNA damage response and decrease histone deacetylase activity, phylogenetic tree and PPP phosphome, overview
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome, overview, PP4 depletion results in disrupted centrosome maturation and a decreased number of chiasmata between homologous chromosomes during meiosis in oocytes
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome, phylogenetic tree and PPP phosphome, overviewe
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome, PP4 homologues participate in the nutrient sensing pathway, pathway modeling overview, and confers resistance to the anticancer DNA-binding agents cisplatin and oxaliplatin, phylogenetic tree and PPP phosphome, overview
-
-
-
additional information
?
-
-
the Pro33 isoform of integrin beta3 enhances outside-in signaling in platelets by regulating the activation of serine/threonine phosphatases
-
-
-
additional information
?
-
-
direct interaction between the N terminus of Rattus norvegicus adenylyl cyclase AC8 and the active catalytic subunit of PP2A activates the dephosphorylation activity
-
-
-
additional information
?
-
P62207
myosin phosphatase targeting subunit MYPT1 increases myosine specificity of PP1, interaction and binding structure, overview
-
-
-
additional information
?
-
-
PP2A is a multifunctional enzyme, direct interaction with the adenovirus E4orf4 protein via the regulatory subunit
-
-
-
additional information
?
-
-
PP4 interacts with the TNFalpha-inducible insulin receptor substrate-4, IRS-4, the interaction is increased after TNFalpha-stimulation
-
-
-
additional information
?
-
-
substrate specificity of isozyme PP2Cdelta, overview
-
-
-
additional information
?
-
-
substrate specificity of PP2A isozymes
-
-
-
additional information
?
-
-
the different serine/threonine protein phosphatases are associated to a variety of other proteins, e.g. PP1 has about 50 associated proteins, PP5 contains an extended N-terminal domain with three tetratricopeptide repeat, TPR, motifs which possesses autoinhibitory function and are important for interaction with other proteins
-
-
-
additional information
?
-
P36873
the enzyme performs metal-mediated substrate hydrolysis
-
-
-
additional information
?
-
-
the enzyme preforms dephosphorylation of phosphoserine, phosphothreonine, and also phosphotyrosine residues
-
-
-
additional information
?
-
-
dephosphorylates protein kinase C alpha/betaII on threonine 638/641
-
-
-
additional information
?
-
-
dephosphorylates the endotoxic lipopolysaccharide of Escherichia coli 055B5
-
-
-
additional information
?
-
-
no activity on phosphoserine substrates
-
-
-
additional information
?
-
-
phosphothreonine-containing peptides with the sequence pTXpY are physiological substrates
-
-
-
additional information
?
-
-
PP2A potently dephosphorylates the mitotic protein monoclonal 2 site on topoisomerase II alpha
-
-
-
additional information
?
-
-
PP2A does not modify the activity of no receptor potential A-protein
-
-
-
additional information
?
-
-
PP2Ac associates with the integrin alphaIIbbeta3 complex in platelets, the alphaIIb membrane proximal region containing the KVGFFKR sequence can support the direct interaction of PP2Ac
-
-
-
additional information
?
-
P53041
PP5 binds the A-subunit of the PP2A holoenzyme, which functions to tether the catalytic (PP2Ac) and substrate targeting B-subunits of PP2A, PP5 is a potentially important regulator of both hormone- and stress-induced signaling networks that enable a cell to respond appropriately to genomic stress
-
-
-
additional information
?
-
-
the enzyme is involved in bacterial swarming activity and biofilm formation, PA3346 is the only of seven orphan response regulators inthe organism that is trans-phosphorylated by HptB
-
-
-
additional information
?
-
-
the expression of wild type PP2A catalytic subunit suppresses the MAPK kinase 3 phosphorylation
-
-
-
additional information
?
-
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
laforin shows diminished in vitro dephosphorylation of p-nitrophenyl phosphate in the presence of glucose polymers
-
-
-
additional information
?
-
-
little activity is observed with 4-nitrophenyl sulfate and 4-nitrophenyl thiophosphate
-
-
-
additional information
?
-
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
malin is an E3 ubiquitin ligase that promotes the degradation of laforin
-
-
-
additional information
?
-
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
MKP-5 does not dephosphorylate ERK
-
-
-
additional information
?
-
-
PAPP2C indirectly mediates the dephosphorylation of phytochrome-interacting factor 3 via the dephosphorylation of phytochromes
-
-
-
additional information
?
-
-
PP2A dephosphorylates all four 14-3-3 binding sites in the class IIa histone deacetylase 7, PP2A constitutively dephosphorylates histone deacetylase 7 to control its biological functions as a regulator of T cell apoptosis and endothelial cell functions
-
-
-
additional information
?
-
P49597, Q9FIF5
different mutants: mutations in genes involved in the ethylene signal transduction pathway and a mutation at the start of exon 2
-
-
-
additional information
?
-
-
GST-tagged PGAM5 does not dephosphorylate phospho-Tyr peptides (like END-pYINASL or DADEpYLIPQQG)
-
-
-
additional information
?
-
-
calcineurin is a Ca2+/calmodulin-dependent protein phosphatase
-
-
-
additional information
?
-
-
physical and functional interaction of the phosphatase calcineurin with a group of modulators, the RCAN protein family, e.g. proteins Cabin1 and AKAP79, a valine-rich region within the RCAN carboxyl region is essential for binding to the docking site in calcineurin, overview. Competition by substrates and modulators for a common docking site appears to be an essential mechanism in the regulation of Ca2+-calcineurin signaling
-
-
-
additional information
?
-
-
regulation of cellular PP1 by phosphorylation of the CPI-17 family, C-kinase-activated PP1 inhibitors, overview
-
-
-
additional information
?
-
-
the enzyme is cleaved by caspase-3, PP1 inhibitor Inh3 harbors a putative caspase-3 cleavage site, 46DTVD49, located between the two PP1 interaction sites, which consist of a RVXF motif and an adjacent inhibitory domain, both of which are required for inhibition of PP1
-
-
-
additional information
?
-
-
HLH-11, a member of basic helix-loop-helix proteins, binds to calcineurin catalytic subunit Tax-6, probably regulating TAX-6 as a transcription factor and/or a functional regulator. HLH-11 is involved in regulation of many developmental processes such as cell proliferation and differentiation, sex determination and myogenesis, HLH-11 deletion phenotype, overview
-
-
-
additional information
?
-
P48452
residues at 97-201 are essential for bovine retina CaN subunit A phosphatase activity
-
-
-
additional information
?
-
Q4JAD1, Q4JB88
no activity with TEVGKRI(pY)RLVGDKN
-
-
-
additional information
?
-
-
kinesin-8 KIF18A interacts with protein phosphatase 1
-
-
-
additional information
?
-
Sulfolobus acidocaldarius MW001
Q4JAD1, Q4JB88
no activity with TEVGKRI(pY)RLVGDKN
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
myosin light chain phosphate + H2O
myosin light chain + phosphate
show the reaction diagram
-
myosin light chain phosphatase is a specific form of PP1
-
-
?
phospho-Cdk2 + H2O
Cdk6 + phosphate
show the reaction diagram
-
-
-
-
?
phospho-Cdk6 + H2O
Cdk6 + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-serine + H2O
L-serine + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-threonine + H2O
L-threonine + phosphate
show the reaction diagram
-
-
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
show the reaction diagram
-
preferred substrate
-
-
?
phospho-MKK + H2O
MKK + phosphate
show the reaction diagram
-
-
-
-
?
phospho-PA3347 + H2O
PA3347 + phosphate
show the reaction diagram
-
dephosphorylation of Ser56 by PA3346
-
-
?
phospho-TAK1 + H2O
TAK1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
Q55059
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P36873, P62136, P62140
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P48452
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P36873
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
Q4QE27
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
P67775
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
U5PYV4
-
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
role of phosphatases in smooth muscle contractile systems
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
involved in the intracellular mechanism through which adrenocortical steroidogenesis is regulated, acting at a point after cAMP generation and action but proximal to the side-chain cleavage of cholesterol
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
modulation of pyruvate dehydrogenase complex
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
phosphatase PP3 stimulates the initiation of DNA-synthesis
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
involved in regulating exocytosis from the rat parotid gland via a cAMP-mediated process
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
control of cholesterol genesis
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
role in cAMP catabolism
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
capable of dephosphorylating the major proteins involved in control of glycogen metabolism, glycolysis, gluconeogenesis, aromatic amino acid breakdown, fatty acid, cholesterol, and protein synthesis
-
-
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphopyruvate kinase + H2O
pyruvate kinase + phosphate
show the reaction diagram
-
regulation of protein kinase during the transition to anoxia and back to the normoxic state
-
-
?
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
-
PP2A plays an important role in control of proto-oncogene c-Myc by destabilization of c-Myc and promotion of its ubiquitin-mediated degradation, PP2a has tumor-suppressor activity, c-Myc regulation is essential for normal cell function, overview
-
-
?
phosphorylated Ca2+/calmodulin-dependent protein kinase II + H2O
Ca2+/calmodulin-dependent protein kinase II + phosphate
show the reaction diagram
-
dephosphorylation of the autophosphorylated Thr286, PPM1F negatively regulates the multifunctional Ca2+/calmodulin-dependent protein kinase II, e.g. reducing the phosphorylation of vimentin
-
-
?
phosphorylated Cav1.2 + H2O
Cav1.2 + phosphate
show the reaction diagram
Q28653
binding of PP2A to the L-type calcium channel Cav1.2 next to Ser1928, its main PKA site, is critical for Ser1928 dephosphorylation
-
-
?
phosphorylated Cbp1 + H2o
Cbp1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated cyclin G1 + H2O
cyclin G1 + phosphate
show the reaction diagram
-
the B'alpha1 subunit of the serine/threonine protein phosphatase 2A, which binds to cyclin G1, can stabilize cyclin G1 under unstressed conditions and upon DNA damage, as well as inhibit the ability of cyclin G1 to be ubiquitinated
-
-
?
phosphorylated DNA-dependent protein kinase + H2O
DNA-dependent protein kinase + phosphate
show the reaction diagram
-
activation of DNA-dependent protein kinase to repair DNA double strand breaks by the process of nonhomologous end joining
-
-
?
phosphorylated Ebola virus VP30 protein + H2O
Ebola virus VP30 protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ERK1 + H2O
ERK1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ERK2 + H2O
ERK2 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated ERK2 + H2O
ERK2 + phosphate
show the reaction diagram
-
PP2A acts as a gate-keeper of extracellular signal-regulated kinase, ERK, activity in neuronal PC12 cells, the PP2A regulatory subunits Balpha and Bdelta target the PP2A heterotrimer to dephosphorylate and inactivate ERK, silencing of B subunits leads to hyperactivation of ERK stimulated by constitutively active MEK1, overview
-
-
?
phosphorylated glycogen phosphorylase a + H2O
glycogen phosphorylase a + phosphate
show the reaction diagram
-
inactivation of the substrate enzyme, overview
-
-
?
phosphorylated H2AX + H2O
H2AX + phosphate
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae AY925
-
the Pph3p complex is required for dephosphorylation of H2AX, Pph21p (PP2Ac ortholog) is not involved in H2AX dephosphorylation
-
-
?
phosphorylated High mobility group box 1 protein + H2O
High mobility group box 1 protein + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated histone deacetylase 3 + H2O
histone deacetylase 3 + phosphate
show the reaction diagram
-
dephosphorylation at Ser424, PP4 negatively regulates the activity of histone deacetylase 3 by direct protein-protein interaction also involving the so-repressors N-CoR and SMRT, PP4 regulates histone deacetylase 3, HDAC3, activity by dephosphorylation of Ser424, and by direct protein-protein interaction of active PP4 which is complexed with HDAC3 via the N-terminus of the deacetylase, PP4 is the limiting factor and is not saturating in the complex in vivo
-
-
?
phosphorylated IkappaB kinase + H2O
IkappaB kinase + phosphate
show the reaction diagram
Mus musculus, Mus musculus C57BL6
-
PP2A positively regulates multicomponent Ikappa kinase signaling, eventhough it inhibits its activity by dephosphorylation, positive regulation requires PP2A-IKK complex formation, suppression of the complex formation attenuates IKK T loop phosphorylation and activation, PP2A inhibition attenuates TNFalpha-induced IKK degradation, overview
-
-
?
phosphorylated ILK1 + H2O
ILK1 + phosphate
show the reaction diagram
-
negative regulation of Wnt signaling
-
-
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
-
the substrate enzyme regulates the activity of the Ca2+-ATPase pump on the sarcoplasmic reticulum and is a key regulator of both basal contractility and the heart's beta-agonist responses, overview
dephosphorylated phospholamban exhibits an inhibitory effect on SERCA2a
-
?
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
dephosphorylation at Thr696
-
-
?
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
-
isozyme PP1delta regulates smooth muscle relaxation
-
-
?
phosphorylated nucleoside-diphosphate kinase + H2O
nucleoside-diphosphate kinase + phosphate
show the reaction diagram
-
dephosphorylation of nucleoside-diphosphate kinase leads to loss of enzymatic activity, an efficient mode of posttranslational modification of the enzyme crucial to cellular development. Decreasing order of efficiency: nucleoside-diphosphate kinase from Homo sapiens, nucleoside-diphosphate kinase from Pseudomonas aeruginosa, nucleoside-diphosphate kinase from Escherichia coli, nucleoside-diphosphate kinase from yeast
-
-
?
phosphorylated p38 + H2O
p38 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated p38alpha + H2O
p38alpha + phosphate
show the reaction diagram
-
mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
dephosphorylation of Ser15 by PP1, no activity with PP2A, PP1 inhibition leads to hyperphosphorylation of p53, PP1 has regulatory function
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
the B56-containing PP2A acts partly through p53 suppressing apoptosis
-
-
?
phosphorylated penicillin-binding protein + H2O
penicillin-binding protein + phosphate
show the reaction diagram
Enterococcus faecium, Enterococcus faecium D344S
-
-
-
-
?
phosphorylated phosphoenolpyruvate carboxylase + H2O
phosphoenolpyruvate carboxylase + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
isozyme PP1 preferentially dephosphorylates the beta-subunit of phosphorylase kinase, isozyme PP2 preferentially dephosphorylates the alpha-subunit of phosphorylase kinase
-
-
?
phosphorylated pRb protein + H2O
pRb protein + phosphate
show the reaction diagram
-
ceramide prevents retinoblastoma gene product pRb protein hyperphosphorylation by PP1 activation, nerve growth factor deprivation in sympathetic neurons leads to inhibition of PP1 and hyperphosphorylation of pRb protein
-
-
?
phosphorylated protein kinase Hs11 + H2O
protein kinase Hs11 + phosphate
show the reaction diagram
-
contributes to its delocalization and destruction
-
-
?
phosphorylated Prz1 + H2o
Prz1 + phosphate
show the reaction diagram
-
induction of calcineurin itself and the Pmc1 CXa2+ ATPase
-
-
?
phosphorylated Raf-1 kinase + H2O
Raf 1 kinase + phosphate
show the reaction diagram
-
phosphatase 2A dephosphorylates Raf-1 Ser259 in response to mitogens
-
-
?
phosphorylated Raf1 + H2O
Raf-1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated rhodopsin + H2O
rhodopsin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated RNA polymerase II + H2O
RNA polymerase II + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated securin + H2O
securin + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated serine/threonine protein kinase + H2O
serine/threonine protein kinase + phosphate
show the reaction diagram
Enterococcus faecium, Enterococcus faecium D344S
-
-
-
-
?
phosphorylated spliceosome-associated protein 155 + H2O
spliceosome-associated protein 155 + phosphate
show the reaction diagram
-
i.e. Sap155, a U2 small nuclear ribonucleoprotein particle component. nuclear inhibitor of PP1, i.e. NIPP1, a major PP1 interactor in the vertebrate nucleus, recruits PP1 to Sap155
-
-
?
phosphorylated Tcn1/Crz1 + H2O
Tcn1/Crz1 + phosphate
show the reaction diagram
-
promotes its translocation into the nucleus and exposure of a transcription activation domain
-
-
?
Rad53p + H2O
?
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae AY925
-
a complex of Pph3p and Psy2p may dephosphorylate Rad53p after it has been activated and phosphorylated in response to methylmethanesulfonate, but a cisplatin-induced activation and phosphorylation of Rad53p may require a complex of Pph3, Psy4p and Psy2p for dephosphorylation
-
-
?
[protein]-L-serine phosphate + H2O
[protein]-L-serine + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
[protein]-L-serine phosphate + H2O
[protein]-L-serine + phosphate
show the reaction diagram
Sulfolobus acidocaldarius MW001
Q4JAD1, Q4JB88
-
-
-
?
[protein]-L-threonine phosphate + H2O
[protein]-L-threonine + phosphate
show the reaction diagram
Q4JAD1, Q4JB88
-
-
-
?
fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase + H2O
fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase + phosphate
show the reaction diagram
-
heterotrimeric phosphatase 2A catalyzing the dephosphorylation of fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
-
-
?
additional information
?
-
-
ceramide-induced cell survival mechanism, overview
-
-
-
additional information
?
-
A2XN40, A3C4N5, Q9LKF8
in vivo expression and response pattern of PP2A gene to environmental stress
-
-
-
additional information
?
-
-
PP1 contains a surface hydrophobic pocket on the catalytic subunit which is important in recognizing cellular regulators via their consensus RVxF motifs, regulation of the enzyme, overview
-
-
-
additional information
?
-
-
PP2A is a multifunctional enzyme, direct interaction with the adenovirus E4orf4 protein via the regulatory subunit initiation virus-induced growth arrest in yeast cells, signal transduction is mediated by Cdc55 interacting with both PP2A catalytic subunit in absence of the Tpd3 subunit and E4orf4, mechanism involving the Tpd3 yeast subunit, overview
-
-
-
additional information
?
-
-
PP2A is involved in central sensitization, PP2A negatively regulates the phosphorylation of N-methyl-D-aspartate, NMDA, receptors in response to capsaicin induction in the spinal cord
-
-
-
additional information
?
-
-
PP2A is involved in the maintenance of E-cadherin-mediated cell-cell adhesion through recruitment of IQGAP1, an effector of small GTPase Rac
-
-
-
additional information
?
-
-
PP2A modulates Akt activity, role of PP2A holoenzyme and subunits in MAPK signaling pathways, overview
-
-
-
additional information
?
-
-
PP2A regulates a wide variety of cellular signal transduction pathways, and performs positive regulation of Raf1-MEK1/2-ERK1/2 signaling at multiple steps of the pathway, overview
-
-
-
additional information
?
-
-
PP4 activates c-Jun N-terminal kinase JNK-1 and is required for JNK-1 stability in prostate cancer cells, PP4 is involved in the MAPK signaling pathway, overview
-
-
-
additional information
?
-
-
PP4 is involved in TNFalpha signalling, PP4 down-regulates IRS-4 and mediates TNFalpha-induced IRS-4 degradation, overview
-
-
-
additional information
?
-
-
PP5 acts as an effector in Rac GTPase signalling via interaction of its N-terminal TPR domain with Rac-GTP, PP5 inhibition results in blockage of KCNH2 channel stimulation by thyroid hormone and by Rac in pituitary cells, overview
-
-
-
additional information
?
-
P36873
PP5 affects many signaling pathways regulating cell growth and cellular stress response
-
-
-
additional information
?
-
-
PP5 is involved in MAPK signal pathways, PP5 suppresses the hypoxia-induced ASK-1/MKK-4/JNK signaling via two mechanisms, 1. by association to ASK-1, 2. by suppressing the phosphorylation of MKK-4, JNK, and c-Jun, overview
-
-
-
additional information
?
-
-
Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5
-
-
-
additional information
?
-
-
regulation and function of the stably associated calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex, PP2A is negatively regulating the kinase, interaction requires the Ca2+/CaM-binding autoinhibitory domain of CaMKIV
-
-
-
additional information
?
-
-
the cystic fibrosis transmembrane conductance regulator, a cAMP-activated chloride channel at the apical surface of epithelia, is regulated by a direct interaction with the protein phosphatase 2A
-
-
-
additional information
?
-
Q96LZ3
the enzyme is critical in many calcium-dependent signaling transduction pathways
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including circadian rhythm and apoptosis, overview
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including circadian rhythm, moluating the circadian transcription factor FRQ, and apoptosis, overview
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including embryonic development, cell proliferation, cell death, and cancer, overview, PP5 is involved in stress response negatively regulating ASK-1 dependent apoptosis and promoting tumor growth
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including embryonic development, cell proliferation, cell death, and cancer, overview, PP5 is involved in stress response negatively regulating ASK-1 dependent apoptosis and promoting tumor growth, PP2A is regulated by the small t antigen of SV40 and polyomavirus, and is involved in targeting of nuclear transcription factors, e.g. HAND1 and HAND2, overview
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of diverse cellular processes and signaling pathways including embryonic development, cell proliferation, cell death, circadian rhythm and cancer, overview, knockout of the PP2A regulator alpha4 causes p53-regulated apoptosis correlated with increased phosphorylation of p53 and JNK, PP2A is involved in regulation of PERIOD degradation and de/phosphorylation important in cricadian rhythm, overview
-
-
-
additional information
?
-
P62207
the enzyme is involved in regulation of many biological processes, e.g. glycogen metabolism, cell-cycle progression, and muscle relaxation, regulatory mechanism involving myosin phosphatase targeting subunit MYPT1 in smooth muscle relaxation, overview
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome and regulation of chromatin activities, it is involved in spliceosomal assembly via interaction with the survival of motor neuron complexes, PP4 regulates several cellular signalling routes, including the NF-kappaB and the target of rapamycin pathways, PP4 plays a role in DNA damage response and decrease histone deacetylase activity, phylogenetic tree and PPP phosphome, overview
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome, overview, PP4 depletion results in disrupted centrosome maturation and a decreased number of chiasmata between homologous chromosomes during meiosis in oocytes
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome, phylogenetic tree and PPP phosphome, overviewe
-
-
-
additional information
?
-
-
the holoenzyme plays a role in organelle assembly and is essential for maturation of the centrosome, PP4 homologues participate in the nutrient sensing pathway, pathway modeling overview, and confers resistance to the anticancer DNA-binding agents cisplatin and oxaliplatin, phylogenetic tree and PPP phosphome, overview
-
-
-
additional information
?
-
-
the Pro33 isoform of integrin beta3 enhances outside-in signaling in platelets by regulating the activation of serine/threonine phosphatases
-
-
-
additional information
?
-
-
PP2A does not modify the activity of no receptor potential A-protein
-
-
-
additional information
?
-
-
PP2Ac associates with the integrin alphaIIbbeta3 complex in platelets, the alphaIIb membrane proximal region containing the KVGFFKR sequence can support the direct interaction of PP2Ac
-
-
-
additional information
?
-
P53041
PP5 binds the A-subunit of the PP2A holoenzyme, which functions to tether the catalytic (PP2Ac) and substrate targeting B-subunits of PP2A, PP5 is a potentially important regulator of both hormone- and stress-induced signaling networks that enable a cell to respond appropriately to genomic stress
-
-
-
additional information
?
-
-
the enzyme is involved in bacterial swarming activity and biofilm formation, PA3346 is the only of seven orphan response regulators inthe organism that is trans-phosphorylated by HptB
-
-
-
additional information
?
-
-
the expression of wild type PP2A catalytic subunit suppresses the MAPK kinase 3 phosphorylation
-
-
-
additional information
?
-
P49597, Q9FIF5
different mutants: mutations in genes involved in the ethylene signal transduction pathway and a mutation at the start of exon 2
-
-
-
additional information
?
-
-
calcineurin is a Ca2+/calmodulin-dependent protein phosphatase
-
-
-
additional information
?
-
-
physical and functional interaction of the phosphatase calcineurin with a group of modulators, the RCAN protein family, e.g. proteins Cabin1 and AKAP79, a valine-rich region within the RCAN carboxyl region is essential for binding to the docking site in calcineurin, overview. Competition by substrates and modulators for a common docking site appears to be an essential mechanism in the regulation of Ca2+-calcineurin signaling
-
-
-
additional information
?
-
-
regulation of cellular PP1 by phosphorylation of the CPI-17 family, C-kinase-activated PP1 inhibitors, overview
-
-
-
additional information
?
-
-
the enzyme is cleaved by caspase-3, PP1 inhibitor Inh3 harbors a putative caspase-3 cleavage site, 46DTVD49, located between the two PP1 interaction sites, which consist of a RVXF motif and an adjacent inhibitory domain, both of which are required for inhibition of PP1
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Calmodulin
-
activation, dependent on Ca2+
Calmodulin
-
requirement, phosphatase 2B, i.e. calcineurin
Calmodulin
-
requirement, phosphatase 2B, i.e. calcineurin
Calmodulin
-
activation, dependent on Ca2+
Calmodulin
-
no stimulation
Calmodulin
O15920
stimulation of phosphatase PfPP2B
Calmodulin
-
-
Calmodulin
-
-
Calmodulin
-
isozyme PP2B is dependent on calmodulin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
required by B subunit of calcineurin, four binding sites
Ca2+
-
required by B subunit of calcineurin, four binding sites
Ca2+
-
phosphatase 2B
Ca2+
-
calcineurin A, strictly dependent on Mn2+ or Mg2+ or Ca2+
Ca2+
-
phosphatase PP7 activated by Ca2+
Ca2+
-
phosphatase WbPP is stimulated but not dependent on
Ca2+
O15920
phosphatase PfPP2A, Ca2+ can replace Mn2+ in activation; phosphatase PfPP2B, activated by Ca2+ plus calmodulin
Ca2+
-
between one and two Ca2+ ions/protein, bound in the EF-hands of the protein
Ca2+
-
activates; activates, synergistic with calmodulin, activation is completely inhibited by trifluoroperazine
Ca2+
Q96LZ3
the regulatory subunit is an EF-hand Ca2+-binding protein
Ca2+
-
activates
Ca2+
-
isozyme PP2B is dependent on Ca2+
Ca2+
-
calcineurin is dependent on Ca2+/calmodulin
Ca2+
-
calcineurin is dependent on Ca2+/calmodulin
Ca2+
-
calcineurin is dependent on Ca2+/calmodulin
Ca2+
-
calcium-dependent protein phosphatase 2B
Ca2+
P48452
dependent on
Ca2+
-
calcineurin is a Ca2+/calmodulin-activated Ser/Thr phosphatase
Ca2+
-
dependent on
Calmodulin
-
activates the enzyme with substratees phospho-L-serine and phospho-L-threonine, but not with phospho-L-tyrosine
Cd2+
-
20 mM, stimulation is about 45% compared to that with Mn2+
Cd2+
Q4JAD1, Q4JB88
the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive
Co2+
-
-
Co2+
-
-
Co2+
-
stimulates
Co2+
-
20 mM, stimulation is about 15% compared to that with Mn2+
Co2+
Q4JAD1, Q4JB88
the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive
Co2+
O08367
activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM
Fe2+
-
phosphatase 2B, i.e. calcineurin, metalloenzyme
Fe2+
-
Mn2+ and Fe2+ stimulate
Fe2+
P36873
trace amounts
Fe2+
-
isozyme PP1 contains one Fe2+ and isozyme PP2B contains one Fe2+ at the centre of the catalytic site
Fe2+
P48452
activates the eye isozyme, but might inhibit the brain isozyme
Mg2+
-
-
Mg2+
-
phosphatase type PP2Calpha not stimulated
Mg2+
-
calcineurin A, strictly dependent on Mn2+ or Mg2+ or Ca2+
Mg2+
-
phosphatase PP7 dependent on Mg2+
Mg2+
-
phosphatase WbPP is stimulated but not dependent on
Mg2+
-
; better activator than Mn2+
Mg2+
-
when p-nitrophenyl phosphate is used as substrate 10 mM MgCl2 increases the activity, when phosphocasein is used as substrate 10 mM MgCl2 reduces the activity by 50%
Mg2+
-
essential for activity
Mg2+
-
metal required for activity. Order of decreasing efficiency: Mg2+, Mn2+, Na+
Mg2+
-
activates
Mg2+
-
activates, 40fold less effective than Mn2+ at pH 9.5
Mg2+
-
dependent
Mg2+
-
dependent on
Mg2+
Q5AME8
dependent on
Mg2+
-
SCPL-1 is dependent on Mg2+
Mg2+
-
dependent on
Mg2+
-
-
Mg2+
-
stimulates
Mg2+
Q4JAD1, Q4JB88
the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive
Mg2+
O08367
activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation with Mg2+ is induced between 5 and 10 mM
Mg2+
-
-
Mn2+
-
activation; depending on source of enzyme and substrate
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
activation
Mn2+
-
calcineurin A, strictly dependent on Mn2+ or Mg2+ or Ca2+
Mn2+
-
active only in presence of Mn2+, optimal concentration 5-6 mM, inactive in presence of other divalent metal ions
Mn2+
-
-
Mn2+
-
Mn2+ and Fe2+ stimulate
Mn2+
O15920
required by phosphatase PfPP2A
Mn2+
-
dependent, replacement of Mn2+ with other divalent cations lowers the activity of the enzyme
Mn2+
-
; better activator than Mg2+
Mn2+
-
metal required for activity. Order of decreasing efficiency: Mg2+, Mn2+, Na+
Mn2+
-
-
Mn2+
-
required
Mn2+
P36873
1 ion per enzyme molecule, involved in metal-mediated substrate hydrolysis
Mn2+
-
activates, 40fold more effective than Mg2+ at pH 9.5
Mn2+
P62207
required for full activiy
Mn2+
-
the enzyme contains a two-metal center
Mn2+
-
activating effect at 2 mM
Mn2+
-
required for activity
Mn2+
-
dependent
Mn2+
-
dependent on
Mn2+
-
isozyme PP1 activity is dependent on Mn2+ with 2.1fold stimulation at 3 mM
Mn2+
-
1 mM, Mn2+ is the most effective cofactor
Mn2+
-
isozyme PP2A contains two Mn2+ and isozyme PP1 contains one Mn2+ at the centre of the catalytic site
Mn2+
P48452
slight stimulation
Mn2+
-
stimulates
Mn2+
-
20 mM, stimulates
Mn2+
Q4JAD1, Q4JB88
the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive
Mn2+
O08367
activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+ is the most effective divalent metal ion. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM
Mn2+
-
the catalytic active site contains two Mn2+ ions, Mn2+-bound enzyme always exhibits the highest activity and stability
Mn2+
-
activates
Mn2+
-
Mn2+ is required for activity. Reduction of the Mn2+ concentration from 2 mM to 0.05 mM leads to a 100fold decrease in the catalytic efficiency of wild type enzyme
Na+
-
metal required for activity. Order of decreasing efficiency: Mg2+, Mn2+, Na+
Ni2+
-
activation
Ni2+
-
activation
Ni2+
O15920
phosphatase PfPP2A, Ni2+ can replace Mn2+ in activation
Ni2+
-
; best activator; best activator for P5
Ni2+
-
PP2B but not PP1 is stimulated by Ni2+
Ni2+
P48452
strong activation
Ni2+
-
stimulates
Ni2+
-
20 mM, stimulation is about 5% compared to that with Mn2+
Ni2+
Q4JAD1, Q4JB88
the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive
Ni2+
O08367
activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM
Zn2+
-
-
Zn2+
-
phosphatase 2B, i.e. calcineurin, metalloenzyme
Zn2+
-
-
Zn2+
-
-
Zn2+
P36873
trace amounts
Zn2+
-
isozyme PP2B contains one Zn2+ at the centre of the catalytic site
Mn2+
-
activation
additional information
-
phosphatase PP3 active in the absence of divalent cations
additional information
-
heterotrimeric phosphatase 2A catalyzing the dephosphorylation of fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase is not dependent on Mg2+, Ca2+, or Mn2+
additional information
-
no effect by Ca2+ and Zn2+, metal ion preference depends strongly on the pH value, overview
additional information
P62207
one of the two metal ions in the metal center of the enzyme might as well be Fe2+ instead of Mn2+
additional information
-
addition of Mg2+ and Ca2+ does not affect the enzyme activity
additional information
-
Ca2+, Cu2+, or Zn2+ have little or no stimulatory effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,3-bis(4-amino-2-methylquinolin-6-yl)urea
P35813
-
1-(3-bromophenyl)-6-undecyl-1,3,5-triazinane-2,4-diimine
P35813
-
1-[(ethylcarbamoyl)amino]-1-oxopropan-2-yl (3E)-3-(3,4-dimethoxybenzylidene)-2,3-dihydro-1H-cyclopenta[b]quinoline-9-carboxylate
-
-
-
2-amino-4-methyl-N-naphthalen-2-ylpentanamide
P35813
-
2-mercaptoethanol
-
phosphatase activity is decreased by 5 mM
3-hydroxy-3-methylglutaryl CoA
-
-
3-hydroxy-3-methylglutaryl CoA
-
-
3-iodo-7-[(8-iodo-10-phenyl-5,10-dihydrophenazin-2-yl)amino]-5-phenylphenazin-5-ium
P35813
-
4,4'-dipyridyl disulfide
-
-
4-nitrophenyl phosphate
-
10 mM, 89% inhibition
5,5',6,6',8,8'-hexahydroxy-2,2',3,3'-tetramethyl-2,2',3,3'-tetrahydro-4H,4'H-9,9'-bibenzo[g]chromene-4,4'-dione
P35813
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
6,6-dimethyl-1-[4-(4-phenylbutyl)phenyl]-1,3,5-triazinane-2,4-diimine
P35813
-
6-(3,4-dichloro-phenyl)-4-(N,N-dimethylaminoethylthio)-2-phenyl-pyrimidine
-
i.e. CN585, noncompetitively and reversibly inhibits calcineurin activity, without affecting other Ser/Thr protein phosphatases or peptidyl prolyl cis/trans isomerases. The compound shows potent immunosuppressive effects by inhibiting NFAT nuclear translocation and transactivation, cytokine production, and T cell proliferation
A23187
-
reduces the Ca2+ level and thereby inhibits Ca2+-dependent calcineurin
acetyl-CoA
-
-
ADP
-
1.4 mM, 30C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 12% with phosphorylase a
alpha 4 protein
-
rapamycin has no effect on PP2A-C association with the inhibitor alpha 4 protein
-
AMP
-
1.4 mM, 30C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 10% with phosphorylase a
ATP
-
phosphatases SMP-I and SMP-II
ATP
-
1.4 mM, 30C, pH 7.5, 47% remaining activity with glycogen synthase D as substrate and 15% with phosphorylase a
Bovine serum albumin
-
1 mg/ml 30C, pH 7.5, 95% remaining activity with glycogen synthase D and 92% with phosphorylase, in presence of 10 mg/ml 79% remaining activity with phosphorylase a as substrate
-
c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
-
-
C-terminally truncated nuclear inhibitor of protein phosphatase-1
-
C-terminally truncated NIPP1 forms a hyperactive holoenzyme with PP1, rendering PP1 minimally phosphorylated on an inhibitory site
-
Ca2+
-
high concentrations
Ca2+
-
degree of inhibition depends on concentration of Mg2+
CaCl2
-
14 mM, 30C, pH 7.5, with phosphorylase a as substrate, 50% remaining activity
caffeic acid phenethyl ester
-
down-regulation of expression of PP2A catalytic subunit in cells treated with caffeic acid phenethyl ester at 48 h
calyculin
-
IC50: 0.09 nM
calyculin
-
moderate inhibition at 0.00001 mM
calyculin A
O15757
-
calyculin A
-
inhibits isozymes PP1, PP4, PP5, and PP2A
calyculin A
-
inhibits PP1, PP4, PP5, and PP2A
calyculin A
-
-
calyculin A
-
inhibitor of isozymes PP1 and PP2A
calyculin A
P48452
-
calyculin-A
P67775
-
cAMP
-
1 mM, 30C, pH 7.5, 83% remaining activity with histone as substrate and 83% with phosphorylase a
cantharidin
-
-
cantharidin
-
IC50: 60 nM
cantharidin
-
IC50: 0.13 mM
cantharidin
-
antitumor agent from the chinese blister beetle, strong inhibition of PP4
cantharidin
-
-
cantharidin
P48452
-
cantharidin
-
-
cantharidin
-
-
cantharidine
P67775
-
-
cantheridin
-
strong inhibition at 0.005 mM
caspase-3-inhibitor
-
0.1 mM completely inhibits activity
citrate
-
1.4 mM, 30C, pH 7.5, 68% remaining activity with glycogen synthase D as substrate
Co2+
-
Mn2+-stimulated form
Co2+
-
-
Co2+
-
1 mM: 8% of enzyme activity
CPI-17
-
encoded by gene PPP1R14A, chromosome 19. Upon phosphorylation at Thr38, the 17 kDa PP1 inhibitor protein, CPI-17, selectively inhibits a specific form of PP1, myosin light chain phosphatase, which transduces multiple kinase signals into the phosphorylation of myosin II and other proteins. Phosphorylation of CPI-17 at Thr38 is necessary and sufficient to convert the protein into a potent MLCP inhibitor, mechanism, modeling, overview
-
creatine phosphate
-
60 mM
Cu2+
O08367
-
cyclic phosphopeptide c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
-
inhibits only Wip1
cyclic phosphopeptide c(MpSIpY)
-
-
cyclic phosphopeptide c(MpSIpYV)
-
-
cyclic phosphopeptide c(MpSIpYVA)
-
inhibits only Wip1
cyclic phosphopeptide c(MpSIpYVAT)
-
-
cyclic phosphopeptide c(TDDEMpSIpYVAT)
-
-
cyclin-dependent kinase 2
-
-
-
cyclophilin
-
-
-
cyclosporin
-
PP-2B
cyclosporin
-
-
cyclosporin
Q28653
-
cyclosporin
-
a calcineurin inhibitor, CNI, with potent impact on the success of organ transplantation. The compound is nephrotoxic and can cause renal dysfunction, which is an independent risk factor for graft loss and mortality after kidney transplantation, mechanisms and involved factors, detailed overview
cyclosporin A
O15920
inhibition of phosphatase PfPP2B
cyclosporin A
-
specific inhibitor of calcineurin
cyclosporin A
-
a specific calcineurin inhibitor, time course of inhibition in vivo, overview
cyclosporin A
-
in vivo inhibition in cell culture, 40% inhibition at 0.002 mM in vitro
cyclosporin A
-
a specific pharmacological inhibitor of calcineurin. Cyclosporin A modulates cell shape and actin organization of melanoma cells
cyclosporin A
-
the calcineurin inhibitor can block T-cell activation and is used as immunosuppressiv drug to prevent graft-versus-host reactions and autoimmune diseases
cyclosporin A
-
an immunosuppressive drug. 25% inhibition in keratinocytes at 831 nM, 49% in melanocytes
cyclosporin A
P48452
-
cyclosporin A
-
-
cyclosporin A-cyclophilin complex
-
-
-
cypermethrin
Q28653
-
D,L-Polylysine
-
-
D-glucose 6-phosphate
-
-
DARPP-32
-
-
-
deltamethrin
-
a calcineurin inhibitor
dephospho-CoA
-
-
di-mu-chloro(bis[1-[(morpholin-4-yl-kappaN)methyl]naphthalen-2-olato-kappaO])dicopper
P35813
-
di-mu-chloro(bis[1-[(piperidin-1-yl-kappaN)methyl]naphthalen-2-olato kappaO])dicopper
P35813
-
diethyldicarbonate
-
-
diethyldicarbonate
O08367
0.5%. No inhibition at 0.05% and 0.1%
Diethylpyrocarbonate
-
-
dinophysistoxin-2
P67775
-
diphosphate
-
-
diphosphate
-
-
diphosphate
-
-
diphosphate
-
IC50: 0.07 mM
diphosphate
-
-
diphosphate
-
10 mM, 93% inhibition
diphosphate
-
10 mM, complete inhibition
dithiothreitol
-
phosphatase activity is decreased by 5 mM
docetaxel
-
docetaxel induces the decrease in the activity of protein phosphatase 1
Drosophila melanogaster protein phosphatase inhibitor-2
-
IC50: 0.5-1 nM
-
DSCR1
-
a calcineurin inhibitor, encoded by gene Dscr1 on chromosome 21, is elevated in Down syndrome individuals. Increased dosage of Dyrk1A, appears to act in concert with Dscr1 to suppress tumor angiogenesis by further attenuating VEGF-calcineurin-NFAT signalling in endothelial cells, mechanism, overview. DSCR1-dependent inhibition of the VEGF-calcineurin-NFAT pathway in endothelial cells as a key component of the reduced cancer incidence in Down syndrome individuals
-
DSCR1
-
a calcineurin inhibitor, encoded by gene Dscr1 on chromosome 21, is elevated in Down syndrome model mice
-
EDTA
-
above 5 mM
EDTA
-
type PP2C phosphatase
EDTA
-
14 mM, 30C, pH 7.5, 23% remaining activity with glycogen synthase D as substrate and 35% with phosphorylase a
EDTA
-
0.1 mM, complete inactivation
EDTA
-
0.1 mM decreases specific and total activities by about 40%
EDTA
-
isozyme PP1 has low activity after depletion of Mn2+ with EDTA
EDTA
-
10 mM, 93% inhibition
EDTA
O08367
-
EGTA
-
14 mM, 30C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 83% with phosphorylase a
EGTA
-
moderate inhibition
EGTA
-
reduces the Ca2+ level and thereby inhibits Ca2+-dependent calcineurin
EGTA
-
-
endophilin B1t
-
-
-
endothall
-
-
endothall
-
IC50: 0.21 mM
endothall
-
specific inhibitor of isozyme PP2A
endothall
-
-
F-
-
phosphatase SMP-II
Fe2+
-
Mn2+-stimulated form
Fe2+
-
1 mM: 7% of enzyme activity
Fe2+
O08367
-
Fe3+
-
1 mM: 2% of enzyme activity
FK506
-
selective PPP3 inhibitor
FK506
-
a specific calcineurin inhibitor, time course of inhibition in vivo, overview
FK506
-
a calcineurin inhibitor
FK506
-
the calcineurin inhibitor can block T-cell activation and is used as immunosuppressive drug to prevent graft-versus-host reactions and autoimmune diseases
FK506
P48452
-
FK506
P36873
-
FK506-FK506-binding protein
-
-
-
fostriecin
-
IC50: 0.022 mM
fostriecin
Q28653
-
fostriecin
-
potent specific inhibition of PP2A
fostriecin
-
inhibits isozymes PP2A and PP4
fostriecin
-
inhibits PP2A and PP4
fostriecin
-
antitumor agent from Streptomyces pulveraceus ssp. fostreus, strong inhibition of PP4
fostriecin
-
inhibits PP2A at 3.2 nM
fostriecin
P61292
inhibits at 400-1000 nM
fostriecin
P48452
-
fostriecin
-
-
fostriecin
-
more selective inhibitor of PP2A
FR225659
-
synthesizes by Helicomyces sp. strain 19353, inhibits PP1 and PP2A, inhibition of gluconeogenese in vivo in hepatocytes
FR253761
-
synthetic derivative of FR225659, inhibits PP1 and PP2A, inhibition of gluconeogenese in vivo in hepatocytes
FR259383
-
synthetic derivative of FR225659, inhibits PP1 and PP2A, inhibition of gluconeogenese in vivo in hepatocytes
Gelatin
-
1 mg/ml, 30C, pH 7.5, 92% remaining activity with histone as substrate, and 31% withe phosphorylase a, in presence of 10 mg/ml 10% remaining activity with phohsphorylase a
-
ginsenoside Rg1
P63329
from Panax ginseng roots, Rg1 alleviates left ventricular hypertrophy induced by abdominal aorta coarctation, and the protection appears to be due, at least in part, to its inhibitory effects on calcineurin and MAP kinase signaling pathways, overview
glutamate
-
inhibitor of isozyme PP2A
glutathione disulfide
-
-
guanosine 5'-(beta,gamma-imido)triphosphate
-
-
heparin
-
-
histone
-
competitive when glycogen synthase D is the substrate, increases the Km-value for glycogen synthase D 4fold, but shows mixed type inhibition when phosphorylase a acts as substrate with an 1.4fold increase of Km
histone H1
-
-
-
human Inhibitor-3
-
possesses a putative protein phosphatase-1 binding motif, 39KKVEW43. A second interaction site, whose deletion leads to loss of inhibitory potency, is identified between residues 65-77
-
I-1 protein
-
-
-
I-2 protein
-
PP1-specific inhibitor
-
Inhibitor protein
-
MW 33000-36000, heat and acid stable, specific for BCKDH phosphatase
-
Inhibitor-1
-
inhibitor of isozyme PP1
-
Inhibitor-2
-
marginally inhibited by 200 nM
-
Inhibitor-2
-
-
-
Inhibitor-2
P61292
inhibits at 100-500 nM
-
Inhibitor-2
-
inhibitor of isozyme PP1
-
Inhibitor-2
-
-
-
Inhibitor-2
-
-
-
isobutyryl-CoA
-
-
isovaleryl-CoA
-
-
L-Malate
-
-
mesalazine
-
1 mg/ml efficiently inhibits PP2A
MgCl2
-
14 mM, 30C, pH 7.5, with phosphorylase a as substrate, 60% remaining activity
micocystin
-
potent inhibitor
-
microcystin
O15757
-
-
microcystin
-
PP-1
-
microcystin
-
-
-
microcystin
Q28653
-
-
microcystin
-
inhibits isozymes PP1, PP4, PP5, and PP2A
-
microcystin
-
inhibits PP1, PP4, PP5, and PP2A
-
microcystin
-
inhibits by binding tightly to the phosphatase catalytic domain of PP5
-
microcystin-LR
-
inhibition of phosphatase PP3
microcystin-LR
-
-
microcystin-LR
-
-
microcystin-LR
-
IC50: 0.01 nM
microcystin-LR
-
-
microcystin-LR
-
specific PP2A inhibitor
microcystin-LR
-
inhibitor of isozymes PP1 and PP2A
microcystin-LR
P48452
-
microcystin-LR
P36873
-
microcystin-RR
-
-
microsystin-LR
P67775
-
-
MnCl2
-
14 mM, 30C, pH 7.5, with phosphorylase a as substrate, 51% remaining activity
myosin light chain hydroxy peptide
-
competitive inhibition
-
N-ethylmaleimide
-
-
N-methyl-N'-phenanthren-9-ylimidodicarbonimidic diamide
P35813
-
Na2HPO4
-
14 mM, 30C, pH 7.5, no activity with glycogen synthase D as substrate, remaining activity 79% with histone as substrate, and 64% with phosphorylase a as substrate
Na2P2O7
-
14 mM, 30C, pH 7.5, remaining activity 6% with glycogen synthase D as substrate, 15% with histone as substrate, and 8% with phosphorylase a as substrate
-
Na2SO4
-
14 mM, 30C, pH 7.5, remaining activity 53% with glycogen synthase D as substrate and 32% with phosphorylase a as substrate
Na4P2O7
-
-
NaCl
-
14 mM, 30C, pH 7.5, with phosphorylase a as substrate, 83% remaining activity
NaF
-
type PP2C phosphatase
NaF
-
IC50: 2.1 mM
NaF
-
14 mM, 30C, pH 7.5, remaining activity 31% with glycogen synthase D as substrate, 67% with histone as substrate, and 37% with phosphorylase a as substrate
NaF
-
specific inhibition of activity for the RRApTVA substrate by 50 mM
NaF
-
moderate inhibition; strong inhibition
NaF
-
50 mM, 78% inhibition
NaF
-
50 mM, 47% inhibition
NaF
O08367
-
NaH2PO4
-
-
NaHCO3
-
14 mM, 30C, pH 7.5, remaining activity 49% with glycogen synthase D as substrate, 79% with histone as substrate, and 64% with phosphorylase a as substrate
NaNO3
-
14 mM, 30C, pH 7.5, remaining activity 70% with glycogen synthase D as substrate, 83% with histone as substrate, and 78% with phosphorylase a as substrate
neurabin I
-
-
-
neurabin-I
-
IC50 for the wild-type catalytic subunit PP1alpha is 1.75 nM
-
Ni2+
-
-
Ni2+
-
1 mM: 4% of enzyme activity
nodularin
-
IC50: 0.04 nM
nodularin
-
IC50: 0.0005 mM
nodularin
-
inhibits isozymes PP2A, PP5, and PP4
nodularin
-
inhibits PP2A, PP5, and PP4
nuclear inhibitor of protein phosphatase 1
-
-
-
Nucleoside mono-, di-, and triphosphates
-
e.g. GTP, GDP, ATP, ADP
-
Nucleoside mono-, di-, and triphosphates
-
e.g. GTP, GDP, ATP, ADP
-
Nucleoside mono-, di-, and triphosphates
-
e.g. GTP, GDP, ATP, ADP
-
Nucleoside mono-, di-, and triphosphates
-
e.g. GTP, GDP, ATP, ADP
-
ocadaic acid
-
-
okadaic acid
-
inhibition of phosphatase PP3
okadaic acid
-
inhibition of heterotrimeric phosphatase 2A catalyzing the dephosphorylation of fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
okadaic acid
-
-
okadaic acid
-
two enzyme forms in parotid gland, one is sensitive, the other not inhibited
okadaic acid
-
no inhibition of phosphatase PP2Calpha
okadaic acid
-
type PP2C phosphatase is not inhibited
okadaic acid
-
no inhibition of phosphatase PP2C
okadaic acid
-
no inhibition of phosphatase PP7
okadaic acid
-
0.00125 mM. 50% inhibition
okadaic acid
O15920
no inhibition
okadaic acid
-
IC50: 40 nM
okadaic acid
O15757
-
okadaic acid
-
IC50: 0.18 nM; IC50: 0.1 nM
okadaic acid
-
IC50: 20 nM
okadaic acid
-
IC50: 0.66 nM
okadaic acid
-
IC50: 0.0002 mM
okadaic acid
-
-
okadaic acid
-
PP-2A
okadaic acid
-
IC50: 4 nM
okadaic acid
Q84K11
IC50: 0.0081 mM
okadaic acid
-
IC50: 0.03 nM
okadaic acid
-
inhibits isozymes PP1, PP4, PP5, and PP2A
okadaic acid
-
inhibits PP1, PP4, PP5, and PP2A
okadaic acid
-
IC50 for the wild-type catalytic subunit PP1alpha is 157 nM
okadaic acid
-
specific
okadaic acid
-
PP2A inhibition induces the dissociation of IQGAP1 from the E-cadheri-catenins complex
okadaic acid
-
inhibition of wild-type PP5, no inhibition of PP5 mutant 451A
okadaic acid
-
unspecific protein phosphatase inhibitor
okadaic acid
-
inhibits PP2A at 100 nM
okadaic acid
-
50-100 nM efficiently inhibits PP2A
okadaic acid
-
inhibits PP2A at 10 nM
okadaic acid
-
inhibition at 1 nM
okadaic acid
-
inhibition at 100 nM
okadaic acid
-
91% inhibition of the total phosphatase activity
okadaic acid
-
inhibitory at 10 nM
okadaic acid
-
inhibitor of isozyme PP2A
okadaic acid
-
PP2A activity is dose-dependently decreased with okadaic acid with 15% inhibition at 0.05 nM, 55% inhibition at 0.5 nM, and 69% inhibition at 5 nM
okadaic acid
-
complete inhibition at 250 nM
okadaic acid
-
complete inhibition at 50 nM
okadaic acid
-
inhibitor of isozymes PP1 and PP2A
okadaic acid
-
a polyether derivative of a C38 fatty acid produced by toxigenic dinoflagellates, Dynophysis and Prorocentrum, strong inhibition by okadaic acid and derivatives
okadaic acid
Q4JAD1, Q4JB88
56% activity in presence of 10 nM okadaic acid
okadaic acid
O08367
inhibition is greater at 40C than at 90C
okadaic acid
P48452
-
okadaic acid
-
specific inhibitor of PP2A
okadaic acid
Q4QE27
-
orthovanadate
-
specific inhibition of activity for the ENDpYINASL substrate by 0.01 mM
p-bromotetramisole
-
PTP-1B
p-chloromercuribenzoate
-
-
paclitaxel
-
paclitaxel induces decrease in activity of protein phosphatase 2 subgroups
pentafluorophenol
-
-
pervanadate
-
-
-
Phenylarsine oxide
-
potent dithiothreitol-reversible inhibition of PP2A
phosphate
-
-
phosphate
-
-
phosphate
-
-
phosphate
-
-
phosphate
-
competitive inhibition to C-subunit and AC-dimer, noncompetitive inhibition to AC-R2
phosphate
-
IC50: 9.5 mM
phosphate
-
10 mM, 76% inhibition
phosphate
-
50 mM, complete inhibition
phosphate
-
-
phosphocholine
-
-
phosphoenolpyruvate
-
-
phosphoenolpyruvate
-
-
phosphonothioic acid
P48452
-
-
phosphonothioic acid
P36873
-
-
phosphopeptide TDDEMpS-cyclohexylalanine-pYVAT
-
40% inhibition at 0.1 mM
phosphopeptide TDDEMpS-D-pYVAT
-
32% inhibition at 0.1 mM
phosphopeptide TDDEMpS-I-pYVAT
-
54% inhibition at 0.1 mM
phosphopeptide TDDEMpS-P-pYVAT
-
26% inhibition at 0.1 mM
phosphopeptide TDDEMpS-V-pYVAT
-
51% inhibition at 0.1 mM
phosphorylase a
-
mixed type inhibitor with glycogen synthase d as substrate, increases Km 2fold with only small decearse in vmax
pimecrolimus
-
a topical calcineurin inhibitor, treatement might bear high risk for skin cancer and lymphoma, overview
pimecrolimus
-
an immunosuppressive drug
polylysine
-
inhibition with substrate myelin basic protein, activation with substrate p-nitrophenyl phosphate
potassium phosphate
-
-
propionyl-CoA
-
-
protamine
-
inhibition of phosphatase PP3
protein phosphatase inhibitor II
-
specific inhibitor of isozyme PP1
-
Protein phosphatase inhibitor-1
-
-
-
Protein phosphatase inhibitor-1
-
inhibits isozyme PP1
-
Protein phosphatase inhibitor-1
-
inhibits PP1
-
Protein phosphatase inhibitor-1
-
i.e. I-1, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 25 nM
-
Protein phosphatase inhibitor-1
-
PPI-1, the effect of activated inhibitor-1 on regulation of protein phosphorylation is critical in the stimulatory effects of beta-agonists in the heart. The mutant G147D inhibitor-1 decreases PLN phosphorylation in response to beta-adrenergic activation. The G147D polymorphism in PPI-1 occurs exclusively in Afro-American populations, quantitation of the expressional levels of wild-type inhibitor-1 and G147D inhibitor-1, also in white subjects. Overexpression of inhibitor-1 or its variant does not alter the levels of the major regulatory proteins related to cardiac calcium homeostasis
-
protein phosphatase inhibitor-2
-
IC50: 0.65 nM
-
protein phosphatase inhibitor-2
-
-
-
protein phosphatase inhibitor-2
-
i.e. I-2, specific for PP1
-
protein phosphatase inhibitor-2
-
inhibits isozyme PP1
-
protein phosphatase inhibitor-2
-
inhibits PP1
-
protein phosphatase inhibitor-2
-
i.e. I-2, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 2 nM
-
protein phosphatase-1 inhibitor-1
-
the inhibitor-1 contains an RVXF motif sequence, which facilitates its interaction with PP1. On stimulation of the beta-adrenergic axis, protein kinase A phosphorylates Thr35 in inhibitor-1, resulting in PP1 inhibition and amplification of the contractile response
-
protein phosphatase-1 inhibitor-1
-
plays a role in cardiac physiology and pathophysiology, overview
-
protein phosphatase-1 inhibitor-3
-
a potent inhibitor of protein phosphatase-1 that selectively associates with PP1gamma1 and PP1alpha, but not the PP1beta isoform. It is a physiological substrate of caspase-3 harboring a putative caspase-3 cleavage site, 46DTVD49, located between the two PP1 interaction sites, which consist of a RVXF motif and an adjacent inhibitory domain, both of which are required for inhibition of PP1. Inh-3 mutant D49A is caspase-3 resistant
-
rabbit liver glycogen
-
1 mg/ml 30C, pH 7.5, 88% remaining activity with glycogen synthase D and 37% remaining activity with 10 mg/ml inhibitor
-
RCAN proteins
-
RCAN inhibition of CN phosphatase activity is mediated by the extreme C-terminal region
-
Rcn1
-
inhibits calcineurin signaling when expressed at high levels
-
regucalcin
-
has regulatory function in heart cytosol; the protein inhibits the enzyme in presence of EGTA or ethylene glycol and has a regulatory function for the enzyme independently of Ca2+
-
sanguinarine
P48452
-
sanguinarine
P36873
-
small t antigen
-
small t antigen of DNA tumor virus SV40 inhibits the phosphatase activity of the PP2A core enzyme
-
small t antigen of SV40 and polyomavirus
-
inhibits isozyme PP2A
-
small T antigen of SV40 virus
-
specifically inhibits PP2A holoenzymes with B55 or B56 regulatory subunits
-
Sodium diphosphate
-
-
Sodium fluoride
-
-
Sodium fluoride
-
50 mM
sodium orthovanadate
-
1 mM completely inhibits activity
Sodium phosphate
-
-
sodium potassium tartrate
O08367
-
-
Sodium vanadate
-
-
spermine
-
-
succinyl-CoA
-
-
tacrolimus
-
a topical calcineurin inhibitor, treatment might bear high risk for skin cancer and lymphoma, overview
tacrolimus
-
an immunosuppressive drug. 89% inhibition in keratinocytes at 124 nM, 74% in fibroblasts, and 75% in melanocytes
tacrolimus
-
27-30% inhibition at 5 ng/ml, females and males
tacrolimus
-
-
tacrolimus
-
a calcineurin inhibitor, CN, with potent impact on the success of organ transplantation. The compound is nephrotoxic and can cause renal dysfunction, which is an independent risk factor for graft loss and mortality after kidney transplantation, mechanisms and involved factors, detailed overview
Tartrate
-
moderate inhibition of P11 at 2 mM; strong inhibition
tautomycib
P67775
-
-
tautomycin
O15757
-
tautomycin
-
IC50: 0.06 nM
tautomycin
-
inhibits PP1 at 1 nM and PP2A at 10 nM
tautomycin
-
tautomycin preferentially binds PP1 over PP2A
tautomycin
-
inhibitor of isozymes PP1 and PP2A
tautomycin
P48452
-
tetramisole
-
strong inhibition at 2 mM
trifluoperazin
-
cocmplete inhibition at 0.001-0.002 mM
Trifluoperazine
O15920
inhibition of phosphatase PfPP2B
Trifluorperazine
-
moderate inhibition; strong inhibition; strong inhibition at 0.1 mM
tris(2-carboxyethyl)-phosphine hydrochloride
-
phosphatase activity is decreased by 5 mM
Tris-maleate buffer
-
-
tungstate
-
-
UDP
-
1.4 mM, 30C, pH 7.5, 52% remaining activity with glycogen synthase D as substrate and 20% with phosphorylase a
UMP
-
1.4 mM, 30C, pH 7.5, 58% remaining activity with glycogen synthase D as substrate and 16% with phosphorylase a
Urea
-
-
UTP
-
1.4 mM, 30C, pH 7.5, 44% remaining activity with glycogen synthase D as substrate and 21% with phosphorylase a
vanadate
-
at 1 mM inhibition to 60% of its activity
vanadate
-
1 mM, complete inhibition
Zn2+
-
Mn2+-stimulated form
Zn2+
-
-
Zn2+
-
-
Zn2+
-
1 mM: 0% of enzyme activity
Zn2+
P48452
-
Zn2+
-
5 mM, complete inhibition
Zn2+
O08367
-
ZnSO4
-
1 mM, 91% inhibition
molybdate
-
; 1 mM, 70% inhibition
additional information
-
not inhibitory are okadaic acid, phosphoserine, phosphothreonine, phoshotyrosine
-
additional information
-
insensitive to inhibitor-2
-
additional information
-
study of effects of D-glucose 6-phosphate on inhibitory compounds; study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate
-
additional information
-
no inhibition by okadaic acid
-
additional information
-
no inhibition by okadaic acid
-
additional information
-
isozyme PP5 contains an extended N-terminal domain with three tetratricopeptide repeat, TPR, motifs which possesses autoinhibitory function and are important for interaction with other proteins
-
additional information
-
Ppp5 is inhibited by an autoinhitory conformation formed by the N-terminal TPR domain and a C-terminal subdomain, structure and mechanism overview
-
additional information
-
DNA damage 34, i.e. gADD34, and growth arrest disturb the binding of PP1 to p53
-
additional information
-
IC50 of inhibitors for mutant PP1alpha catalytic subunits, overview
-
additional information
-
no inhibition by okadaic acid
-
additional information
-
phosphoserine-containing diphosphorylated peptides with the sequence pSXpY inhibit Wip1 phosphatase activity; PP2A and PP2Calpha are not significantly inhibited by the cyclic phosphopeptides c(MpSIpYVA) and c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
-
additional information
Q8N3J5
the recombinant Trx-His-tagged enzyme is not affected by 0.001 mM okadaic acid
-
additional information
-
not inhibited by tautomycin or cyclosporin
-
additional information
-
20 and 40 nM PPP3CA siRNA decreases PPP3CA protein levels by about 97% but not PPP2CA potein expression, knockdown of PPP3CA protein expression enhances vascular endothelial growth factor-beta, but not fibroblast growth factor 2-stimulated cell proliferation, knockdown of PPP3CA protein expression does not significantly affect vascular endothelial growth factor-induced mitogen-activated protein kinase 3/1 and phosphoinositide 3-kinase/v-akt murine thymoma viral oncogen homolog 1 phosphorylation, while attenuates fibroblast growth factor 2-induced mitogen-activated protein kinase 3/1 and phosphoinositide 3-kinase/v-akt murine thymoma viral oncogen homolog 1 phosphorylation
-
additional information
-
protein phosphatase inhibitor II and cyclosporin A have no inhibitory effect on isozyme PP2A
-
additional information
-
protein phosphatase 2B is highly resistant to inhibition by okadaic acid
-
additional information
-
fibrinogen binding to alphaIIbbeta3 during platelet adhesion decreases integrin-associated PP2A activity
-
additional information
-
PP5 protein expression is more than 90% inhibited by PP5 siRNA transfection
-
additional information
-
SCPL-1 is not inhibited by the typical phosphatase inhibitors NaF, BeCl2, AlCl3, and Na3VO4
-
additional information
-
addition of EGTA and vanadate to the assay does not affect the enzyme activity
-
additional information
-
Par-3 does not inhibit the enzymatic activity of PP1alpha
-
additional information
-
isozyme PP2 is insensitive to inhibitor-1 and inhibitor-2
-
additional information
P49597, Q9FIF5
about 2.4fold reduced expression in Arabidopsis thaliana mutants etr1 with altered gene expression of the ethylene signal transduction pathway; about 3fold reduced expression in Arabidopsis thaliana mutant aba2 with a mutation at the start of exon 2
-
additional information
-
the hypothetic mechanisms of carcinogenesis include a direct effect of calcineurin inhibitors on keratinocytes, because topical calcineurin inhibits DNA repair and reduces apoptosis in epidermal keratinocytes
-
additional information
-
phosphorylation by cyclin-dependent kinases lower PP1 activity in vitro and vivo
-
additional information
-
regulation of cellular PP1 by phosphorylation of the CPI-17 family, C-kinase-activated PP1 inhibitors. Mechanisms underlying PP1 inhibition and the kinase/PP1 cross-talk mediated by CPI-17 and its related proteins, PHI, KEPI, and GBPI, overview
-
additional information
-
knockout of Ste20-like kinase, Mst2, reduces the expression of the catalytic subunit of PP2A, RNAi silencing of Mst2 triggers a striking proteasome-dependent decrease in the levels of the catalytic subunit of PP2A. Treatment with the specific proteasome inhibitor lactacystin completely reverses the loss of HEI-193 cell PP2A-C incurred upon Mst2 RNAi. Mst2-dependent stabilization of PP2A-C is a posttranslational process likely involving a reduction in proteasome-dependent PP2A-C degradation
-
additional information
-
knockout of Ste20-like kinase, Mst2, reduces the expression of the catalytic subunit of PP2A, RNAi silencing of Mst2 triggers a striking proteasome-dependent decrease in the levels of the catalytic subunit of PP2A. Mst2-dependent stabilization of PP2A-C is a posttranslational process likely involving a reduction in proteasome-dependent PP2A-C degradation
-
additional information
-
calcineurin inhibition in fibroblasts and keratinocytes may be of influence on the overall functioning of the skin immune system. No inhibition of calcineurin by okadaic acid
-
additional information
P48452
the residues at 407-456 also have an inhibitory effect on CaN A phosphatase activity in addition to the previously known autoinhibitory domain at 457-480
-
additional information
-
calcineurin inhibitor treatment optimization in patients treated with mixed immunosuppressive protocols, overview
-
additional information
-
activity is unaffected by other potential inhibitors or activators such as polyamines, heparin, cyclic nucleotides, Ca2+/calmodulin, tartrate, tetramisole, okadaic acid, microcystin LR, or sulfhydryl-modifying agents
-
additional information
-
not inhibited by NIPP-1 and inhibitor 2
-
additional information
-
not inhibited by NIPP-1
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(5Z,8Z,14Z)-11,12-epoxyeicosatrienoic acid
-
increases activity 3fold at 0.001 mM
2-mercaptoethanol
-
activation
2-mercaptoethanol
-
phosphatase activity is increased to up to 49-62% by 3 mM
abscisic acid
P49597, Q9FIF5
response to abscisic acid stimulus
adenylyl cyclase AC8
-
from Rattus norvegicus
-
ADP
-
1.4 mM, 30C, pH 7.5, 104% relative activity with histone as substrate
AMP
-
1.4 mM, 30C, pH 7.5, 102% relative activity with histone as substrate
arachidonic acid
-
stimulation of dephosphorylation of phosphorylated myelin basic protein but not phosphorylated casein
arachidonic acid
-
-
arachidonic acid
-
-
arachidonic acid
Q84K11
-
arachidonic acid
-
causes loss of heat shock protein interaction and increases Ppp5 activity, the effect of arachidonic acid on Ppp5 is increased by Rho kinase inhibitor Y27632, which is not affecting alone
arachidonic acid
-
activation mechanism by prompting an alternate conformation of the TPR domain, destabilising the TPR domain-enzyme interface
arachidonic acid
Q4QE27
-
arachidoyl coenzyme A
-
stimulation of dephosphorylation of phosphorylated myelin basic protein but not phosphorylated casein
ATP
-
1.4 mM, 30C, pH 7.5, 107% relative activity with histone as substrate
Bovine serum albumin
-
10 mg/ml, 30C, pH 7.5, 112% relative activity with histone as substrate
-
C2-ceramide
-
potent PP2A activator
C6-ceramide
-
activates in vivo and 2-3fold in vitro, blocked by protein phosphatase inhibitor-2, i.e. I-2, ceramide prevents retinoblastoma gene product pRb hyperphosphorylation by PP1 activation
CaCl2
-
14 mM, 30C, pH 7.5, relative activity 126% with glycogen synthase D as substrate and 232% with histone as substrate compared to no addition
Calmodulin
-
-
Calmodulin
-
-
Calmodulin
-
calcineurin is dependent on Ca2+/calmodulin
Calmodulin
-
calcineurin is dependent on Ca2+/calmodulin
Calmodulin
-
calcineurin is dependent on Ca2+/calmodulin
Calmodulin
P48452
dependent on
Calmodulin
-
calcineurin is a Ca2+/calmodulin-activated Ser/Thr phosphatase
cAMP
-
1 mM, 30C, pH 7.5, 115% relative activity with glycogen synthase D as substrate
ceramide
-
ceramide induces increase of PP2A activity
chlorogenic acid
-
PP2B but not PP1 is stimulated 4fold by 0.1 mM chlorogenic acid
D-Glucosamine 6-phosphate
-
1.4 mM, 30C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 117% with histone as substrate, and 102% with phosphorylase a as substrate compared to no addition
D-glucose
-
1.4 mM, 30C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 101% with histone as substrate, and 106% with phosphorylase a as substrate compared to no addition
D-glucose 6-phosphate
-
1.4 mM, 30C, pH 7.5, 109% relative activity with phosphorylase a as substrate
Detergents
-
e.g. Triton, SDS, activation
dithiothreitol
-
20 mM, 24% activation
dithiothreitol
-
phosphatase activity is increased to up to 49-62% by 0.3 mM
docetaxel
-
docetaxel induces increase in activity of protein phosphatase 2 subgroups
DP71L protein
P61292
expression of DP71L leads to PP1 activation in vivo
-
EDTA
-
14 mM, 30C, pH 7.5, 175% relative activity with histone as substrate
EGTA
-
14 mM, 30C, pH 7.5, 127% relative activity with histone as substrate
ERK-2
O95248, O95278, P28562, P60484, Q05923, Q16828, Q93096, Q9BV47, Q9Y6W6
association with ERK-2 results in catalytic activation of Pac-1
-
Estrogen
-
estrogen maintains the function of PP1, PP2A, and calcineurin
Estrogen
-
estrogen maintains the function of isozymes PP1, PP2A, and calcineurin
ethanol
-
activation
Gelatin
-
1 mg/ml, 30C, pH 7.5, 128% relative activity with glycogen synthase D as substrate, with 10 mg/ml 110% relative activity
-
heparin
-
depending on substrate
heparin
-
causes dissociation to alpha1, beta1 and hence stimulation
histone
-
-
histone
-
activation, depending on substrate
histone H1
-
increased activity
-
HSP70
-
heat shock protein, activation of Ppp5 by dissociation from Hsp70
-
Hsp90
-
heat shock protein, activation of Ppp5 by dissociation from Hsp90
-
Hsp90
-
heat shock protein, activation mechanism via interruption of the TPR domain interactions
-
Inhibitor-2
-
stimulation of phosphatase PP3
-
linoleic acid
-
stimulation of dephosphorylation of phosphorylated myelin basic protein but not phosphorylated casein
linoleic acid
Q84K11
-
linoleic acid
-
increases PP2A expression rate
linolenic acid
Q84K11
-
lipopolysaccharide
-
lipopolysaccharide stimulation induces the expression of PP4
MgCl2
-
14 mM, 30C, pH 7.5, relative activity 256% with glycogen synthase D as substrate and 238% with histone as substrate compared to no addition
Na2SO4
-
14 mM, 30C, pH 7.5, relative activity 152% with histone as substrate compared to no addition
NaCl
-
14 mM, 30C, pH 7.5, relative activity 102% with glycogen synthase D as substrate and 108% with histone as substrate compared to no addition
NaCl
Q5AME8
the transcription of the PTC8 gene is induced with moderate salt concentration environments (0.75 M or 1.0 M NaCl) in Candida albicans cells, and is repressed under high osmolarity
nocodazole
-
causes loss of heat shock protein interaction and increases Ppp5 activity
nuclear inhibitor of protein phosphatase-1
-
NIPP1 directs PP1 to dephosphorylate the U2 small nuclear ribonucleoprotein particle component, spliceosome-associated protein 155, Sap155
-
oleic acid
-
stimulation of dephosphorylation of phosphorylated myelin basic protein but not phosphorylated casein
oleic acid
-
-
oleic acid
Q84K11
-
paclitaxel
-
paclitaxel induces the increase in the activity of protein phosphatase 1
palmitoleic acid
-
stimulation of dephosphorylation of phosphorylated myelin basic protein but not phosphorylated casein
poly-L-lysine
-
-
Polycations
-
e.g. polylysine, protamine, polyarginine, activation
-
polylysine
-
inhibition with substrate myelin basic protein, activation with substrate p-nitrophenyl phosphate
polylysine
-
increased activity
Ppm1p
-
regulates the assembly of PP2A heterotrimers
-
protamine
-
in the presence of ammoniumsulfate; in the presence of ammonium sulfate
protamine
-
-
PTPase activator
-
PTPase activator acts like a chaperone directly affecting the activity of PP2Ac
-
rabbit liver glycogen
-
1 mg/ml, 30C, pH 7.5, 119% relative activity with histone as substrate
-
Rac-GTP
-
stimualtion of PP5, molecular mechanism
-
Rcn1
-
enhances calcineurin signaling when expressed at low levels
-
sulfhydryl reagents
-
activation
TFIIF
-
a transcription factor that binds to the C-terminal domain
-
tris(2-carboxyethyl)-phosphine hydrochloride
-
phosphatase activity is increased to up to 49-62% by 0.3 mM
UMP
-
1.4 mM, 30C, pH 7.5, 102% relative activity with histone as substrate
UTP
-
1.4 mM, 30C, pH 7.5, 108% relative activity with histone as substrate
Xylulose 5-phosphate
-
activation of a heterotrimeric phosphatase 2A catalyzing the dephosphorylation of fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
MnCl2
-
14 mM, 30C, pH 7.5, relative activity 361% with glycogen synthase D as substrate and 280% with histone as substrate compared to no addition
additional information
-
regulated by phosphorylation, phosphatase 2B
-
additional information
-
study of effects of D-glucose 6-phosphate on activating compounds; study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate
-
additional information
-
activation of Ppp involves translocation from the nucleus to the cytoplasm, activation mechanism, overview
-
additional information
-
nerve growth factor is required for PP activation in vivo
-
additional information
-
prolonged hypoxia increases the enzyme expression via activation of a specific transcription factor, the hypoxia-inducible factor-1
-
additional information
-
butyrate induces expression of protein phosphatase when treated to the cell
-
additional information
-
the activity of PP2A is regulated by the bound B56 subunit
-
additional information
-
PAPP2C and its association with phytochromes is enhanced by red light
-
additional information
P53041
an increase in PP5 protein levels is observed following prolonged hypoxia or treatment with reagents that induce oxidative stress
-
additional information
Q5AME8
the expression of CaPTC8 is positively responsive to high temperature or serum-stimulated filamentous growth
-
additional information
-
association of PP2A with transforming growth factor-beta-activated kinase 1 is enhanced by transforming growth factor-beta 1 stimulation
-
additional information
-
PKA is known to phosphorylate and activate PP2A in brain
-
additional information
-
the enzyme is stimulated by the T-cell receptor
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00276
4-nitrophenyl phosphate
-
-
0.53
4-nitrophenyl phosphate
Q4JAD1, Q4JB88
pH 6.5, 70C
0.66
4-nitrophenyl phosphate
-
wild type enzyme, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
0.69
4-nitrophenyl phosphate
-
wild type enzyme, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
1.2
4-nitrophenyl phosphate
-
mutant enzyme T101R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
1.3
4-nitrophenyl phosphate
-
mutant enzyme S120R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
1.5
4-nitrophenyl phosphate
-
mutant enzyme D136Y, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
1.8
4-nitrophenyl phosphate
-
mutant enzyme D136Y, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
2.5
4-nitrophenyl phosphate
Q4JAD1, Q4JB88
pH 6.5, 70C
2.6
4-nitrophenyl phosphate
-
mutant enzyme S120R, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
5.7
4-nitrophenyl phosphate
-
pH 9.5, recombinant His-tagged enzyme
12.7
4-nitrophenyl phosphate
-
pH 7.5, 37, recombinant SynPPM3
0.0027
80S-ribosome
-
30C, pH 7.2
-
0.025
cAMP dependent protein kinase
-
-
-
0.026
DLDVPIPGRFDRRVS(P)VAAE
-
-
0.00063
DLDVPIPGRFDRRVSVAAE
-
-
0.0138
DLDVPIPGRFDRRVSVAAE
-
-
0.0025
DLDVPIPGRFDRRVY(P)VAAE
-
-
0.023
DLDVPIPGRFDRRVY(P)VAAE
-
-
0.4
DRRVS(P)VAAE
-
-
0.0061
DRVY(P)IHPFHL
-
-
0.0189
ENDpYINASL
-
25C, pH 7
0.036
ERK5
-
pH 7.5, 30C, recombinant His-tagged Wip1 catalytic subunit
-
0.49
ETTYERW(pT)TITQRER
Q4JAD1, Q4JB88
pH 6.5, 70C
-
0.213
FDRRVS(P)VAAE
-
-
0.067
FLRT(P)SCG
-
-
0.71
FLRTS(P)CG
-
-
0.096
FLRTT(P)CG
-
-
4.2
fluorotyrosine phosphate
-
-
-
0.0009
FYDEEVDEMY(P)REAPIDKKGNFNY(P)VEFTR
-
-
0.0059
heavy meromyosin
-
-
-
3.8
Mg2+
-
-
0.0015 - 0.0095
myosin light chain
-
-
0.423
NIDAIRA(pS)LNIMSR
Q4JAD1, Q4JB88
pH 6.5, 70C
-
3
p-nitrophenyl phosphate
-
-
10.1
p-nitrophenyl phosphate
-
-
10.3
p-nitrophenyl phosphate
-
pH 7.8, wild-type Prep 2
11.4
p-nitrophenyl phosphate
-
pH 7.8, wild-type Prep 1
16
p-nitrophenyl phosphate
-
pH 7.8, H92N and H22N
17
p-nitrophenyl phosphate
-
pH 7.8, N75H
18
p-nitrophenyl phosphate
-
pH 7.8, H117N
21
p-nitrophenyl phosphate
-
purified, catalytically active 40 kDa-subunit, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30C
34
p-nitrophenyl phosphate
-
full-length Ppt1, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30C
90
p-nitrophenyl phosphate
-
pH 7.8, H186N
200
p-nitrophenyl phosphate
-
30C, pH 7.4
0.00015
phosphocasein
-
-
-
0.0019
phosphocasein
-
-
-
0.002
phosphocasein
-
-
-
0.0023
phosphohistone
-
-
-
0.058
phosphohistone
-
-
-
0.0723
phosphohistone
-
-
-
0.004
phosphohistone H1
-
-
-
0.004
phosphorylase a
-
presence of Mn2+
0.021
phosphorylase a
-
absence of Mn2+
0.01
phosphorylated myosin light chain peptide
-
30C, AC-R2
0.02
phosphorylated myosin light chain peptide
-
30C, AC dimer
0.11
phosphorylated myosin light chain peptide
-
30C, C-subunit
0.00044
phosphoserine-casein
-
pH 7.5, 37, recombinant SynPPM3
-
0.00045
phosphoserine-myelin basic protein
-
pH 7.5, 37, recombinant SynPPM3
-
0.0063
phosphoserine-myelin basic protein
-
pH 7.5, 37, recombinant SynPPM3
-
0.022
phosphoserine-myelin basic protein
-
pH 7.5, 37, recombinant SynPPP1
-
0.0017
phosphoserine-RCML
-
pH 7.5, 37, recombinant SynPPP1
-
0.13
PIPGRFDRRVS(P)VAAE
-
-
0.152
RAT(P)VA
-
-
0.0536
RRA(pT)VA
Q4JAD1, Q4JB88
pH 6.5, 70C
0.058
RRApTVA
-
30C, pH 7.5, D88n
0.076
RRApTVA
-
25C, pH 7
0.338
RRApTVA
-
30C, pH 7.5, wild-type
0.24
RRAS(P)VA
-
pH 9.5, recombinant His-tagged enzyme
-
0.13
RRAT(P)VA
-
pH 9.5, recombinant His-tagged enzyme
-
0.019
serine/threonine-phosphorylated myelin basic protein
-
pH 8.0, recombinant His-tagged enzyme
-
1.7
Tetrafluorotyrosine phosphate
-
-
6.2
tyrosine phosphate
-
-
0.0026
tyrosine-phosphorylated myelin basic protein
-
pH 8.0, recombinant His-tagged enzyme
-
0.001 - 0.003
microtubulin
-
tau-factor
-
additional information
additional information
-
-
-
additional information
additional information
-
alpha-casein: 7 mg/ml, histone IIA: 2.7 mg/ml
-
additional information
additional information
-
kinetics with glycogen synthase D, phosphorylase a, and histone in presence of no, 10, 35, and 100 mM MgCl2, expressed as concentrations of [32P] phosphate
-
additional information
additional information
-
steady-state kinetics, determination of dephosphorylation site-specific kinetic parameters for substrates p38alpha and UNG2, steady-state kinetics for wild-type and mutant p38alpha proteins, overview
-
additional information
additional information
-
very high Km for RRLIEDAEY(P)AARG above accurate detection range
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0015
4-nitrophenyl phosphate
-
mutant enzyme D136Y, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
0.0038
4-nitrophenyl phosphate
-
mutant enzyme S120R, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
0.014
4-nitrophenyl phosphate
-
mutant enzyme T101R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
0.2
4-nitrophenyl phosphate
-
wild type enzyme, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
0.47
4-nitrophenyl phosphate
-
mutant enzyme S120R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
2.33
4-nitrophenyl phosphate
-
mutant enzyme D136Y, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
7.4
4-nitrophenyl phosphate
-
pH 9.5, recombinant His-tagged enzyme
13.8
4-nitrophenyl phosphate
-
-
20
4-nitrophenyl phosphate
-
wild type enzyme, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
2.4
ERK5
-
pH 7.5, 30C, recombinant His-tagged Wip1 catalytic subunit
-
0.1
p-nitrophenyl phosphate
-
pH 7.8, H186N
0.12
p-nitrophenyl phosphate
-
full-length Ppt1, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30C
0.78
p-nitrophenyl phosphate
-
purified, catalytically active 40 kDa-subunit, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30C
3.1
p-nitrophenyl phosphate
-
pH 7.8, N75H
5
p-nitrophenyl phosphate
-
pH 7.8, H22N
490
p-nitrophenyl phosphate
-
pH 7.8, H92N
500
p-nitrophenyl phosphate
-
pH 7.8, wild-type Prep 2
660
p-nitrophenyl phosphate
-
pH 7.8, H117N
1100
p-nitrophenyl phosphate
-
pH 7.8, wild-type Prep 1
2
RRApTVA
-
30C, pH 7.5, D88N
170
RRApTVA
-
30C, pH 7.5, wild-type
0.9
RRAS(P)VA
-
pH 9.5, recombinant His-tagged enzyme
-
2.5
RRAT(P)VA
-
pH 9.5, recombinant His-tagged enzyme
-
1.1
serine/threonine-phosphorylated myelin basic protein
-
pH 8.0, recombinant His-tagged enzyme
-
0.1
tyrosine-phosphorylated myelin basic protein
-
pH 8.0, recombinant His-tagged enzyme
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.001
4-nitrophenyl phosphate
-
mutant enzyme D136Y, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
0.0015
4-nitrophenyl phosphate
-
mutant enzyme S120R, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
0.0115
4-nitrophenyl phosphate
-
mutant enzyme T101R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
0.28
4-nitrophenyl phosphate
-
wild type enzyme, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
0.37
4-nitrophenyl phosphate
-
mutant enzyme S120R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
1.33
4-nitrophenyl phosphate
-
mutant enzyme D136Y, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
30
4-nitrophenyl phosphate
-
wild type enzyme, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37C
136
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0038
6-(3,4-dichloro-phenyl)-4-(N,N-dimethylaminoethylthio)-2-phenyl-pyrimidine
-
-
0.0074
cyclic phosphopeptide c(MpSIpY)
-
at 30C and pH 7.5
0.0029
cyclic phosphopeptide c(MpSIpYV)
-
at 30C and pH 7.5
0.0009
cyclic phosphopeptide c(MpSIpYVA)
-
at 30C and pH 7.5
0.0252
cyclic phosphopeptide c(MpSIpYVAT)
-
at 30CC and pH 7.5
0.035
cyclic phosphopeptide c(TDDEMpSIpYVAT)
-
at 30C and pH 7.5
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0165
c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
-
at 30C and pH 7.5
0.00000009
calyculin
-
IC50: 0.09 nM
0.00006
cantharidin
-
IC50: 60 nM
0.13
cantharidin
-
IC50: 0.13 mM
0.0294
cyclic phosphopeptide c(MpSIpY)
-
at 30?C and pH 7.5
0.0116
cyclic phosphopeptide c(MpSIpYV)
-
at 30C and pH 7.5
0.0037
cyclic phosphopeptide c(MpSIpYVA)
-
at 30C and pH 7.5
0.0999
cyclic phosphopeptide c(MpSIpYVAT)
-
at 30C and pH 7.5
0.1384
cyclic phosphopeptide c(TDDEMpSIpYVAT)
-
at 30C and pH 7.5
0.000002 - 0.0000027
cyclosporin A
-
in fibroblasts
0.00000041
dinophysistoxin-2
P67775
at pH 8.1 and 30C
0.07
diphosphate
-
IC50: 0.07 mM
0.0000005 - 0.000001
Drosophila melanogaster protein phosphatase inhibitor-2
-
IC50: 0.5-1 nM
-
0.0005
endophilin B1t
-
at 37C, in 50 mM Tris-HCl, pH 7.5, 1 mg/ml bovine serum albumin, 1 mM MnCl2, and 0.1% beta-mercaptoethanol
-
0.00009
endothall
-
in 50 mM Tris-HCl (pH 7.4), temperature not specified in the publication
0.21
endothall
-
IC50: 0.21 mM
0.022
fostriecin
-
IC50: 0.022 mM
0.00000001
microcystin-LR
-
IC50: 0.01 nM
2.1
NaF
-
IC50: 2.1 mM
0.000012
neurabin I
-
at 37C, in 50 mM Tris-HCl, pH 7.5, 1 mg/ml bovine serum albumin, 1 mM MnCl2, and 0.1% beta-mercaptoethanol
-
0.00000175
neurabin-I
-
IC50 for the wild-type catalytic subunit PP1alpha is 1.75 nM
-
0.00000004
nodularin
-
IC50: 0.04 nM
0.0005
nodularin
-
IC50: 0.0005 mM
0.00000003
okadaic acid
-
IC50: 0.03 nM
0.00000009
okadaic acid
-
catalytic 38-kDa subunit, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25C
0.0000001
okadaic acid
-
IC50: 0.1 nM
0.0000001
okadaic acid
-
pH and temperature not specified in the publication
0.00000011
okadaic acid
-
holoenzyme, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25C
0.00000018
okadaic acid
-
IC50: 0.18 nM
0.00000023
okadaic acid
P67775
at pH 8.1 and 30C
0.00000066
okadaic acid
-
IC50: 0.66 nM
0.000004
okadaic acid
-
IC50: 4 nM
0.00002
okadaic acid
-
IC50: 20 nM
0.00003
okadaic acid
-
protein phosphatase 1
0.00004
okadaic acid
-
IC50: 40 nM
0.00012
okadaic acid
-
mutant enzyme CNA(1-42)-PP1(9-330)
0.000157
okadaic acid
-
IC50 for the wild-type catalytic subunit PP1alpha is 157 nM
0.0002
okadaic acid
-
IC50: 0.0002 mM
0.005
okadaic acid
-
protein phosphatase 2B
0.0081
okadaic acid
Q84K11
IC50: 0.0081 mM
9.5
phosphate
-
IC50: 9.5 mM
0.000006 - 0.0000201
pimecrolimus
-
in fibroblasts
0.000025
Protein phosphatase inhibitor-1
-
i.e. I-1, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 25 nM
-
0.00000065
protein phosphatase inhibitor-2
-
IC50: 0.65 nM
-
0.000002
protein phosphatase inhibitor-2
-
i.e. I-2, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 2 nM
-
0.0002
small t antigen
-
using phosphorylase a as a substrate
-
0.0000004 - 0.0000013
tacrolimus
-
in fibroblasts
0.00000006
tautomycin
-
IC50: 0.06 nM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0000008 - 0.0000017
-
T-cells from females and males, not stimulated and T-cell-stimulated
0.0001
-
purified recombinant SynPPM3, substrate phosphotyrosine-casein
0.000245
-
wild-type brain
0.000292
-
cytosol extract
0.0007
-
purified recombinant SynPPM3, substrate phosphotyrosine-RCML
0.0017
-
purified recombinant SynPPM3, substrate phosphoserine-RCML; purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein
0.0029
-
purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein
0.003 - 0.015
-
phosphatases PP2Calpha, PP2Cbeta, substrate casein
0.0034
-
purified recombinant SynPPP1, substrate phosphoserine-casein
0.0038 - 0.0077
-
keratinoyctes
0.0044
-
purified recombinant SynPPP1, substrate phosphotyrosine-casein
0.0053
-
purified recombinant SynPPM3, substrate phosphoserine-casein
0.0055
-
purified recombinant SynPPP1, substrate phosphotyrosine-RCML
0.0085 - 0.0127
-
fibroblasts
0.0121
-
purified recombinant SynPPP1, substrate phosphoserine-RCML
0.0178
-
purified recombinant SynPPM3, substrate phosphoserine-myelin basic protein
0.021
O15920
phosphatase PfPP2B
0.033
Q84K11
at pH 7.5 with p-nitrophenyl phosphate as substrate
0.034
Q84K11
at pH 5 with p-nitrophenyl phosphate as substrate
0.034
-
after 117.39fold purification
0.064
-
after thiophosphorylase alpha-Sepharose purification step, with phosphorylated alpha phosphorylase as substrate and in the presence of 0.03 mg protamine and 16 mM ammonium sulfate
0.064
-
at pH 5 with p-nitrophenyl phosphate as substrate
0.066
-
purified recombinant SynPPM3, substrate 4-nitrophenyl phosphate
0.081
-
at pH 7.5 with p-nitrophenyl phosphate as substrate
0.084
-
purified recombinant SynPPP1, substrate 4-nitrophenyl phosphate
0.12
-
after thiophosphorylase alpha-Sepharose purification step, with phosphorylated alpha phosphorylase as substrate and in the presence of 0.03 mg protamine and 16 mM ammonium sulfate
0.13
O15920
phosphatase PfPP2A
0.245
Q84K11
DELTATPR at pH 7.5 with p-nitrophenyl phosphate as substrate
0.49
-
with p-nitrophenyl phosphate as substrate
0.5
-
with phosphorylase kinase as substrate
0.61
-
cell extract, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25C
0.659
-
subunit CNAa-PP1-loop, crude cell lysate
0.665
-
subunit PP1-CNAa-loop, crude cell lysate
1.12
-
with p-nitrophenyl phosphate as substrate
1.2
-
substrate phosphorylase a
1.3
-
wild-type, phosphorylase alpha as substrate
1.8
-
DELTAN-Pph22p, phosphorylase alpha as substrate
2
-
with phosphorylase kinase as substrate
2.5 - 6.5
-
-
3 - 8
-
with p-nitrophenylphosphate as substrate
3
-
with phosphorylase kinase as substrate
4
-
with phosphorylase kinase as substrate
7.087
-
subunit CNAa-PP1-loop, after 10.8fold purification
8
-
with phosphorylase kinase as substrate
10.77
-
subunit PP1-CNAa-loop, after 16.2fold purification
11
-
with p-nitrophenyl phosphate as substrate
12.08
-
after MonoQ FPLC purification step
14
-
with p-nitrophenyl phosphate as substrate
16
-
with p-nitrophenyl phosphate as substrate
19
-
with p-nitrophenyl phosphate as substrate
40
-
with p-nitrophenyl phosphate as substrate
94
P67775
purified enzyme, at pH 8.1 and 30C
310
-
with