Literature summary for 3.1.3.16 extracted from

Azad, M.A.; Sawa, Y.; Ishikawa, T.; Shibata, H.
Purification and characterization of protein phosphatase 2A from petals of the tulip Tulipa gesnerina (2006), J. Biochem. Mol. Biol., 39, 671-676.

Search for more literature for 3.1.3.16

Inhibitors

Inhibitors	Comment	Organism	Structure
Inhibitor-2	marginally inhibited by 200 nM	Tulipa gesneriana
okadaic acid	inhibition at 1 nM	Tulipa gesneriana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	free catalytic subunit, SDS-PAGE	Tulipa gesneriana
65000	-	1 * 75000 + 1 * 65000 + 1 * 38000, SDS-PAGE	Tulipa gesneriana
75000	-	1 * 75000 + 1 * 65000 + 1 * 38000, SDS-PAGE	Tulipa gesneriana

Organism

Organism	UniProt	Comment	Textmining
Tulipa gesneriana	-	cultivar Ile de France	-

Purification (Commentary)

Purification (Comment)	Organism
phenyl-Sepharose CL 4B column chromatgraphy, DEAE-Toyopearl 650 column chromatography, and Microcystin-Sepharose affinity chromatography	Tulipa gesneriana

Source Tissue

Source Tissue	Comment	Organism	Textmining
petal	-	Tulipa gesneriana	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.61	-	cell extract, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C	Tulipa gesneriana
380	-	after 623fold purification, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C	Tulipa gesneriana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl phosphate + H2O	-	Tulipa gesneriana	p-nitrophenol + phosphate	-	?

Subunits

Subunits	Comment	Organism
heterotrimer	1 * 75000 + 1 * 65000 + 1 * 38000, SDS-PAGE	Tulipa gesneriana

Synonyms

Synonyms	Comment	Organism
PP2A	-	Tulipa gesneriana
protein phosphatase 2A	-	Tulipa gesneriana
Ser/Thr phosphatase	-	Tulipa gesneriana

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00000009	-	catalytic 38-kDa subunit, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C	Tulipa gesneriana	okadaic acid
0.00000011	-	holoenzyme, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C	Tulipa gesneriana	okadaic acid

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Release 2023.1 (January 2023)