Cloned (Comment) | Organism |
---|---|
PP2Calpha and PP2Cbeta | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | degree of inhibition depends on concentration of Mg2+ | Bos taurus | |
okadaic acid | no inhibition of phosphatase PP2C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bos taurus | |
Mn2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PP2C | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
photoreceptor | outer segment of rod photoreceptors | Bos taurus | - |
retina | outer segment of rod photoreceptors | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | 0.015 | phosphatases PP2Calpha, PP2Cbeta, substrate casein | Bos taurus |
Storage Stability | Organism |
---|---|
4°C, or -20°C, or -70°C, half-life 24 h, inactivation is not prevented by addition of glycerol, bovine serum albumin, divalent cations or SH-reagents | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphocasein + H2O | - |
Bos taurus | casein + phosphate | - |
? | |
phosphoproteins + H2O | - |
Bos taurus | proteins + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | phosphatase PP2C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | phosphatase PP2C | Bos taurus |