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Literature summary for 3.1.3.16 extracted from
- The Saccharomyces cerevisiae type 2A protein phosphatase Pph22p is biochemically different from mammalian PP2A (2002), Eur. J. Biochem., 269, 3372-3382.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Pichia pastoris GS115 | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | DELTAN-Pph22p, no interaction with protamine and polylysine in contrast to wild-type enzyme | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cantharidin | IC50: 0.13 mM | Saccharomyces cerevisiae |  |
| Co2+ | 1 mM: 8% of enzyme activity | Saccharomyces cerevisiae | |
| endothall | IC50: 0.21 mM | Saccharomyces cerevisiae | |
| Fe2+ | 1 mM: 7% of enzyme activity | Saccharomyces cerevisiae | |
| Fe3+ | 1 mM: 2% of enzyme activity | Saccharomyces cerevisiae | |
| Ni2+ | 1 mM: 4% of enzyme activity | Saccharomyces cerevisiae | |
| nodularin | IC50: 0.0005 mM | Saccharomyces cerevisiae | |
| okadaic acid | IC50: 0.0002 mM | Saccharomyces cerevisiae | |
| Zn2+ | 1 mM: 0% of enzyme activity | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 52000-53000 Da, wild-type, SDS-PAGE, x * 37000, DELTAN-Pph22p, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
wild-type, phosphorylase alpha as substrate | Saccharomyces cerevisiae |
| 1.8 | - |
DELTAN-Pph22p, phosphorylase alpha as substrate | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphorylated phosphorylase alpha + H2O | - |
Saccharomyces cerevisiae | phosphorylase alpha + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000-53000 Da, wild-type, SDS-PAGE, x * 37000, DELTAN-Pph22p, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pph22p | - |
Saccharomyces cerevisiae |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
IC50: 0.0002 mM | Saccharomyces cerevisiae | okadaic acid | |
| 0.0005 | - |
IC50: 0.0005 mM | Saccharomyces cerevisiae | nodularin | |
| 0.13 | - |
IC50: 0.13 mM | Saccharomyces cerevisiae | cantharidin | |
| 0.21 | - |
IC50: 0.21 mM | Saccharomyces cerevisiae | endothall | |
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Release 2023.1 (January 2023)
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