Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arachidonic acid | activation mechanism by prompting an alternate conformation of the TPR domain, destabilising the TPR domain-enzyme interface | Homo sapiens | |
Hsp90 | heat shock protein, activation mechanism via interruption of the TPR domain interactions | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of Ppp5 in Escherichia coli strain B834 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, under-oil-batch method, 21 mg/ml protein in solution is mixed with an eqal volume of crystallization buffer containing 10% w/v PEG 6000, 4% 2-methyl-2,4-pentanediol, 0.1 M Na-HEPES, pH 8.5, 3 mM DTT, under silicon oil at 14°C, cryoprotection buffer contains 13% w/v PEG 6000, 18% 2-methyl-2,4-pentanediol, 0.075 M NaHEPES, pH 8.5, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modeling | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Ppp5 is inhibited by an autoinhitory conformation formed by the N-terminal TPR domain and a C-terminal subdomain, structure and mechanism overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Ppp5 from Escherichia coli strain B834 by cation exchange, hydrophobic interaction, and nickel affinity chromatography and gel filtration | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate | Glu76 is involved in catalysis, active site structure | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular basis for N-terminal TPR domain-mediated regulation of Ppp5, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Ppp5 | - |
Homo sapiens |
protein phosphatase 5 | - |
Homo sapiens |
serine/threonine phosphatase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |