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Literature summary for 3.1.3.16 extracted from

  • Baldwin, J.C.; Karthikeyan, A.S.; Cao, A.; Raghothama, K.G.
    Biochemical and molecular analysis of LePS2;1: a phosphate starvation induced protein phosphatase gene from tomato (2008), Planta, 228, 273-280.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 P-lysis S cells Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
additional information addition of EGTA and vanadate to the assay does not affect the enzyme activity Solanum lycopersicum
okadaic acid complete inhibition at 50 nM Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information addition of Mg2+ and Ca2+ does not affect the enzyme activity Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
cultivar VFNT Cherry
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RRApTVA + H2O a protein concentration at 15 ng per reaction was found to be optimal for dephosphorylation of the synthetic peptide RRApTVA, maximum enzyme activity can be achieved in the presence of 100 ng of substrate Solanum lycopersicum RRATVA + phosphate
-
?

Synonyms

Synonyms Comment Organism
PS2;1 protein plays an important role in phosphate starvation-induced processes in tomato Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Solanum lycopersicum