Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chlorogenic acid | PP2B but not PP1 is stimulated 4fold by 0.1 mM chlorogenic acid | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
the catalytic subunit of calcineurin is expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the substitution mutant CNA(1-42)-PP1(9-330) has amino acids 1-42 of calcineurin A fused to truncated protein phosphatase 1 with N-terminal residues 1-8 deletion, the mutant is activated by both Mn2+ and Ni2+ and stimulated by chlorogenic acid | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | isozyme PP1 has low activity after depletion of Mn2+ with EDTA | Homo sapiens | |
additional information | protein phosphatase 2B is highly resistant to inhibition by okadaic acid | Homo sapiens | |
okadaic acid | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | isozyme PP1 activity is dependent on Mn2+ with 2.1fold stimulation at 3 mM | Homo sapiens | |
Ni2+ | PP2B but not PP1 is stimulated by Ni2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
protein phosphatase 1, SDS-PAGE | Homo sapiens |
61000 | - |
recombinant catalytic subunit of calcineurin, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
phosphoprotein RII + H2O | - |
Homo sapiens | protein RII + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
calcineurin | - |
Homo sapiens |
CNA | calcineurin catalytic subunit | Homo sapiens |
PP1 | - |
Homo sapiens |
PP1c | protein phosphatase 1 catalytic subunit | Homo sapiens |
PP2B | - |
Homo sapiens |
protein phosphatase 1 | - |
Homo sapiens |
protein phosphatase 2B | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00003 | - |
protein phosphatase 1 | Homo sapiens | okadaic acid | |
0.00012 | - |
mutant enzyme CNA(1-42)-PP1(9-330) | Homo sapiens | okadaic acid | |
0.005 | - |
protein phosphatase 2B | Homo sapiens | okadaic acid |