Cloned (Comment) | Organism |
---|---|
gene prpZ, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Salmonella enterica | |
additional information | no inhibition by okadaic acid | Salmonella enterica | |
Sodium diphosphate | - |
Salmonella enterica | |
Sodium fluoride | - |
Salmonella enterica | |
Sodium phosphate | - |
Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | very high Km for RRLIEDAEY(P)AARG above accurate detection range | Salmonella enterica | |
0.0026 | - |
tyrosine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
0.019 | - |
serine/threonine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
0.13 | - |
RRAT(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
0.24 | - |
RRAS(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
5.7 | - |
4-nitrophenyl phosphate | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, 40fold less effective than Mn2+ at pH 9.5 | Salmonella enterica | |
Mn2+ | activates, 40fold more effective than Mg2+ at pH 9.5 | Salmonella enterica | |
additional information | no effect by Ca2+ and Zn2+, metal ion preference depends strongly on the pH value, overview | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43827 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE, x * 43827, recombinant His-tagged enzyme, mass spectrometry | Salmonella enterica |
51000 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE, x * 43827, recombinant His-tagged enzyme, mass spectrometry | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
serovar Typhi, strains CT18 and Ty2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Salmonella enterica | 4-nitrophenol + phosphate | - |
? | |
additional information | the enzyme preforms dephosphorylation of phosphoserine, phosphothreonine, and also phosphotyrosine residues | Salmonella enterica | ? | - |
? | |
RRAS(P)VA + H2O | synthetic substrate | Salmonella enterica | RRASVA + phosphate | - |
? | |
RRAT(P)VA + H2O | synthetic substrate | Salmonella enterica | RRATVA + phosphate | - |
? | |
RRLIEDAEY(P)AARG + H2O | synthetic substrate | Salmonella enterica | RRLIEDAEYAARG + phosphate | - |
? | |
serine/threonine-phosphorylated myelin basic protein + H2O | - |
Salmonella enterica | myelin basic protein + phosphate | - |
? | |
tyrosine-phosphorylated myelin basic protein + H2O | - |
Salmonella enterica | myelin basic protein + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, recombinant His-tagged enzyme, SDS-PAGE, x * 43827, recombinant His-tagged enzyme, mass spectrometry | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the PPM family, cf. EC 3.1.3.48, protein-tyrosine-phosphatase | Salmonella enterica |
PP2C | - |
Salmonella enterica |
PrpZ | - |
Salmonella enterica |
serine/threonine protein phosphatase 2C | - |
Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
tyrosine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
0.9 | - |
RRAS(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
1.1 | - |
serine/threonine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
2.5 | - |
RRAT(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
7.4 | - |
4-nitrophenyl phosphate | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Salmonella enterica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | - |
Salmonella enterica |