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Enzyme Nomenclature
Information on EC 2.4.2.1 - purine-nucleoside phosphorylase
for references in articles please use BRENDA:EC2.4.2.1
Word Map on EC 2.4.2.1
- 2.4.2.1
- deaminase
- p-nitrophenol
- polynucleotide
- lymphocyte
- hypoxanthine
-
phosphorolysis
- erythrocyte
- rnase
- guanine
-
salvage
- pemphigus
-
paraneoplastic
- immunodeficiency
- t-cells
- phosphoribosyltransferase
- deoxyguanosine
- exoribonuclease
-
pincer
- 5'-nucleotidase
- polyneuropathy
- dgtp
-
practitioner
-
hypoxanthine-guanine
- nurse
-
phosphorylases
- blister
- ribonucleosides
-
degradosome
- 2'-deoxyguanosine
- hgprt
- deoxyadenosine
- deoxycytidine
- deoxynucleosides
-
bullous
-
mucocutaneous
-
castleman
- parathion
-
exonucleolytic
- pemphigoid
- fludarabine
- obliterans
-
desmogleins
-
phrenic
- xanthosine
- medicine
- 2'-deoxyadenosine
-
desmoplakins
-
photocatalytic
- pneumoperitoneum
- synthesis
- lesch-nyhan
- acantholysis
- analysis
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
2.4.2.1
-
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RECOMMENDED NAME
GeneOntology No.
purine-nucleoside phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purine deoxyribonucleoside + phosphate = purine + 2-deoxy-alpha-D-ribose 1-phosphate
(2)
-
-
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
(1)
-
-
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
sequential mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
rapid equilibrium bi bi reaction
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
Theorell-Chance mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
ordered bi-bi mechanism with nucleoside being the first substrate to add and base product to leave
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
rapid equilibrium random bi-bi mechanism with formation of abortive complexes
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
Theorell-Chance mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
sequential bireactant mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
mechanism: 1. random binding of substrates, 2. potent binding and slow release of some reaction products leading to the circumstances that the chamical step is not the slowest one and that rapid-equilibrium assumptions do not hold, 3. dual role of phosphate, as substrate and as reaction modifier
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
steady-state ordered bi bi kinetic mechanism, in which phosphate binds first followed by 2'-deoxyguanosine, and ribose 1-phosphate dissociates first followed by guanine. A general acid is essential for both catalysis and 2'-deoxyguanosine binding, and deprotonation of a group abolishes phosphate binding. Product release contributes to the rate-limiting step
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
purine-nucleoside:phosphate ribosyltransferase
Specificity not completely determined. Can also catalyse ribosyltransferase reactions of the type catalysed by EC 2.4.2.5, nucleoside ribosyltransferase.
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CAS REGISTRY NUMBER
COMMENTARY
9030-21-1
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacterium cadaveris
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-
-
-
489651, 489654, 489670, 489678, 489682, 489683, 489685, 489689, 489694, 489695, 489697, 489709, 489710, 660583, 662986, 663453, 676237, 702230, 703162, 721842, 736851
-
-
-
SwissProt
-
489654, 489655, 489659, 489683, 489688, 489689, 489695, 489700, 489704, 672884, 677014, 686625, 690030, 703006, 721902, 722190, 735581, 735752, 736320
-
-
-
489651, 489654, 489656, 489658, 489664, 489676, 489681, 489684, 489689, 489692, 489705, 489707, 489709, 660818, 663288, 688293, 690065, 701906, 702230, 702231, 702566, 702783, 703162, 703429, 703563, 704899, 704923, 705021, 721554, 722919, 735429, 735691, 736013, 736556, 737172
-
-
-
489711, 660894, 662204, 672251, 672266, 673191, 675064, 675308, 675607, 685103, 685624, 687308, 701905, 702232, 702309, 702365, 702680, 705410, 706562, 706623, 723685
SwissProt
adenine phosphorylase activity and a distinct inosine-guanosine phosphorylase activity
-
-
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
B0L7E7
the salT-deficient mutant strain shows reduced salinosporamide A and salinosporamide B synthesis
metabolism
physiological function
metabolism
B0L7E7
the enzyme is involved in the chloroethylmalonyl-CoA biosynthetic pathway, overview
metabolism
-
comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels
metabolism
-
comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels
physiological function
targeted gene disruption results in loss of enzyme activity in lysate, with normal activity of adenosine deaminase. Mutant parasites show a greater requirement for exogenous purines and a severe growth defect at physiological concentrations of hypoxanthine. The mutant parasites are more sensitive to purine nucleoside phosphorylase inhibitors that bind human purine nucelotide phosphorylase tighter and less sensitive to inhibitor 5'-methylthio-immucillin-H
physiological function
-
parasites lacking purine nucelotide phosphorylase due to gene disruption are attenuated and clear in mice. Although able to form gametocytes, purine nucelotide phosphorylase-deficient parasites do not form oocysts in mosquito midguts and are not transmitted from mosquitoes to mice. Mice given purine nucelotide phosphorylase-deficient parasites are immune to subsequent challenge to a lethal inoculum of Plasmodium yoelii YM and to challenge from strain Plasmodium yoelii 17XNL
physiological function
-
the enzyme is active in tubers as a negative regulator of cytokinins, prolonging endodormancy by a chill-reversible mechanism
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,6-dihydropurine riboside + phosphate
alpha-D-ribose 1-phosphate + 1,6-dihydropurine
-
-
-
-
?
1-methylguanosine + phosphate
alpha-D-ribose 1-phosphate + 1-methylguanine
-
-
-
-
?
1-methylinosine + phosphate
alpha-D-ribose 1-phosphate + 1-methylhypoxanthine
-
-
-
-
?
2',3'-dideoxyinosine + phosphate
hypoxanthine + 2,3-dideoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2',3'-dideoxyinosine + phosphate
hypoxanthine + alpha-D-2,3-dideoxyribose 1-phosphate
-
-
-
-
?
2'-amino-2'-deoxyadenosine + phosphate
adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
2'-amino-2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxy-2'-fluoroadenosine + phosphate
adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate
-
-
-
-
?
2'-deoxy-6-methylpurine nucleoside + phosphate
?
13.2% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
2-deoxy-alpha-D-ribose 1-phosphate + adenine
-
10% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + alpha-D-2-deoxyribose 1-phosphate
-
-
-
-
?
2'-deoxyinosine + phosphate
2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine
-
-
-
-
?
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-ribosyl-2,6-diamino-8-azapurine + N7-ribosyl-2,6-diamino-8-azapurine + phosphate
-
-
-
-
?
2,6-diaminopurine nucleoside + phosphate
alpha-D-ribose 1-phosphate + 2,6-diaminopurine
-
-
-
-
2,6-diaminopurine ribonucleoside + phosphate
2,6-diaminopurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + ?
-
-
-
-
?
2-chloroadenosine + phosphate
2-chloroadenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + 2-deoxy-alpha-D-ribose 1-phosphate
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + alpha-D-ribose 1-phosphate
-
no substrate for wild-type, substrate of double mutant E201Q/N243D
-
-
?
2-fluoroadenosine + phosphate
2-fluoroadenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-chloro-5'-deoxy-adenosine + phosphate
5-chloro-5-deoxy-D-ribose 1-phosphate + adenine
B0L7E7
-
-
-
-
5'-deoxy-5'-fluoroadenosine + phosphate
5-deoxy-5-fluoro-D-ribose-1-phosphate + adenine
-
5'-deoxy-5'-fluoroadenosine + phosphate i.e. 5'-FDA
5-deoxy-5-fluoro-D-ribose-1-phosphate i.e. 5-FDRP
-
?
5'-deoxy-5'-iodo-2-fluoroadenosine + phosphate
2-fluoroadenine + 5-deoxy-5-iodo-alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
6-(2-thienyl)-9-(beta-D-ribofuranosyl)purine + phosphate
6-(2-thienyl)purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-cyclopropyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-cyclopropylpurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-ethyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-ethylpurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-mercaptoguanosine + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptoguanine
-
-
-
-
?
6-phenyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-phenylpurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7,8-dimethylguanosine + phosphate
7,8-dimethylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-butylguanosine + phosphate
7-butylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-ethylguanosine + phosphate
7-ethylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-isobutylguanosine + phosphate
7-isobutylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-isopropylguanosine + phosphate
7-isopropylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyl-6-thio-guanosine + phosphate
7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyl-8-aminoguanosine + phosphate
7-methyl-8-aminoguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-prolylguanosine + phosphate
7-prolylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
N9-beta-D-ribosyl-8-azaguanine + phosphate
-
-
-
-
?
8-azahypoxanthine + alpha-D-ribose 1-phosphate
8-azainosine + phosphate
-
-
-
?
8-methylguanosine + phosphate
8-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
9-(2-deoxy-2-fluoro-alpha-D-arabinofuranosyl)adenine + phosphate
adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate
-
-
-
-
?
9-(6-deoxy-5-C-methyl-beta-D-ribo-hexofuranosyl)-6-methylpurine + phosphate
?
-
-
-
-
r
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine + phosphate
6-methylthiopurine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
9-beta-D-ribofuranosyl-6-methylthiopurine + phosphate
6-methylthiopurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug
-
-
?
adenine arabinoside + phosphate
alpha-D-arabinose 1-phosphate + adenine
-
49% of the activity with adenosine
-
-
-
adenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
activity with mutant enzyme N239D, wild-type and mutant enzyme Y191L show no activity
-
-
?
arabinofuranosyladenine + phosphate
?
4.3% of the activity with inosine
-
-
?
cytokinin + phosphate
? + alpha-D-ribose 1-phosphate
-
the enzyme prefers cytokinin to unsubstituted aminopurines
-
-
r
dihydrozeatin riboside + phosphate
dihydrozeatin + alpha-D-ribose 1-phosphate
-
-
-
-
r
isopentenyladenosine + phosphate
isopentenyladenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
more '
?
-
catalysis involves protonation of the purine base at position N7 by residue Asp204, which is triggered by Arg217
-
-
-
ribavirin + phosphate
1,2,4-triazole-3-carboxamide + alpha-D-ribose 1-phosphate
-
-
-
-
?
ribavirin + phosphate
alpha-D-ribose 1-phosphate + 1,2,4-triazole-3-carboxamide
-
22% of the activity with inosine. Phosphorolysis reaches an equilibrium when 15% of ribavirin is phosphorolyzed
-
-
r
trans-zeatin riboside + phosphate
trans-zeatin + alpha-D-ribose 1-phosphate
-
-
-
-
r
2',3'-dideoxyinosine + phosphate
alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
22% of the activity with inosine
-
-
?
2',3'-dideoxyinosine + phosphate
alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
12% of the activity with inosine
-
-
?
2'-amino-2'-deoxyadenosine + phosphate
adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-amino-2'-deoxyadenosine + phosphate
adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
12% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
54.7% of the activity with 2'-deoxyinosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
13.4% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
37% activity compared to adenosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
20% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
83.2% of the activity with 2'-deoxyinosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
3.7% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
22.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
22.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 23% of the activity with inosine, isoform of 702 amino acids, 31% of the activity with inosine
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
22% of the activity with inosine
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
40.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
40.5% phosphorolysis of substrate within 30 min
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
-
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
the product 2-fluoroadenine is highly cytotoxic for Trichomonas vaginalis
-
?
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
-
-
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
-
?
3-deazainosine + phosphate
alpha-D-ribose 1-phosphate + 3-deazahypoxanthine
-
-
-
-
?
3-deazainosine + phosphate
alpha-D-ribose 1-phosphate + 3-deazahypoxanthine
-
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
-
40% activity compared to adenosine
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
-
r
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
ver low activity
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
very low activity
-
-
?
6-mercaptopurine riboside + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptopurine
-
-
-
-
-
6-mercaptopurine riboside + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptopurine
-
14% of the activity with inosine
-
-
?
6-thioxanthine + alpha-D-ribose 1-phosphate
6-thioxanthosine + phosphate
-
-
-
-
?
6-thioxanthine + alpha-D-ribose 1-phosphate
6-thioxanthosine + phosphate
-
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
-
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
-
-
-
-
?
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, liberation of methylpurine
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V
-
-
?
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
-
-
-
-
-
adenosine + arsenate
adenine + alpha-D-ribose 1-arsenate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
61% of the activity with deoxyguanosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
41% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
59.9% of the activity with 2'-deoxyinosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
160% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
activity with OUNOII, no activity with PUNPI
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
160% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
best substrate
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
best substrate
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
wild-type enzyme and mutant enzyme K244Q show no activity with adenosine, mutant enzyme N243D and N243D/K244Q are active with adenosine
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
15.4% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
45% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
78% of the activity with deoxyguanosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
100% activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
exclusive substrate for the hexameric enzyme
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
exclusive substrate for the hexameric enzyme
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
in the reverse reaction the catalytic activity with adenine is higher than that with either hypoxanthine or guanine
-
-
r
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
112.9% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
112.9% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
61% of the activity with deoxyguanosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 160% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 57% of the activity with inosine. 3'-deoxyadenosine, 7% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
78% of the activity with deoxyguanosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
reaction with ionosine-guanosine phosphorylase, very low activity with the adenosine-specific phosphorylase
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
11% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 469% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
85% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 469% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
85% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
-
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
14% of the activity with inosine
-
-
?
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
19% of the activity with inosine
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine and 5'-deoxyinosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
88% of the activity with deoxyguanosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 87% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
as active as inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 691% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 691% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
as active as inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 43% of the activity with adenosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 43% of the activity with adenosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
136% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
136% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
-
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
88% of the activity with deoxyguanosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
guanosine + arsenate
guanine + alpha-D-ribose 1-arsenate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
no activity
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
47.4% of the reaction with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
47.4% of the reaction with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
48% of the activity with deoxyguanosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
no activity with mutant enzyme N239D
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
58.7% of the activity with 2'-deoxyinosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
66% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
21% of the activity with inosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
53% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
84% of the activity with guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
53% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
84% of the activity with guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
insight into catalytically relevant correlated motions in human purine nucleoside phosphorylase
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
2.3% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
55.8% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
56% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
absolute specificity for inosine and guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
16% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
81% of the activity with deoxyguanosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
8.7% phosphorolysis of substrate within 30 min
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
exclusive substrate for the trimeric enzyme
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
8.7% phosphorolysis of substrate within 30 min
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
exclusive substrate for the trimeric enzyme
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + arsenate
hypoxanthine + ribose 1-arsenate
-
ribosyl group is nearly dissociated from the base prior to attack of the arsenate
-
?
inosine + arsenate
hypoxanthine + ribose 1-arsenate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
no activity
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
reaction with ionosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
the equilibrium is reached when approximately 14% of inosine is phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
the equilibrium is reached when approximately 14% of inosine is phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
highest activity
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
46% of the activity with deoxyguanosine
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
83% of the activity with 2'-deoxyinosine
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
reaction reaches an equilibrium when 21% of the inosine is phosphorolyzed
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
34% of the activity with adenosine
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
equilibrium when 65% of inosine has been phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
34% of the activity with adenosine
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
equilibrium when 65% of inosine has been phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
C8CPR9
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
C8CPR9
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
absolute specificity for inosine and guanosine
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
82% of the activity with deoxyguanosine
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
19.1% phosphorolysis of substrate within 30 min
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
19.1% phosphorolysis of substrate within 30 min
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
-
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
43.5% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
43.5% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
no activity with mutant enzymes Y191L and N239D
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
26% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
reaction only at high concentrations
-
-
?
additional information
?
-
the enzyme cannot accept 6-chloropurines
-
-
-
additional information
?
-
the enzyme cannot accept 6-chloropurines
-
-
-
additional information
?
-
no activity is detected for adenosine or 2'-deoxyadenosine
-
-
-
additional information
?
-
-
no activity is detected for adenosine or 2'-deoxyadenosine
-
-
-
additional information
?
-
-
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-
-
-
additional information
?
-
-
enzyme shows a high tolerance to the steric and hydrophobic environment at the 6-position of the synthesized purine ribonucleosides
-
-
-
additional information
?
-
-
no substrate: xanthosine, uridine, 2'-deoxythymidine, cytidine
-
-
-
additional information
?
-
-
cellular function seems concerned primarily with nucleoside breakdown
-
-
-
additional information
?
-
-
children lacking PNP activity exhibit severe T cell immunodeficiency while maintaining normal or exaggerated B cell function. The PNP deficiency results in very low uric acid concentrations and high concentrations of the nucleoside substrate of PNP in plasma and urine
-
-
-
additional information
?
-
in intact cells, the enzyme functions in the direction of phosphorolysis, leading to degradation of purine nucleosides via coupling with guanase and xanthine oxidase. PNP deficiency is a rare disorder associated with an autosomal recessive form of cellular, but not humoral, immunodeficiency, and comprises about 4% of all cases of severe combined immunodeficiency. Mutations identified in cases of PNP deficiency
-
-
-
additional information
?
-
enzyme plays a key role in the purine salvage pathway. PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function
-
-
-
additional information
?
-
Leucisus rusticus
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
-
additional information
?
-
-
enzyme is more specific to natural 6-oxopurine nucleosides and synthetic compounds, and does not catalyze the phosphorolysis of adenosine
-
-
-
additional information
?
-
C8CPR9
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
-
additional information
?
-
-
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
-
additional information
?
-
C8CPR9
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
-
additional information
?
-
-
enzyme is able to reactivate scrambled RNaseA
-
-
-
additional information
?
-
salmon
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
-
additional information
?
-
-
the enzyme is specific for adenine nucleosides which includes adenosine analogs modified in the aglycone, pentose or both moieties
-
-
-
additional information
?
-
-
no activity with inosine and guanosine
-
-
-
additional information
?
-
-
the enzyme has no detectable ribohydrolase activity
-
-
-
additional information
?
-
no substrate: acyclovir, benzimidazole, 2,6-diaminopurine
-
-
-
additional information
?
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
no substrate: acyclovir, benzimidazole, 2,6-diaminopurine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
Q97W94
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
Q97W94
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
additional information
?
-
-
cellular function seems concerned primarily with nucleoside breakdown
-
-
-
additional information
?
-
-
children lacking PNP activity exhibit severe T cell immunodeficiency while maintaining normal or exaggerated B cell function. The PNP deficiency results in very low uric acid concentrations and high concentrations of the nucleoside substrate of PNP in plasma and urine
-
-
-
additional information
?
-
P00491
in intact cells, the enzyme functions in the direction of phosphorolysis, leading to degradation of purine nucleosides via coupling with guanase and xanthine oxidase. PNP deficiency is a rare disorder associated with an autosomal recessive form of cellular, but not humoral, immunodeficiency, and comprises about 4% of all cases of severe combined immunodeficiency. Mutations identified in cases of PNP deficiency
-
-
-
additional information
?
-
P00491
enzyme plays a key role in the purine salvage pathway. PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function
-
-
-
additional information
?
-
Leucisus rusticus
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
-
additional information
?
-
salmon
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
-
additional information
?
-
-
the enzyme is specific for adenine nucleosides which includes adenosine analogs modified in the aglycone, pentose or both moieties
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
with (hypoxanthine-9-yl)methyl aglycone, i.e. Yokomatsu compound
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranyl)ethylphosphonic acid with (hypoxanthine-9-yl)methyl aglycone
(3R,4R)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
-
(3S)-3-(2-amino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-(3-chlorophenyl)propanoic acid
-
IC50: 0.000036 mM
(3S,4R)-4-(guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylcarbonylphosphonic acid
-
competitive. For enzyme from cancer cell lines, Ki values are around 10 to 24 nM
(3S,4S)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
-
(S)-3-(guanin-9-yl)pyrrolidin-N-ylcarbonylphosphonic acid
-
competitive. For enzyme from cancer cell lines, Ki values widely vary from 16 to 100 nM
1-((2-pyrrolidine-1-yl)ethyl)uracil
inhibits both enzymic activity and growth of Plasmodium falciparum
1-[4-(2,4-dimethylphenyl)-1,3-thiazol-2-yl]guanidine
-
0.1 mM , 79.5% inhibition
1-[4-(4-methylphenyl)-1,3-thiazol-2-yl]guanidine
-
0.1 mM , 70.6% inhibition
2,6-diamino-7-(5-mercapto-3,3-dimethylpentyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00005 mM
2,6-diamino-7-(cyclohexylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.0066 mM
2,8-diamino-9-(1,3-thiazol-4-ylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.001043 mM
2,8-diamino-9-(2-furylmethyl)-1,9-dihydro-6H-purin-6-one