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Literature summary for 2.4.2.1 extracted from

  • Visser, D.F.; Hennessy, F.; Rashamuse, K.; Louw, M.E.; Brady, D.
    Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 (2010), Extremophiles, 14, 185-192.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Halalkalibacterium halodurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.206
-
 guanosine pH 7.5 Halalkalibacterium halodurans
0.236
-
 Inosine pH 7.5 Halalkalibacterium halodurans

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans C7FLN0
-
-
Halalkalibacterium halodurans Alk36 C7FLN0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Halalkalibacterium halodurans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.23
-
 pH 7.5, substrate inosine Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine + phosphate
-
 Halalkalibacterium halodurans guanine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Halalkalibacterium halodurans Alk36 guanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Halalkalibacterium halodurans hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Halalkalibacterium halodurans Alk36 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Halalkalibacterium halodurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32 74 more than 60% of maximum activity Halalkalibacterium halodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life 20.8 h Halalkalibacterium halodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
 guanosine pH 7.5 Halalkalibacterium halodurans
28000
-
 Inosine pH 7.5 Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Halalkalibacterium halodurans

pH Range

pH Minimum pH Maximum Comment Organism
5.7 8.4
-
 Halalkalibacterium halodurans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
58.7
-
 guanosine pH 7.5 Halalkalibacterium halodurans
120000
-
 Inosine pH 7.5 Halalkalibacterium halodurans