Protein Variants | Comment | Organism |
---|---|---|
L22E/H104R | mutation of human toward bovine purine nucleoside phosphorylase. Mutant is similar to human purine nucleoside phosphorylase in steady-state kinetic properties. Inhibitor DADMe-immucillin-G is an excellent mimic of the transition states for both human purine nucleoside phosphorylase and the mutant with a preference for the mutant. Thermodynamic parameters establish the mutant to be catalytically more efficient than the parent enzyme and reveal differences in the entropic component of catalysis | Homo sapiens |
L22E/H104R | mutation of human toward bovine purine nucleoside phosphorylase. Mutant is similar to human purine nucleoside phosphorylase in steady-state kinetic properties. Inhibitor DADMe-immucillin-G is an excellent mimic of the transition states for both human purine nucleoside phosphorylase and the mutant with a preference for the mutant. Thermodynamic parameters establish the mutant to be catalytically more efficient than the parent enzyme and reveal differences in the entropic component of catalysis | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DADMe-immucillin-G | - |
Homo sapiens | |
DADMe-immucillin-H | - |
Homo sapiens | |
immucillin-G | - |
Homo sapiens | |
immucillin-H | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
Inosine | pH 7.4, 25°C | Bos taurus | |
0.027 | - |
Inosine | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
0.03 | - |
Inosine | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
0.042 | - |
guanosine | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
0.047 | - |
guanosine | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
0.049 | - |
guanosine | pH 7.4, 25°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine + phosphate | - |
Homo sapiens | guanine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Bos taurus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
Inosine | pH 7.4, 25°C | Bos taurus | |
27 | - |
guanosine | pH 7.4, 25°C | Bos taurus | |
30 | - |
guanosine | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
31 | - |
guanosine | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
31.5 | - |
Inosine | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
40 | - |
Inosine | wild-tpe, pH 7.4, 25°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000034 | - |
DADMe-immucillin-G | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
0.000000121 | - |
DADMe-immucillin-G | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
0.000000133 | - |
immucillin-G | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
0.000000175 | - |
immucillin-G | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
0.00000024 | - |
DADMe-immucillin-H | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
0.00000035 | - |
DADMe-immucillin-H | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
0.00000067 | - |
immucillin-H | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
0.000001 | - |
immucillin-H | wild-tpe, pH 7.4, 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
550 | - |
guanosine | pH 7.4, 25°C | Bos taurus | |
630 | - |
guanosine | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
730 | - |
guanosine | wild-tpe, pH 7.4, 25°C | Homo sapiens | |
1200 | - |
Inosine | mutant L22E/H104R, pH 7.4, 25°C | Homo sapiens | |
1250 | - |
Inosine | pH 7.4, 25°C | Bos taurus | |
1300 | - |
Inosine | wild-tpe, pH 7.4, 25°C | Homo sapiens |