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Literature summary for 2.4.2.1 extracted from

  • Ghanem, M.; Li, L.; Wing, C.; Schramm, V.L.
    Altered thermodynamics from remote mutations altering human toward bovine purine nucleoside phosphorylase (2008), Biochemistry, 47, 2559-2564.
    View publication on PubMed
Search for more literature for 2.4.2.1

Protein Variants

Protein Variants Comment Organism
L22E/H104R mutation of human toward bovine purine nucleoside phosphorylase. Mutant is similar to human purine nucleoside phosphorylase in steady-state kinetic properties. Inhibitor DADMe-immucillin-G is an excellent mimic of the transition states for both human purine nucleoside phosphorylase and the mutant with a preference for the mutant. Thermodynamic parameters establish the mutant to be catalytically more efficient than the parent enzyme and reveal differences in the entropic component of catalysis Homo sapiens
L22E/H104R mutation of human toward bovine purine nucleoside phosphorylase. Mutant is similar to human purine nucleoside phosphorylase in steady-state kinetic properties. Inhibitor DADMe-immucillin-G is an excellent mimic of the transition states for both human purine nucleoside phosphorylase and the mutant with a preference for the mutant. Thermodynamic parameters establish the mutant to be catalytically more efficient than the parent enzyme and reveal differences in the entropic component of catalysis Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
DADMe-immucillin-G
-
 Homo sapiens
DADMe-immucillin-H
-
 Homo sapiens
immucillin-G
-
 Homo sapiens
immucillin-H
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 Inosine pH 7.4, 25°C Bos taurus
0.027
-
 Inosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
0.03
-
 Inosine wild-tpe, pH 7.4, 25°C Homo sapiens
0.042
-
 guanosine wild-tpe, pH 7.4, 25°C Homo sapiens
0.047
-
 guanosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
0.049
-
 guanosine pH 7.4, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine + phosphate
-
 Homo sapiens guanine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Bos taurus guanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 Inosine pH 7.4, 25°C Bos taurus
27
-
 guanosine pH 7.4, 25°C Bos taurus
30
-
 guanosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
31
-
 guanosine wild-tpe, pH 7.4, 25°C Homo sapiens
31.5
-
 Inosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
40
-
 Inosine wild-tpe, pH 7.4, 25°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000034
-
 DADMe-immucillin-G wild-tpe, pH 7.4, 25°C Homo sapiens
0.000000121
-
 DADMe-immucillin-G mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
0.000000133
-
 immucillin-G wild-tpe, pH 7.4, 25°C Homo sapiens
0.000000175
-
 immucillin-G mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
0.00000024
-
 DADMe-immucillin-H mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
0.00000035
-
 DADMe-immucillin-H wild-tpe, pH 7.4, 25°C Homo sapiens
0.00000067
-
 immucillin-H mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
0.000001
-
 immucillin-H wild-tpe, pH 7.4, 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
550
-
 guanosine pH 7.4, 25°C Bos taurus
630
-
 guanosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
730
-
 guanosine wild-tpe, pH 7.4, 25°C Homo sapiens
1200
-
 Inosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
1250
-
 Inosine pH 7.4, 25°C Bos taurus
1300
-
 Inosine wild-tpe, pH 7.4, 25°C Homo sapiens