Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | no inhibition of PUNPII | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
adenosine | - |
Geobacillus stearothermophilus | |
0.19 | - |
2'-deoxyadenosine | - |
Geobacillus stearothermophilus | |
0.22 | - |
Inosine | - |
Geobacillus stearothermophilus | |
0.34 | - |
guanosine | - |
Geobacillus stearothermophilus | |
0.92 | - |
2'-deoxyinosine | - |
Geobacillus stearothermophilus | |
1.9 | - |
2'-deoxyguanosine | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
JTS 859 | - |
Geobacillus stearothermophilus JTS 859 | - |
JTS 859 | - |
Purification (Comment) | Organism |
---|---|
PUNPI and PUNPII | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.6 | - |
PUNPII | Geobacillus stearothermophilus |
405 | - |
PUNPI | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + phosphate | 160% of the activity with inosine | Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | activity with OUNOII, no activity with PUNPI | Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | 160% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
deoxyadenosine + phosphate | 2'-deoxyadenosine, 160% of the activity with inosine | Geobacillus stearothermophilus | alpha-D-ribose 1-phosphate + adenine | - |
? | |
deoxyguanosine + phosphate | 2'-deoxyguanosine, 469% of the activity with inosine | Geobacillus stearothermophilus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyguanosine + phosphate | 2'-deoxyguanosine, 469% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyinosine + phosphate | 2'-deoxyinosine, 691% of the activity with inosine | Geobacillus stearothermophilus | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
deoxyinosine + phosphate | 2'-deoxyinosine, 691% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
guanosine + phosphate | 53% of the activity with inosine | Geobacillus stearothermophilus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | 53% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life of isoenzyme PUNPII is 15 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11.5 | pH 5.0: about 50% of maximal activity, pH 11.5: about 85% of maximal activity | Geobacillus stearothermophilus |