Crystallization (Comment) | Organism |
---|---|
enzyme in complex with the inhibitor 5'-methylthio-immucillin-H, hanging-drop vapor-diffusion method | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthio-immucillin-H | favors inhibition of Plasmodium falciparum purine nucleoside phosphorylase over human enzyme | Homo sapiens | |
5'-methylthio-immucillin-H | favours inhibition of Plasmodium falciparum purine nucleoside phosphorylase over human enzyme | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
Inosine | pH 7.5, 25°C | Plasmodium falciparum | |
0.012 | - |
Methylthioinosine | pH 7.5, 25°C | Homo sapiens | |
0.016 | - |
Methylthioinosine | pH 7.5, 25°C | Plasmodium falciparum | |
0.038 | - |
Inosine | pH 7.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00491 | - |
- |
Plasmodium falciparum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioinosine + phosphate | - |
Homo sapiens | hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
methylthioinosine + phosphate | - |
Plasmodium falciparum | methylthiohypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
methylthioinosine + phosphate | - |
Homo sapiens | methylthiohypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Methylthioinosine | pH 7.5, 25°C | Homo sapiens | |
1.5 | - |
Methylthioinosine | pH 7.5, 25°C | Plasmodium falciparum | |
1.7 | - |
Inosine | pH 7.5, 25°C | Plasmodium falciparum | |
31 | - |
Inosine | pH 7.5, 25°C | Homo sapiens |