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Literature summary for 2.4.2.1 extracted from

  • Jensen, K.J.
    Two purine nucleoside phosphorylases in Bacillus subtilis. Purification and some properties of the adenosine-specific phosphorylase (1978), Biochim. Biophys. Acta, 525, 346-356.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
guanosine strong competitive inhibitor with deoxyinosine as substrate Bacillus subtilis
Inosine strong competitive inhibitor with deoxyinosine as substrate Bacillus subtilis
PCMB adenosine-specific phosphorylase Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
Inosine inosine-guanosine phosphorylase Bacillus subtilis
3.9
-
phosphate reaction with inosine, inosine-guanosine phosphorylase Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
6 * 25500, adenosine-specific phosphorylase, SDS-PAGE Bacillus subtilis
28000
-
x * 28000, inosine-guanosine phosphorylase, SDS-PAGE Bacillus subtilis
95000
-
inosine-guanosine phosphoylase, gel filtration Bacillus subtilis
153000
-
adenosine-specific phosphorylase, gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-

Purification (Commentary)

Purification (Comment) Organism
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73
-
adenosine-specific phosphorylase Bacillus subtilis
100
-
inosine-guanosine phosphorylase Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + phosphate reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase Bacillus subtilis adenine + alpha-D-ribose 1-phosphate
-
?
deoxyadenosine + phosphate reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase Bacillus subtilis alpha-D-ribose 1-phosphate + adenine
-
?
deoxyguanosine + phosphate reaction with ionosine-guanosine phosphorylase, very low activity with the adenosine-specific phosphorylase Bacillus subtilis guanine + alpha-D-deoxyribose 1-phosphate
-
?
deoxyinosine + phosphate reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase Bacillus subtilis hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
guanosine + phosphate reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase Bacillus subtilis guanine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate reaction with ionosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase Bacillus subtilis hypoxanthine + alpha-D-ribose 1-phosphate
-
?
additional information two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
? x * 28000, inosine-guanosine phosphorylase, SDS-PAGE Bacillus subtilis
hexamer 6 * 25500, adenosine-specific phosphorylase, SDS-PAGE Bacillus subtilis