Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanosine | strong competitive inhibitor with deoxyinosine as substrate | Bacillus subtilis | |
Inosine | strong competitive inhibitor with deoxyinosine as substrate | Bacillus subtilis | |
PCMB | adenosine-specific phosphorylase | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Inosine | inosine-guanosine phosphorylase | Bacillus subtilis | |
3.9 | - |
phosphate | reaction with inosine, inosine-guanosine phosphorylase | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
6 * 25500, adenosine-specific phosphorylase, SDS-PAGE | Bacillus subtilis |
28000 | - |
x * 28000, inosine-guanosine phosphorylase, SDS-PAGE | Bacillus subtilis |
95000 | - |
inosine-guanosine phosphoylase, gel filtration | Bacillus subtilis |
153000 | - |
adenosine-specific phosphorylase, gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase | - |
Purification (Comment) | Organism |
---|---|
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73 | - |
adenosine-specific phosphorylase | Bacillus subtilis |
100 | - |
inosine-guanosine phosphorylase | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + phosphate | reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase | Bacillus subtilis | adenine + alpha-D-ribose 1-phosphate | - |
? | |
deoxyadenosine + phosphate | reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase | Bacillus subtilis | alpha-D-ribose 1-phosphate + adenine | - |
? | |
deoxyguanosine + phosphate | reaction with ionosine-guanosine phosphorylase, very low activity with the adenosine-specific phosphorylase | Bacillus subtilis | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyinosine + phosphate | reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase | Bacillus subtilis | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
guanosine + phosphate | reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase | Bacillus subtilis | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | reaction with ionosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase | Bacillus subtilis | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
additional information | two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, inosine-guanosine phosphorylase, SDS-PAGE | Bacillus subtilis |
hexamer | 6 * 25500, adenosine-specific phosphorylase, SDS-PAGE | Bacillus subtilis |