Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.1 extracted from

  • Breer, K.; Girstun, A.; Wielgus-Kutrowska, B.; Staro?, K.; Bzowska, A.
    Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP) (2008), Protein Expr. Purif., 61, 122-130.
    View publication on PubMed
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
 Bos taurus
guanine
-
 Bos taurus
hypoxanthine
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
 Inosine pH 7.0, 25°C, recombinant enzyme Bos taurus
0.0168
-
 7-methylguanosine pH 7.0, 25°C, recombinant enzyme Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P55859
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0307
-
 inosine as a variable substrate Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylguanosine + phosphate
-
 Bos taurus 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PNP
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000033
-
 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine pH 7.0, 25°C, in the presence of 1 mM phosphate Bos taurus
0.0016
-
 hypoxanthine pH 7.0, 25°C Bos taurus
0.0025
-
 guanine pH 7.0, 25°C Bos taurus