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Literature summary for 2.4.2.1 extracted from
- Characterization of the N6-etheno-bridge method to assess extracellular metabolism of adenine nucleotides detection of a possible role for purine nucleoside phosphorylase in adenosine metabolism (2020), Purinergic Signal., 16, 187-211 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| forodesine | - |
Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | ? | - |
- |
|
| additional information | Rattus norvegicus Wistar-Kyoto | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00491 | - |
- |
| Rattus norvegicus | P85973 | - |
- |
| Rattus norvegicus Wistar-Kyoto | P85973 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | recombinant enzyme | Homo sapiens | - |
| fibroblast | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| mesangial cell | - |
Rattus norvegicus | - |
| vascular smooth muscle cell | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-methyl-6-thioguanosine + phosphate | - |
Homo sapiens | 7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | complete conversion | Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| additional information | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | Rattus norvegicus | ? | - |
- |
|
| additional information | the recombinant enzyme does not metabolize adenosine to adenine or N6-etheno-adenosine to N6-etheno-adenine | Homo sapiens | ? | - |
- |
|
| additional information | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | Rattus norvegicus Wistar-Kyoto | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | the commercial recombinant enzyme is in monomeric form | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNPase | - |
Homo sapiens |
| PNPase | - |
Rattus norvegicus |
| purine nucleoside phosphorylase | - |
Homo sapiens |
| purine nucleoside phosphorylase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | N6-etheno-ATP and its downstream metabolites are devoid of pharmacological activity | Rattus norvegicus |
| physiological function | PNP catalyzes a reversible phosphorolysis of the N-glycosidic bond in natural purine nucleosides, as well as inmany purine analogues, some of them displaying marked biological and pharmacological activity | Homo sapiens |
| physiological function | PNP catalyzes a reversible phosphorolysis of the N-glycosidic bond in natural purine nucleosides, as well as inmany purine analogues, some of them displaying marked biological and pharmacological activity | Rattus norvegicus |
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