Crystallization (Comment) | Organism |
---|---|
crystal structures of the TvPNP/immucillin-A/PO4 and TvPNP/4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A/PO4 ternary complexes | Trichomonas vaginalis |
crystal structures of the TvPNP/immucillin-A/PO4 and TvPNP/deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A /PO4 ternary complexes. | Trichomonas vaginalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | weak binding inhibitor | Homo sapiens | |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | weak binding inhibitor | Plasmodium falciparum | |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | dissociation constant of 30 pM, an inhibitor release half-time of 64 min. Tight binding with 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A is in part due to a 2.7 A ionic interaction between a PO4 oxygen and the N1' cation of the hydroxypyrrolidine. The interaction of the inhibitor with TvPNP is the tightest-binding enzymatic interaction known for an immucillin analogue of adenosine; tight binding inhibitor | Trichomonas vaginalis | |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-G | - |
Homo sapiens | |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-G | - |
Trichomonas vaginalis | |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | - |
Trichomonas vaginalis | |
immucillin-A | weak binding inhibitor | Homo sapiens | |
immucillin-A | weak binding inhibitor | Plasmodium falciparum | |
immucillin-A | slow-onset tight binding inhibition, inhibitor release half-time of 17.2 min; slow-onset tightbinding inhibition with TvPNP, to give an equilibrium dissociation constant of 87 pM and an inhibitor release half-time of 17.2 min | Trichomonas vaginalis | |
immucillin-G | dissociation constant of 42 pM | Homo sapiens | |
immucillin-G | dissociation constant of 900 pM | Plasmodium falciparum | |
immucillin-G | - |
Trichomonas vaginalis | |
immucillin-H | dissociation constant: 23 pM | Bos taurus | |
immucillin-H | dissociation constant: 56 pM | Homo sapiens | |
immucillin-H | - |
Trichomonas vaginalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
adenosine | - |
Trichomonas vaginalis | |
0.0061 | - |
adenosine | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.025 | - |
Inosine | - |
Trichomonas vaginalis | |
0.025 | - |
Inosine | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.06 | - |
guanosine | - |
Trichomonas vaginalis | |
0.06 | - |
guanosine | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.09 | - |
p-nitrophenyl beta-D-ribofuranoside | - |
Trichomonas vaginalis | |
0.09 | - |
p-nitrophenyl beta-D-ribofuranoside | 25°C, pH 7.4 | Trichomonas vaginalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P55859 | - |
- |
Homo sapiens | P00491 | - |
- |
Plasmodium falciparum | Q8T9Z7 | - |
- |
Trichomonas vaginalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + phosphate | - |
Trichomonas vaginalis | adenine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Trichomonas vaginalis | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Trichomonas vaginalis | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
p-nitrophenyl beta-D-ribofuranoside + phosphate | - |
Trichomonas vaginalis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HsPNP | - |
Homo sapiens |
TvPNP | - |
Trichomonas vaginalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
p-nitrophenyl beta-D-ribofuranoside | 25°C, pH 7.4 | Trichomonas vaginalis | |
1 | - |
p-nitrophenyl beta-D-ribofuranoside | - |
Trichomonas vaginalis | |
1.1 | - |
guanosine | - |
Trichomonas vaginalis | |
1.1 | - |
guanosine | 25°C, pH 7.4 | Trichomonas vaginalis | |
1.7 | - |
adenosine | - |
Trichomonas vaginalis | |
1.7 | - |
adenosine | 25°C, pH 7.4 | Trichomonas vaginalis | |
1.8 | - |
Inosine | - |
Trichomonas vaginalis | |
1.8 | - |
Inosine | 25°C, pH 7.4 | Trichomonas vaginalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
immucillin-A | - |
Trichomonas vaginalis | |
0.000001 | - |
immucillin-A | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.0000015 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Trichomonas vaginalis | |
0.0000015 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.0000038 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | - |
Trichomonas vaginalis | |
0.0000038 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.000012 | - |
immucillin-H | - |
Trichomonas vaginalis | |
0.000012 | - |
immucillin-H | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.000016 | - |
immucillin-G | - |
Trichomonas vaginalis | |
0.000016 | - |
immucillin-G | 25°C, pH 7.4 | Trichomonas vaginalis | |
0.000018 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-G | - |
Trichomonas vaginalis | |
0.000018 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | 25°C, pH 7.4 | Trichomonas vaginalis |