Protein Variants | Comment | Organism |
---|---|---|
additional information | targeted gene disruption results in loss of enzyme activity in lysate, with normal activity of adenosine deaminase. Mutant parasites show a greater requirement for exogenous purines and a severe growth defect at physiological concentrations of hypoxanthine. The mutant parasites are more sensitive to purine nucelotide phosphorylase inhibitors that bind human purine nucelotide phosphorylase tighter and less sensitive to inhibitor 5'-methylthio-immucillin-H | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-immucillin-G | - |
Plasmodium falciparum | |
2'-deoxy-immucillin-H | - |
Plasmodium falciparum | |
5'-methylthio-immucillin-H | - |
Plasmodium falciparum | |
immucillin-H | - |
Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8T9Z7 | 3D7 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.016 | - |
substrate 5'-methylthioinosine, pH 7.4 | Plasmodium falciparum |
0.023 | - |
substrate inosine, pH 7.4 | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioinosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000064 | - |
gene disruption mutant, pH 7.4 | Plasmodium falciparum | 2'-deoxy-immucillin-G | |
0.0000085 | - |
gene disruption mutant, pH 7.4 | Plasmodium falciparum | immucillin-H | |
0.000016 | - |
gene disruption mutant, pH 7.4 | Plasmodium falciparum | 2'-deoxy-immucillin-H | |
0.000043 | - |
wild-type, pH 7.4 | Plasmodium falciparum | immucillin-H | |
0.000086 | - |
wild-type, pH 7.4 | Plasmodium falciparum | 5'-methylthio-immucillin-H | |
0.00044 | - |
wild-type, pH 7.4 | Plasmodium falciparum | 2'-deoxy-immucillin-H | |
0.00053 | - |
wild-type, pH 7.4 | Plasmodium falciparum | 2'-deoxy-immucillin-G | |
0.0009 | - |
gene disruption mutant, pH 7.4 | Plasmodium falciparum | 5'-methylthio-immucillin-H |
General Information | Comment | Organism |
---|---|---|
physiological function | targeted gene disruption results in loss of enzyme activity in lysate, with normal activity of adenosine deaminase. Mutant parasites show a greater requirement for exogenous purines and a severe growth defect at physiological concentrations of hypoxanthine. The mutant parasites are more sensitive to purine nucleoside phosphorylase inhibitors that bind human purine nucelotide phosphorylase tighter and less sensitive to inhibitor 5'-methylthio-immucillin-H | Plasmodium falciparum |