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Literature summary for 2.4.2.1 extracted from

  • Erion, M.D.; Takabayashi, K.; Smith, H.B.; Kessi, J.; Wagner, S.; Hoenger, S.; Shames, S.L.; Ealick, S.E.
    Purine nucleoside phosphorylase. 1. Structure-function Studies (1997), Biochemistry, 36, 11725-11734.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of site-directed mutants in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
H86A 10-25fold reduction in catalytic activity Homo sapiens
N243A 100fold decrease in turnover-number compared to wild-type enzyme Homo sapiens
Q89A 10-25fold reduction in catalytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
hypoxanthine mutant enzyme Y88F Homo sapiens
0.013
-
hypoxanthine mutant enzyme S33A Homo sapiens
0.019
-
hypoxanthine mutant enzyme H86A Homo sapiens
0.02
-
hypoxanthine mutant enzyme R84A and H257A Homo sapiens
0.022
-
Inosine mutant enzymes N243A and T242A Homo sapiens
0.024
-
hypoxanthine mutant enzyme T242A Homo sapiens
0.025
-
hypoxanthine mutant enzyme F159A Homo sapiens
0.03
-
hypoxanthine wild-type enzyme Homo sapiens
0.037
-
Inosine mutant enzyme K244A Homo sapiens
0.04
-
Inosine wild-type enzyme Homo sapiens
0.04
-
hypoxanthine mutant enzyme K244A Homo sapiens
0.058
-
Inosine mutant enzyme Y88F Homo sapiens
0.13
-
Inosine mutant enzyme R84A Homo sapiens
0.14
-
Inosine mutant enzyme S33A Homo sapiens
0.18
-
hypoxanthine mutant enzyme M219A Homo sapiens
0.21
-
Inosine mutant enzyme H257A Homo sapiens
0.22
-
Inosine mutant enzyme E89A Homo sapiens
0.22
-
phosphate reaction with inosine, mutant enzyme T242A Homo sapiens
0.28
-
Inosine mutant enzyme H86A Homo sapiens
0.31
-
Inosine mutant enzyme F159A Homo sapiens
0.57
-
hypoxanthine mutant enzyme F200A Homo sapiens
1
-
hypoxanthine mutant enzyme E201A Homo sapiens
2.1
-
phosphate mutant enzyme Y88F Homo sapiens
2.3
-
hypoxanthine mutant enzyme N243A Homo sapiens
2.5
-
phosphate mutant enzymes H257A and F159A Homo sapiens
2.7
-
phosphate reaction with inosine, mutant enzyme K244A Homo sapiens
3.5
-
phosphate reaction with inosine, mutant enzyme N243A Homo sapiens
3.6
-
phosphate reaction with inosine, mutant enzyme S33A Homo sapiens
4
-
phosphate reaction with inosine, wild-type enzyme Homo sapiens
4.3
-
phosphate mutant enzyme M219A Homo sapiens
4.5
-
phosphate reaction with inosine, mutant enzyme F200A Homo sapiens
4.5
-
phosphate reaction with inosine, mutant enzyme R84A Homo sapiens
6.1
-
phosphate reaction with inosine, mutant enzyme E201A Homo sapiens
8
-
Inosine mutant enzyme M219A Homo sapiens
8.4
-
Inosine mutant enzyme E201A Homo sapiens
9.2
-
phosphate reaction with inosine, mutant enzyme H86A Homo sapiens
19
-
Inosine mutant enzyme F200A Homo sapiens
91
-
phosphate reaction with inosine, mutant enzyme E89A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant mutant enzymes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + phosphate
-
Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
Inosine mutant enzyme N243A Homo sapiens
0.09
-
phosphate reaction with inosine, mutant enzyme N243A Homo sapiens
0.5
-
hypoxanthine mutant enzyme R84A Homo sapiens
0.97
-
hypoxanthine mutant enzyme H86A Homo sapiens
1.1
-
Inosine mutant enzyme S33A Homo sapiens
1.1
-
phosphate reaction with inosine, mutant enzyme K244A Homo sapiens
1.4
-
hypoxanthine mutant enzyme E201A Homo sapiens
1.5
-
phosphate reaction with inosine, mutant enzyme R84A Homo sapiens
1.8
-
phosphate reaction with inosine, mutant enzyme E201A Homo sapiens
2.1
-
Inosine mutant enzyme R84A Homo sapiens
2.1
-
hypoxanthine mutant enzyme S33A Homo sapiens
4.2
-
Inosine mutant enzyme E201A Homo sapiens
6.2
-
phosphate reaction with inosine, mutant enzyme F200A Homo sapiens
9
-
hypoxanthine mutant enzyme Y88F Homo sapiens
9.4
-
Inosine mutant enzyme F200A Homo sapiens
9.4
-
phosphate reaction with inosine, mutant enzyme H86A Homo sapiens
10
-
hypoxanthine mutant enzyme H257A Homo sapiens
12
-
phosphate reaction with inosine, mutant enzyme T242A Homo sapiens
13
-
hypoxanthine mutant enzyme N243A Homo sapiens
13
-
phosphate reaction with inosine, mutant enzyme H257A Homo sapiens
14
-
hypoxanthine mutant enzyme F200A Homo sapiens
14
-
Inosine mutant enzymes T242A and H86A Homo sapiens
16
-
Inosine mutant enzyme H257A Homo sapiens
24
-
hypoxanthine mutant enzyme F159A Homo sapiens
30
-
phosphate reaction with inosine, mutant enzyme F159A Homo sapiens
32
-
Inosine mutant enzyme F159A Homo sapiens
41
-
phosphate reaction with inosine, mutant enzyme M219A Homo sapiens
46
-
Inosine mutant enzyme E89A Homo sapiens
49
-
Inosine mutant enzyme K244A Homo sapiens
52
-
phosphate reaction with inosine, mutant enzyme K244A Homo sapiens
52
-
Inosine mutant enzyme M219A Homo sapiens
53
-
phosphate reaction with inosine, mutant enzyme E89A Homo sapiens
54
-
Inosine mutant enzyme Y88F Homo sapiens
56
-
phosphate wild-type enzyme Homo sapiens
56
-
Inosine wild-type enzyme Homo sapiens
56
-
phosphate reaction with inosine, mutant enzyme Y88F Homo sapiens
58
-
hypoxanthine mutant enzyme T242A Homo sapiens
98
-
hypoxanthine mutant enzyme K244A Homo sapiens
110
-
hypoxanthine wild-type enzyme Homo sapiens
130
-
hypoxanthine mutant enzyme M219A Homo sapiens