Protein Variants | Comment | Organism |
---|---|---|
M64V | mutant enzyme is able to cleave numerous 5-modified nucleoside analogs with much greater efficiency than the wild-type enzyme | Escherichia coli |
M64V | naturally occuring mutant, the mutant enzyme is able to cleave numerous 5-modified nucleoside analogs with much greater efficiency than the wild-type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-fluoro-2-deoxyadenosine + phosphate | Escherichia coli | a toxic prodrug | ? | - |
? | |
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate | Escherichia coli | a toxic prodrug | ? | - |
? | |
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate | Escherichia coli | a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity | ? | - |
? | |
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate | Escherichia coli | a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity | ? | - |
? | |
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate | Escherichia coli | a toxic prodrug | ? | - |
? | |
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate | Escherichia coli | a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
mutant enzyme M64V | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
wild-type enzyme, substrate 9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine | Escherichia coli |
0.0167 | - |
mutant M64V, substrate 9-[beta-D-arabinofuranosyl]-2-fluoro-adenine | Escherichia coli |
0.02 | - |
wild-type enzyme, substrate 9-[beta-D-arabinofuranosyl]-2-fluoro-adenine | Escherichia coli |
0.13 | - |
wild-type enzyme, substrate 9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine | Escherichia coli |
0.567 | - |
wild-type enzyme, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine | Escherichia coli |
1.43 | - |
mutant M64V, substrate 9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine | Escherichia coli |
4 | - |
mutant M64V, substrate 9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine | Escherichia coli |
7.3 | - |
wild-type enzyme, substrate 2-fluoro-2'-deoxyadenosine | Escherichia coli |
8.83 | - |
wild-type enzyme, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine | Escherichia coli |
9.83 | - |
mutant M64V, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine | Escherichia coli |
23.3 | - |
mutant M64V, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine | Escherichia coli |
26.67 | - |
mutant M64V, substrate 2-fluoro-2'-deoxyadenosine | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-fluoro-2-deoxyadenosine + phosphate | a toxic prodrug | Escherichia coli | ? | - |
? | |
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate | a toxic prodrug | Escherichia coli | ? | - |
? | |
9-[6-deoxy-alpha-L-talofuranosyl]-2-F-adenine + phosphate | - |
Escherichia coli | ? | - |
? | |
9-[6-deoxy-alpha-L-talofuranosyl]-6-methylpurine + phosphate | - |
Escherichia coli | ? | - |
? | |
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate | a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity | Escherichia coli | ? | - |
? | |
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate | a toxic prodrug, substrate of enzyme mutant M64V | Escherichia coli | ? | - |
? | |
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate | a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity | Escherichia coli | ? | - |
? | |
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate | a toxic prodrug, substrate of enzyme mutant M64V, liberation of methylpurine | Escherichia coli | ? | - |
? | |
9-[alpha-L-lyxofuranosyl]-2-F-adenine + phosphate | - |
Escherichia coli | ? | - |
? | |
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate | a toxic prodrug | Escherichia coli | ? | - |
? | |
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate | a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity | Escherichia coli | ? | - |
? | |
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate | a toxic prodrug, substrate of enzyme mutant M64V | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
purine nucleoside phosphorylase | - |
Escherichia coli |