Information on EC 2.7.11.11 - cAMP-dependent protein kinase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
2.7.11.11
-
RECOMMENDED NAME
GeneOntology No.
cAMP-dependent protein kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (cAMP-dependent)
cAMP is required to activate this enzyme. The inactive holoenzyme of cAMP-dependent protein kinase is a tetramer composed of two regulatory (R) and two catalytic (C) subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits [i.e. R2C2 + 4 cAMP = R2(cAMP)4 + 2 C].
CAS REGISTRY NUMBER
COMMENTARY hide
142008-29-5
-
142008-29-5
cAMP-dependent protein kinase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
male ticks, three isozymes
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
genes kin-1 and F47F2.1b encode two PK-A-like catalytic subunits
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
catfish, female
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Manually annotated by BRENDA team
strain JC100
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Manually annotated by BRENDA team
strain JC100
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Mus musculus BALB/c
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Manually annotated by BRENDA team
Mus musculus C57BL/6
C57/BL6 mice
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Manually annotated by BRENDA team
FVB/N mice
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
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Manually annotated by BRENDA team
strain Y258
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Manually annotated by BRENDA team
sea urchin
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5HT7 receptor
ADP + phosphorylated 5HT7 receptor
show the reaction diagram
-
recombinant rat 5HT7 receptor reconstituted in frog oocytes, overview
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + A-kinase anchor protein
ADP + phosphorylated A-kinase anchor protein
show the reaction diagram
ATP + actin
ADP + phosphorylated actin
show the reaction diagram
ATP + adenylate kinase 1
ADP + phosphorylated adenylate kinase 1
show the reaction diagram
ATP + adenylate kinase 5
ADP + phosphorylated adenylate kinase 5
show the reaction diagram
ATP + aquaporin
ADP + phosphorylated aquaporin
show the reaction diagram
-
-
-
-
?
ATP + ATPase
ADP + phosphorylated ATPase
show the reaction diagram
-
-
-
-
?
ATP + autophagy-related protein kinase Atg1
ADP + phosphorylated autophagy-related protein kinase Atg1
show the reaction diagram
ATP + beta-adrenergic receptor
ADP + phosphorylated beta-adrenergic receptor
show the reaction diagram
ATP + beta-catenin
ADP + phosphorylated beta-cateniin
show the reaction diagram
ATP + beta1-adrenergic receptor
ADP + phosphorylated beta1-adrenergic receptor
show the reaction diagram
ATP + BKCa channel ZERO
ADP + phosphorylated BKCa channel ZERO
show the reaction diagram
ATP + cAMP response element binding protein
ADP + cAMP response element binding protein
show the reaction diagram
-
-
-
-
?
ATP + cAMP response element binding protein
ADP + phosphorylated cAMP response element binding protein
show the reaction diagram
-
the transcription factor cAMP response element binding protein is directly activated by PKA
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-
?
ATP + cAMP-response element-binding protein
ADP + phosphorylated cAMP-response element-binding protein
show the reaction diagram
-
-
-
-
?
ATP + cAMP-responsive element binding protein
ADP + phosphorylated cAMP-responsive element binding protein
show the reaction diagram
ATP + cAMP-responsive element-binding protein
ADP + phosphorylated cAMP-responsive element-binding protein
show the reaction diagram
-
-
-
-
?
ATP + carboxyl-terminal binding protein 1
ADP + phosphorylated carboxyl-terminal binding protein 1
show the reaction diagram
-
-
-
-
?
ATP + casein
ADP + phosphorylated casein
show the reaction diagram
ATP + catchin
ADP + phosphorylated catchin
show the reaction diagram
-
low activity
-
-
?
ATP + Cav1.2
ADP + phosphorylated Cav1.2
show the reaction diagram
ATP + Cav3.2 T-type Ca2+ channel
ADP + phosphorylated Cav3.2 T-type Ca2+ channel
show the reaction diagram
-
recombinant human Ca2+ channel reconstituted in frog oocytes, overview
-
-
?
ATP + cellular nucleic acid binding protein
ADP + phosphorylated cellular nucleic acid binding protein
show the reaction diagram
ATP + ChChd3
ADP + phosphorylated ChChd3
show the reaction diagram
-
an endogenous mitochondrial protein
-
-
?
ATP + class III phosphatidylinositol 3-phosphate kinase
ADP + phosphorylated class III phosphatidylinositol 3-phosphate kinase
show the reaction diagram
-
-
-
-
?
ATP + claudin-3
ADP + phosphorylated claudin-3
show the reaction diagram
ATP + claudin-3 S199A
ADP + phosphorylated claudin-3 S199A
show the reaction diagram
-
recombinant GST-fusion claudin-3 mutant S199A expressed in Escherichia coli, phosphorylation at Thr192, activity of catalytic PKA subunit is lower compared to wild-type claudin-3
-
-
?
ATP + CRE-binding protein
ADP + CRE-binding phosphoprotein
show the reaction diagram
-
i.e. CREB, a transcription factor, activition by type I PKA
-
-
?
ATP + creatine kinase
ADP + phosphorylated creatine kinase
show the reaction diagram
ATP + CREB protein
ADP + CREB phosphoprotein
show the reaction diagram
ATP + CREB-binding protein
ADP + CREB-binding phosphoprotein
show the reaction diagram
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i.e. CBP, activition by type II PKA
-
-
?
ATP + DNA excision repair protein
ADP + phosphorylated DNA excision repair protein
show the reaction diagram
-
-
-
-
?
ATP + Dot6
ADP + phosphorylated Dot6
show the reaction diagram
ATP + EPS8 protein
ADP + EPS8 phosphoprotein
show the reaction diagram
ATP + extracellular signal-regulated kinase
ADP + phosphorylated extracellular signal-regulated kinase
show the reaction diagram
-
-
-
-
?
ATP + ezrin
ADP + phosphoezrin
show the reaction diagram
ATP + fat body triglyceride lipase
ADP + phosphorylated fat body triglyceride lipase
show the reaction diagram
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protein substrate is phosphorylated at Ser563, but not activated by the enzyme, Ser563 is the regulatory site of the enzyme substrate
-
-
?
ATP + G protein-coupled receptor GRK1
ADP + phosphorylated G protein-coupled receptor GRK1
show the reaction diagram
ATP + G protein-coupled receptor GRK7
ADP + phosphorylated G protein-coupled receptor GRK7
show the reaction diagram
ATP + glycogen synthase kinase 3beta
ADP + phosphorylated glycogen synthase kinase 3beta
show the reaction diagram
-
phosphorylation of glycogen synthase kinase 3beta is carried out by the PKA type II isoform
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-
?
ATP + hemagglutin-tagged PKI peptide
ADP + phosphorylated hemagglutin-tagged PKI peptide
show the reaction diagram
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-
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?
ATP + hexokinase
ADP + phosphorylated hexokinase
show the reaction diagram
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-
-
-
?
ATP + histone
ADP + phosphorylated histone
show the reaction diagram
ATP + histone 3s
ADP + phosphorylated histone 3s
show the reaction diagram
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activity of the catalytic subunit Cgamma
-
-
?
ATP + histone deacetylase 8
ADP + phosphorylated histone deacetylase 8
show the reaction diagram
ATP + histone H2A
ADP + phosphorylated histone H2A
show the reaction diagram
ATP + histone H3S
ADP + phosphorylated histone H3S
show the reaction diagram
ATP + histone IIIS
ADP + phosphorylated histone IIIS
show the reaction diagram
-
activity of the catalytic subunit Cgamma
-
-
?
ATP + inositol 1,4,5-trisphosphate receptor-I
ADP + phosphorylated inositol 1,4,5-trisphosphate receptor-I
show the reaction diagram
-
isolated catalytic subunit of PKA, selective phosphorylation at Ser1589 and Ser1755
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-
?
ATP + IP3R-2
ADP + phosphorylated IP3R-2
show the reaction diagram
ATP + Kemptide
ADP + Kemptide phosphate
show the reaction diagram
ATP + kemptide
ADP + phospho-kemptide
show the reaction diagram
ATP + Kemptide
ADP + phosphorylated Kemptide
show the reaction diagram
ATP + L-pyruvate kinase
ADP + phosphorylated L-pyruvate kinase
show the reaction diagram
-
recombinant rat protein substrate expressed in Escherichia coli, phosphorylation at Ser12, activity of catalytic PKA subunit
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-
?
ATP + lactate dehydrogenase subunit A mRNA
ADP + 3'-UTR phosphorylated lactate dehydrogenase subunit A mRNA
show the reaction diagram
ATP + Leu-Arg-Arg-Ala-Ser-Leu-Gly
ADP + Leu-Arg-Arg-Ala-phospho-Ser-Leu-Gly
show the reaction diagram
ATP + low-density lipoprotein-related protein
ADP + phosphorylated low-density lipoprotein-related protein
show the reaction diagram
-
-
-
-
?
ATP + LRRASLG
ADP + LRRA-phosphoserine-LG
show the reaction diagram
ATP + maltose binding protein
ADP + phosphorylated maltose binding protein
show the reaction diagram
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enzyme activity is preferably activated by cAMP, 35% lower activity with cGMP
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?
ATP + merlin
ADP + phosphorylated merlin
show the reaction diagram
ATP + mitogen-activated protein kinase phosphatase-1
ADP + phosphorylated mitogen-activated protein kinase phosphatase-1
show the reaction diagram
ATP + myocyte enhancer factor 2D
ADP + phosphorylated myocyte enhancer factor 2D
show the reaction diagram
-
myocyte enhancer factor 2D serine 121 and serine 190 are targeted by PKA
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?
ATP + N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFSYP-amide
ADP + N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFpSYP-amide
show the reaction diagram
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-
-
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?
ATP + N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFSYP-amide
ADP + N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFpSYP-amide
show the reaction diagram
-
-
-
-
?
ATP + N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFSYP-amide
ADP + N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFpSYP-amide
show the reaction diagram
-
-
-
-
?
ATP + Nav1.4 T-type Na+ channel
ADP + phosphorylated Nav1.4 T-type Na+ channel
show the reaction diagram
-
recombinant rat Na+ channel reconstituted in frog oocytes, overview
-
-
?
ATP + NDUFS-4 protein
ADP + phosphorylated NDUFS-4 protein
show the reaction diagram
-
-
-
-
?
ATP + NDUFS4 subunit of complex I
ADP + phosphorylated NDUFS4 subunit of complex I
show the reaction diagram
ATP + NF-kappaB p65
ADP + phoshorylated NF-kappaB p65
show the reaction diagram
-
phosphorylation by the catalytic subunit of PKA
-
-
?
ATP + p270 protein
ADP + phosphorylated p270 protein
show the reaction diagram
-
-
-
-
?
ATP + paramyosin
ADP + phosphorylated paramyosin
show the reaction diagram
-
low activity
-
-
?
ATP + PDE11A protein
ADP + PDE11A phosphoprotein
show the reaction diagram
ATP + PDE5A protein
ADP + PDE5A phosphoprotein
show the reaction diagram
ATP + phosphatase inhibitor PKI
ADP + phosphorylated phosphatase inhibitor PKI
show the reaction diagram
-
-
-
?
ATP + phosphatidylinositol 3-kinase
ADP + phosphorylated phosphatidylinositol 3-kinase
show the reaction diagram
-
-
-
-
?
ATP + phosphodiesterase-4
ADP + phosphorylated phosphodiesterase-4
show the reaction diagram
-
-
-
-
?
ATP + phosvitin
ADP + phosphorylated phosvitin
show the reaction diagram
-
enzyme activity is preferably activated by cAMP, 33% lower activity with cGMP
-
-
?
ATP + PKA regulatory subunit IIalpha
ADP + phosphorylated PKA regulatory subunit IIalpha
show the reaction diagram
-
-
-
-
?
ATP + PLARTLSVAGLPGKK
ADP + PLARTL-phosphoserine-VAGLPGKK
show the reaction diagram
-
syntide 2-derived peptide substrate
-
-
?
ATP + protamine sulfate
ADP + phosphoprotamine sulfate
show the reaction diagram
-
enzyme activity is about equally activated by cAMP and cGMP, and derivatives of cAMP
-
-
?
ATP + protein tyrosine phosphatase
ADP + phosphorylated protein tyrosine phosphatase
show the reaction diagram
ATP + pyruvate kinase
ADP + phosphorylated pyruvate kinase
show the reaction diagram
-
enzyme activity is about equally activated by cAMP and cGMP
-
-
?
ATP + RFARKGSLREKNV
ADP + RFARKG-phosphoserine-LREKNV
show the reaction diagram
ATP + RFARKGSLRQKNV
ADP + RFARKG-phosphoserine-LRQKNV
show the reaction diagram
-
PKC-derived peptide substrate
-
-
?
ATP + RGS protein
ADP + RGS protein phosphate
show the reaction diagram
ATP + ribosomal protein S6
ADP + phosphorylated ribosomal protein S6
show the reaction diagram
ATP + ribosomal S6 protein
ADP + phosphorylated ribosomal S6 protein
show the reaction diagram
-
-
-
-
?
ATP + RKRSRAE
ADP + RKR-phosphoserine-RAE
show the reaction diagram
ATP + RKRSRKE
ADP + RKR-phosphoserine-RKE
show the reaction diagram
ATP + RRASVA
ADP + RRA-phosphoserine-VA
show the reaction diagram
-
pyruvate kinase-derived peptide substrate comprising the phosphorylation site around Ser12 of the protein, activity of catalytic PKA subunit
-
-
?
ATP + RRLSSLRA
ADP + RRL-phosphoserine-phosphoserine-LRA
show the reaction diagram
ATP + ryanodine receptor
ADP + phosphorylated ryanodine receptor
show the reaction diagram
-
-
-
-
?
ATP + small heat shock protein HSP22
ADP + phosphorylated small heat shock protein HSP22
show the reaction diagram
-
-
-
-
?
ATP + small heat shock-related protein HSP20
ADP + phosphorylated small heat shock-related protein HSP20
show the reaction diagram
-
-
-
-
?
ATP + SP20
ADP + SP20 phosphate
show the reaction diagram
-
substrate peptide, the C subunit SP20-binding residues are E203, F129, E170, E230, D166, and K168
-
-
?
ATP + SP20 peptide
ADP + phosphorylated SP20 peptide
show the reaction diagram
-
-
-
-
?
ATP + syntaphilin
ADP + syntaphilin phosphate
show the reaction diagram
-
-
-
-
?
ATP + tomosyn
ADP + tomosyn phosphate
show the reaction diagram
-
-
-
-
?
ATP + twitchin
ADP + phosphorylated twitchin
show the reaction diagram
ATP + type III inositol 1,4,5-trisphosphate receptor
ADP + phosphorylated type III inositol 1,4,5-trisphosphate receptor
show the reaction diagram
ATP + tyrosine hydroxylase
ADP + phosphorylated tyrosine hydroxylase
show the reaction diagram
ATP + ubiquinol-cytochrome c reductase complex core protein 2
ADP + ubiquinol-cytochrome c reductase complex core phosphoprotein 2
show the reaction diagram
ATP + VASP
ADP + phosphorylated VASP
show the reaction diagram
ATP + [cystic fibrosis transmembrane conductance regulator protein]
ADP + [cystic fibrosis transmembrane conductance regulator phosphoprotein]
show the reaction diagram
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
ATP + [Whi3 cell cycle regulator protein]
ADP + [Whi3 cell cycle regulator phosphoprotein]
show the reaction diagram
N6-benzyl-ATP + ChChd3
N6-benzyl-ADP + phosphorylated ChChd3
show the reaction diagram
-
cofactor of mutant M120G, poor activity with the wild-type catalytic subunit
-
-
?
N6-phenethyl-ATP + ChChd3
N6-phenethyl-ADP + phosphorylated ChChd3
show the reaction diagram
-
cofactor of mutant M120G, poor activity with the wild-type catalytic subunit
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + A-kinase anchor protein
ADP + phosphorylated A-kinase anchor protein
show the reaction diagram
-
phosphorylation at Ser1928
-
-
?
ATP + actin
ADP + phosphorylated actin
show the reaction diagram
ATP + adenylate kinase 1
ADP + phosphorylated adenylate kinase 1
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + adenylate kinase 5
ADP + phosphorylated adenylate kinase 5
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + autophagy-related protein kinase Atg1
ADP + phosphorylated autophagy-related protein kinase Atg1
show the reaction diagram
ATP + beta-adrenergic receptor
ADP + phosphorylated beta-adrenergic receptor
show the reaction diagram
-
PKA phosphorylation mediates beta-1 adrenergic receptor endocytosis via the caveolae pathway
-
-
?
ATP + beta-catenin
ADP + phosphorylated beta-cateniin
show the reaction diagram
-
-
-
-
?
ATP + beta1-adrenergic receptor
ADP + phosphorylated beta1-adrenergic receptor
show the reaction diagram
-
recombinant beta1-adrenergic receptor expressed in HEK-293 cell membranes, phosphorylation at Ser312 is essential for activation of endocytic recycling of the agonist-internalized beta1-adrenergic receptor, beta1-AR mutant S312A is not recycled, overview
-
-
?
ATP + BKCa channel ZERO
ADP + phosphorylated BKCa channel ZERO
show the reaction diagram
-
recombinant murine HA-tagged tetrameric protein expressed in HEK-293 cells, activity with wild-type and mutant Y334V channel tetramers, but no activity with S899A mutant, phosphorylation at Ser899 activates the channels, overview
-
-
?
ATP + cAMP-responsive element binding protein
ADP + phosphorylated cAMP-responsive element binding protein
show the reaction diagram
ATP + catchin
ADP + phosphorylated catchin
show the reaction diagram
-
low activity
-
-
?
ATP + Cav1.2
ADP + phosphorylated Cav1.2
show the reaction diagram
-
in anchoring to the L-type calcium channel Cav1.2 via A-kinase anchor protein 150 in neurons plays a critical role involving phosphorylation by the enzyme, PKA increases the activity of the L-type Ca2+ channel Cav1.2 in response to beta-adrenergic stimulation in heart and brain
-
-
?
ATP + cellular nucleic acid binding protein
ADP + phosphorylated cellular nucleic acid binding protein
show the reaction diagram
-
CNBP performs a fine tune expression regulation of a group of target including c-myc, during vertebrate embryogenesis, different phosphorylation patterns at different development al stages, overview
-
-
?
ATP + claudin-3
ADP + phosphorylated claudin-3
show the reaction diagram
-
high activity in ovarian cancer cells with recombinantly overexpressed claudin-3, phosphorylation of claudin-3 affecting the barrier function with extracellular Ca2+, overview
-
-
?
ATP + CRE-binding protein
ADP + CRE-binding phosphoprotein
show the reaction diagram
-
i.e. CREB, a transcription factor, activition by type I PKA
-
-
?
ATP + creatine kinase
ADP + phosphorylated creatine kinase
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + CREB protein
ADP + CREB phosphoprotein
show the reaction diagram
ATP + CREB-binding protein
ADP + CREB-binding phosphoprotein
show the reaction diagram
-
i.e. CBP, activition by type II PKA
-
-
?
ATP + Dot6
ADP + phosphorylated Dot6
show the reaction diagram
-
a protein implicated in telomere function
-
-
?
ATP + EPS8 protein
ADP + EPS8 phosphoprotein
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + ezrin
ADP + phosphoezrin
show the reaction diagram
-
recombinant GST-tagged wild-type ezrin transiently expressed in primary gastric parietal cells from stomach, recombinant mutant S66A and S66D ezrin proteins are no substrates, phosphorylation of ezrin leads to activation of parietal cells required for dilation of apical vacuolar membrane and histamine-stimulated acid secretion in gastric mucosa, overview
-
-
?
ATP + G protein-coupled receptor GRK1
ADP + phosphorylated G protein-coupled receptor GRK1
show the reaction diagram
ATP + G protein-coupled receptor GRK7
ADP + phosphorylated G protein-coupled receptor GRK7
show the reaction diagram
ATP + histone deacetylase 8
ADP + phosphorylated histone deacetylase 8
show the reaction diagram
-
FLAG-tagged class 1 histone deacetylase HDAC8 expressed in HeLa cells via adenovirus infection, phosphorylation at Ser39 reduces the enzyme activity of HDAC8, hyperphosphorylation inhibits the enzyme
-
-
?
ATP + IP3R-2
ADP + phosphorylated IP3R-2
show the reaction diagram
-
phosphoregulation of the inositol 1,4,5-trisphosphate receptor subtype 2, PKA enhances inositol 1,4,5-trisphosphate-induced Ca2+ release in AR4-2J cells, regulation, overview
-
-
?
ATP + lactate dehydrogenase subunit A mRNA
ADP + 3'-UTR phosphorylated lactate dehydrogenase subunit A mRNA
show the reaction diagram
-
the enzyme stabilizes lactate dehydrogenase LDH-A mRNA and increases intracellular LDH-A mRNA levels by phosphorylation of a cAMP-stabilizing region CSR on the 3'-untranslated region of the LDH-A mRNA, regulation, mechanism
-
-
?
ATP + merlin
ADP + phosphorylated merlin
show the reaction diagram
-
recombinant human substrate protein expressed in HEK-293 cells, activity of the catalytic subunit C, phosphorylation at Ser518 induces N-terminal binding of merlin to ezrin
-
-
?
ATP + mitogen-activated protein kinase phosphatase-1
ADP + phosphorylated mitogen-activated protein kinase phosphatase-1
show the reaction diagram
-
the enzyme enhances steroid hydroxylase CYP17 transcription via mitogen-activated protein kinase phosphatase-1 MKP-1 activation in H295R adrenocortical cells
-
-
?
ATP + NDUFS4 subunit of complex I
ADP + phosphorylated NDUFS4 subunit of complex I
show the reaction diagram
ATP + paramyosin
ADP + phosphorylated paramyosin
show the reaction diagram
-
low activity
-
-
?
ATP + PDE11A protein
ADP + PDE11A phosphoprotein
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + PDE5A protein
ADP + PDE5A phosphoprotein
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + RGS protein
ADP + RGS protein phosphate
show the reaction diagram
-
recombinant HA-tagged substrate expressed in B35 cells, phosphorylation of RGS14 by PKA potentiates its activity toward Galphai-GDP
-
-
?
ATP + ribosomal S6 protein
ADP + phosphorylated ribosomal S6 protein
show the reaction diagram
-
-
-
-
?
ATP + twitchin
ADP + phosphorylated twitchin
show the reaction diagram
-
-
-
-
?
ATP + type III inositol 1,4,5-trisphosphate receptor
ADP + phosphorylated type III inositol 1,4,5-trisphosphate receptor
show the reaction diagram
-
i.e. IP3 receptor, recombinantly expressed in HEK cells, forms tetrameric Ca2+ channels in the endoplasmic reticulum
-
-
?
ATP + tyrosine hydroxylase
ADP + phosphorylated tyrosine hydroxylase
show the reaction diagram
-
enzyme is involved in the signal transduction regulatory mechanism in the triiodothyronine T3-activation of forebrain tyrosine hydroxylase
-
-
?
ATP + ubiquinol-cytochrome c reductase complex core protein 2
ADP + ubiquinol-cytochrome c reductase complex core phosphoprotein 2
show the reaction diagram
-
sperm protein substrate
-
-
?
ATP + [cystic fibrosis transmembrane conductance regulator protein]
ADP + [cystic fibrosis transmembrane conductance regulator phosphoprotein]
show the reaction diagram
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
ATP + [Whi3 cell cycle regulator protein]
ADP + [Whi3 cell cycle regulator phosphoprotein]
show the reaction diagram
-
phosphorylation of Ser568 leads to inhibition of Whi3
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N6-benzyl-ATP
-
preferred co-substrate of catalytic subunit mutant M120G, but inhibitory versus kemptide, overview
N6-phenethyl-ATP
-
preferred co-substrate of catalytic subunit mutant M120G, but inhibitory versus kemptide, overview
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
extracellular Ca2+ is required for enzyme activity in sperm
Cd2+
-
can partially substitue Mg2+
Co2+
-
can partially substitue Mg2+
Mn2+
-
can partially substitue Mg2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(Rp)-adenosine 3',5'-cyclic monophosphorothionate triethyl ammonium salt
-
inhibitor of PKA
(Rp)-adenosine 3',5'-cyclic monophosphothioate
-
i.e. (Rp)-cAMPS, analogue of cAMP, competitive inhibition of PKA activation by cAMP, binding site structure
(Rp)-adenosine-3',5'-cyclic monophosphorothioate
(Sp)-adenosine 3',5'-cyclic monophosphothioate
-
i.e. (Sp)-cAMPS, analogue of cAMP, competitive inhibition of PKA activation by cAMP, binding site structure
7-hydroxystaurosporine
-
specific protein kinase inhibitor
Acetylsalicylic acid
AdcAhxArg6
-
i.e. adenosine 5'-carboxylic acid-6-aminohexanoic acid-L-arginine, peptide-nucleoside conjugate inhibitor, inhibition of the catalytic subunit, binding mechanism
adenosine 3',5'-cyclic phosphorothioate
-
-
adenosine 5'-(beta,gamma-Imino)triphosphate
-
binding structure at the ATP binding site
ADP
-
noncompetitive inhibition with respect to ATP
alsterpaullone
-
21% inhibition of CDK2 at 0.01 mM
bicarbonate
-
p270 phosphorylation is not observed when sperms are incubated with M2 medium that contains 4.2 mM bicarbonate
Ca2+
-
strong inhibition
ethylmaleimide
-
complete inhibition at 2 mM
genistein
-
partial inhibition
guanethidine
-
noncompetitive inhibiting serine peptide analogue with respect to ATP
H-85
-
unspecific kinase inhibitor, inhibits 23% of the acrosome reaction in sperm at 0.03 mM
HA1077
-
partial inhibition
imatinib
competes for the ATP binding site; competes for the ATP binding site
indirubin-3'-monoxime
-
20% inhibition of CDK2 at 0.01 mM
inhibitor H89
-
ATP competitive chemical inhibitor H89
inhibitor peptide PKI5-24
-
inhibits the catalytic subunit
-
KT-5720
KT5720
Mn2+
-
strong inhibition
myristoylated PKA peptide inhibitor
-
14-22 amide, blocks egg jelly layer induction of PKA
-
myristoylated PKI
-
specific inhibitor
-
myristoylated PKI14-22 amide
-
selective peptide inhibitor of P KA
-
myristoylated protein kinase A inhibitor
-
0.01 mM, specific inhibitor
-
myristoylated protein kinase I (14-22) amide
-
specific PKA inhibitor
-
myristoylated-PKI
-
cAMP-PKA-specific inhibitory peptide PKI
-
N-[2-(4-bromocinnamylamino) ethyl]-5-isoquinolinesulfonamide dihydrochloride
-
-
N6-benzyl-ATP
-
preferred co-substrate of catalytic subunit mutant M120G, but inhibitory for the mutant, not the wild-type enzyme, versus kemptide, overview
N6-phenethyl-ATP
-
preferred co-substrate of catalytic subunit mutant M120G, but inhibitory for the mutant, not the wild-type enzyme, versus kemptide, overview
naproxen
Ni2+
-
inhibits sperm protein phosphorylation by the enzyme
nifedipine
-
inhibits sperm protein phosphorylation by the enzyme
nimesulide
peptide inhibitor PKI
-
-
-
piroxicam
PKA regulatory subunit Ialpha
-
pseudosubstrate inhibitor
-
protein kinase A inhibitor peptide PKI
-
-
-
protein kinase A inhibitor PKI peptide
-
significant inhibition at 0.01 mM
-
protein kinase inhibitor
protein kinase inhibitor peptide
-
PKI
-
protein kinase inhibitor PKI
-
binding structure, overview
-
pseudosubstrate peptides
purvalanol
-
18% inhibition of CDK2 at 0.01 mM
regulatory subunit 1alpha
-
-
-
RIIalpha subunit
-
-
-
roscovitine
-
7% inhibition of CDK2 at 0.01 mM
Rp-8-CPT-cAMPS
-
-
Rp-cAMP
-
0.5 mM significantly reduces forskolin- and dibutyryl-cAMP-induced PKA activation
staurosporine
stearate peptide st-Ht31
-
-
-
STVHEILCKLSLEG
-
an acetylated peptide Ac1-14, but not the nonacetylated equivalent N1-14, inhibits PKA-dependent outwardly rectifying Cl- channels, ORCC, and CFTRmediated currents, the peptide sequence is equivalent to the S100A10 binding site on anx 2, it disrupts the anx S100A10/CTFR interaction
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-isobutyryl-1-methylxanthine
-
stimulates activity at 0.1 mM
6-benzyl-cAMP
activator of PfPKA catalytic subunit; activator of PfPKA catalytic subunit
-
8-bromo-cAMP
8-chloro-cAMP
-
activates to same extent as cAMP
8-CPT-cAMP
-
induces release of the active catalytic subunits from the PKA holoenzyme
8-p-chloro-phenylthio-cAMP
-
0.1 mM activates
adipokinetic hormone
-
rapid enzyme activation
Adrenocorticotropin
-
induces PKA activation
-
Calcineurin
-
i.e. CaN, is important for the cAMP/PKA-dependent anx 2S100A10 complex formation
-
calpain
-
activates the PKA holoenzyme in a cAMP-independent fashion. A variety of agents, including inhibitors of the electron transport chain, activate calpain in a Ca2+-dependent manner, which, in turn, catalyzes the proteolysis of the R subunit, thereby releasing the C subunit in its active form, overview
-
cAMP-response element-binding protein-binding protein/p300
-
PKA coactivator
-
D-glucose
-
increases phosphorylation of beta-catenin on Ser552 in INS-1E beta-cells
dibutyryl-cAMP
forskolin
IBMX
-
0.5 mM activates
isoproterenol
Lactic acid
-
increases intracellular cAMP, pCREB and PKA activity
N6,2'-O-dibutyryl-cAMP
-
increases phosphorylation of beta-catenin on Ser552 in INS-1E beta-cells
N6,2'-O-dibutyryladenosine 3',5'-cyclic phosphate
-
induces PKA activation
N6-benzoyl-cAMP
-
0.1 mM activates
N6-benzoyladenosine-3',5'-cyclic monophosphate
-
PKA-specific cAMP analog
N6-monobutyryl-3'-5'-cAMP
-
specific protein kinase A activator, direct stimulation of acivity with 1 mM N6-monobutyryl-3'-5'-cAMP is necessary for the alkaline pH-induced accumulation of vacuolar H+-ATPase in clear cell apical microvilli
N6-Monobutyryl-cAMP
-
0.1 mM activates
prostaglandin E1
-
activates
prostaglandin E2
-
0.05 mM induce activity by elevation of cAMP levels
Ras-2 protein
-
-
-
Sp-adenosine 3',5'-cyclic monophosphorothioate triethylammonium salt hydrate
-
activates PKA
Sucrose
-
direct inducer of cAMP-PKA signaling
transforming growth factor beta
-
more than 2fold increased activity after 15 min at 0.1 nM
-
Vasoactive intestinal peptide
-
i.e. VIP
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0174 - 0.032
ATP
0.73
histone
-
pH 7.0, 22C, catalytic subunit
0.0125 - 0.038
Kemptide
0.023 - 0.043
Leu-Arg-Arg-Ala-Ser-Leu-Gly
0.039
MgATP2-
-
pH 7.0, 22C, catalytic subunit