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Literature summary for 2.7.11.11 extracted from

  • Zawadzki, K.M.; Taylor, S.S.
    cAMP-dependent protein kinase regulatory subunit type IIbeta: active site mutations define an isoform-specific network for allosteric signaling by cAMP (2004), J. Biol. Chem., 279, 7029-7036.
    View publication on PubMed
Search for more literature for 2.7.11.11

Activating Compound

Activating Compound Comment Organism Structure
cAMP each monomer of the tetrameric enzyme contains 2 bindig sites A and B for cAMP, binding involves Arg230 of domain A and Arg359 in domain B of regulatory subunit RIIbeta, cAMP binding releases the catalytic subunits Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of a deletion mutant of regulatory subunit RIIbeta lacking the complete domain B Rattus norvegicus
R230K site-directed mutagenesis, mutation of an A domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding Rattus norvegicus
R359K site-directed mutagenesis, mutation of a B domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics for cAMP binding to wild-type and mutant regulatory subunits RIIbeta, overview Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta, by nickel affinity chromatography and dialysis Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
reversible unfolding and folding in 8.5 M urea of recombinant His-tagged wild-type and mutant regulatory subunits RIIbeta, overview Rattus norvegicus

Subunits

Subunits Comment Organism
More holoenzyme structure Rattus norvegicus
tetramer 2 regulatory subunits in a dimer bound to 2 catalytic subunits, the latter are released during activation by cAMP Rattus norvegicus

Synonyms

Synonyms Comment Organism
cAPK
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus