Literature summary for 2.7.11.11 extracted from

Wu, J.; Jones, J.M.; Nguyen-Huu, X.; Ten Eyck, L.F.; Taylor, S.S.
Crystal structures of RIalpha subunit of cyclic adenosine 5'-monophosphate (cAMP)-dependent protein kinase complexed with (Rp)-adenosine 3',5'-cyclic monophosphothioate and (Sp)-adenosine 3',5'-cyclic monophosphothioate, the phosphothioate analogues of cAMP (2004), Biochemistry, 43, 6620-6629.

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Activating Compound

Activating Compound	Comment	Organism	Structure
cAMP	dependent on, binding site	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of regulatory subunit deletion mutant RIalpha comprising residues 1-91	Bos taurus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant regulatory subunit deletion mutant RIalpha bound to cAMP analogue ligands (Rp)-cAMPS or (Sp)-cAMPS resulting in (Rp)-RIalpha and (Sp)-RIalpha, hanging drop vapour diffusion method, mixing of 0.002ml protein solution containing 12 mg/ml protein in 50 mM MES, pH 7.5, 0.2 M NaCl, 2 mM EDTA, 2 mM EGTA, and 10 mM DTT, with 0.002 ml reservoir solution containing 1.0 M ammonium sulfate, 12.5% glycerol, 10 mM DTT, 0.1 M sodium acetate, pH 5.5, 22.5°C, 1 week, X-ray diffraction structure determination and analysis at 2.3-2.4 A resolution	Bos taurus

Crystallization (Comment)

Organism

purified recombinant regulatory subunit deletion mutant RIalpha bound to cAMP analogue ligands (Rp)-cAMPS or (Sp)-cAMPS resulting in (Rp)-RIalpha and (Sp)-RIalpha, hanging drop vapour diffusion method, mixing of 0.002ml protein solution containing 12 mg/ml protein in 50 mM MES, pH 7.5, 0.2 M NaCl, 2 mM EDTA, 2 mM EGTA, and 10 mM DTT, with 0.002 ml reservoir solution containing 1.0 M ammonium sulfate, 12.5% glycerol, 10 mM DTT, 0.1 M sodium acetate, pH 5.5, 22.5°C, 1 week, X-ray diffraction structure determination and analysis at 2.3-2.4 A resolution

Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
(Rp)-adenosine 3',5'-cyclic monophosphothioate	i.e. (Rp)-cAMPS, analogue of cAMP, competitive inhibition of PKA activation by cAMP, binding site structure	Bos taurus
(Sp)-adenosine 3',5'-cyclic monophosphothioate	i.e. (Sp)-cAMPS, analogue of cAMP, competitive inhibition of PKA activation by cAMP, binding site structure	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant regulatory subunit deletion mutant RIalpha by affinity chromatography, elution with cGMP, and dialysis	Bos taurus

Subunits

Subunits	Comment	Organism
More	structure of residues 1-91 of the regulatory subunit	Bos taurus

Synonyms

Synonyms	Comment	Organism
PKA	-	Bos taurus