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Literature summary for 2.7.11.11 extracted from
- Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP (2004), FEBS Lett., 576, 216-220.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP | dependent on, acts synergistically with cGMP, binding site domain structure on the regulatory subunit RII | Apis mellifera |  |
| cGMP | activates, acts synergistically with cAMP | Apis mellifera | |
| additional information | phosphate-binding cassettes PCB of domain A and B of the regulatory subunits | Apis mellifera |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| regulatory subunit RII, DNA sequence determination and analysis, phylogenetic analysis | Apis mellifera |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Apis mellifera | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apis mellifera | Q6ZXJ1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PKAII holoenzyme from bee brain by ion exchange, adsorption, and cAMP-affinity chromatography, thereby dissociation of regulatory and catalytic subunits, and reconstitution | Apis mellifera |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of holoenzyme from purified subunits in 50 mM Tris-HCl, pH 7.8, 1 mM EDTA, 1 mM EGTA, 10 mM 2-mercaptoethanol, and 0.1 M NaCl | Apis mellifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Apis mellifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phosphatase inhibitor PKI | - |
Apis mellifera | ADP + phosphorylated phosphatase inhibitor PKI | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure, deduced from DNA and amino acid sequence, analysis and comparison | Apis mellifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PKAII | - |
Apis mellifera |
| protein kinase A type II | - |
Apis mellifera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Apis mellifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Apis mellifera |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Apis mellifera | |
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Release 2023.1 (January 2023)
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