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Literature summary for 2.7.11.11 extracted from

  • Johnson, L.N.; Noble, M.E.M.; Owen, D.J.
    Active and inactive protein kinases: structural basis for regulation (1996), Cell, 85, 149-158.
    View publication on PubMed
Search for more literature for 2.7.11.11

Activating Compound

Activating Compound Comment Organism Structure
cAMP required for activity, activation is mediated by binding of cAMP to the regulatory subunits, causing release of the catalytic subunits Mammalia

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization in active conformation Mammalia

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-(beta,gamma-Imino)triphosphate binding structure at the ATP binding site Mammalia

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm primarily, enzyme can migrate to the nucleus upon activation Mammalia 5737
-
nucleus enzyme can migrate from the cytoplasm to the nucleus upon activation Mammalia 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates the enzyme, binds to beta- and gamma-phosphate of ATP and to Asp184 Mammalia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Mammalia the enzyme is a key player in cellular responses to the second messenger cAMP ADP + a phosphoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Mammalia
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation at Thr197 required for activation Mammalia

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate catalytic site structure, Asp166 is the catalytic base invariant in all kinases, activation mechanism, overview Mammalia
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein the enzyme is a key player in cellular responses to the second messenger cAMP Mammalia ADP + a phosphoprotein
-
 ?
ATP + a protein phosphorylates nuclear proteins important for gene replication Mammalia ADP + a phosphoprotein
-
 ?

Subunits

Subunits Comment Organism
More the enzyme possesses a regulatory and a catalytic subunit, 2 lobes are comprised in the core scaffold, the N-terminal beta-sheet and C-terminal alpha-helix lobe, which are joined by a polypeptide chain, the active is located at the interface of the 2 lobes Mammalia
tetramer alpha2beta2, the tetramer is inactive, binding of ATP causes release of the 2 catalytic subunits in active conformation Mammalia

Synonyms

Synonyms Comment Organism
cAPK
-
 Mammalia
cyclic AMP-dependent kinase
-
 Mammalia

Cofactor

Cofactor Comment Organism Structure
ATP binding site structure: spans both lobes, the N-terminal beta-sheet and C-terminal alpha-helix of the core scaffold, binding of ATP and release of ADP in the open enzyme conformation, residues Asp184, Lys72, and Asp166 are involved, schematic overview Mammalia