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Literature summary for 2.7.11.11 extracted from

  • Langer, T.; Sreeramulu, S.; Vogtherr, M.; Elshorst, B.; Betz, M.; Schieborr, U.; Saxena, K.; Schwalbe, H.
    Folding and activity of cAMP-dependent protein kinase mutants (2005), FEBS Lett., 579, 4049-4054.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cAMP dependent on Cricetulus griseus

Application

Application Comment Organism
drug development recombinant PKA can be mutated to resemble Akt/PKB, which cannot be easily recombinantly expressed, mutant PKA can be used as surrogate enzyme for Akt/PKB in drug design Cricetulus griseus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant PKAs in Escherichia coli strain BL21(DE3) Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
additional information removal of residues S10, S139, and S338 plus Akt/PKB-like mutations of the ATP binding site render PKA to a Akt/PKB similar enzyme, overview Cricetulus griseus
S10A/S139D/S338D site-directed mutagenesis, mutant shows activity and properties similar to the wild-type enzyme Cricetulus griseus
S10A/S139D/T197D/S338D site-directed mutagenesis, mutant shows reduced expression level in Escherichia coli and impaired folding Cricetulus griseus
S10A/S139D/T197E/S338D site-directed mutagenesis, mutant shows reduced expression level in Escherichia coli and impaired folding Cricetulus griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation at Ser10, Ser139, Thr197, and Ser338 Cricetulus griseus

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant PKAs from Escherichia coli strain BL21(DE3) Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Kemptide activity of catalytic PKA subunit Cricetulus griseus ADP + phosphorylated Kemptide
-
?
additional information the enzyme performs autophosphorylation at Ser10, Ser139, Thr197, and Ser338 Cricetulus griseus ?
-
?

Subunits

Subunits Comment Organism
More structure of catalytic subunit C with ATP binding cleft and activation loop, overview Cricetulus griseus

Synonyms

Synonyms Comment Organism
PKA
-
Cricetulus griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Cricetulus griseus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Cricetulus griseus