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Literature summary for 2.7.11.11 extracted from

  • Langer, T.; Vogtherr, M.; Elshorst, B.; Betz, M.; Schieborr, U.; Saxena, K.; Schwalbe, H.
    NMR backbone assignment of a protein kinase catalytic domain by a combination of several approaches: application to the catalytic subunit of cAMP-dependent protein kinase (2004), ChemBioChem, 5, 1508-1516.
    View publication on PubMed
Search for more literature for 2.7.11.11

Cloned(Commentary)

Cloned (Comment) Organism
subcloning and expression of His-tagged wild-type and mutant PKA catalytic subunits in Escherichia coli strain BL21(DE3), introduction of a tobacco etch virus cleavage site to remove the tag, expression of deuterated catalytic subunit in Escherichia coli strain CT19 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
S338A site-specific mutagenesis, mutation of a residue in the hydrophobic motif at the C-terminus of the catalytic subunit, catalytic activity is similar to the wild-type catalytic subunit, reduced protein stability compared to the wild-type enzyme Rattus norvegicus
T197A site-specific mutagenesis, mutation of activation loop residue of the catalytic subunit, inactive mutant, conformational changes and reduced protein stability compared to the wild-type enzyme Rattus norvegicus
T197A/S338A site-specific mutagenesis, highly unstable protein, tends to aggregation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
7-hydroxystaurosporine specific protein kinase inhibitor Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Subunits

Subunits Comment Organism
More catalytic subunit residue Ser338 stabilizes the enzyme Rattus norvegicus

Synonyms

Synonyms Comment Organism
PKA
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP optimal at 0.0025 mM, binding involves phosphorylation of Thr197 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0039
-
 7-hydroxystaurosporine recombinant wild-type catalytic subunit, pH 7.5 Rattus norvegicus
0.0049
-
 7-hydroxystaurosporine recombinant mutant S338A catalytic subunit, pH 7.5 Rattus norvegicus