Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
additional information
?
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O

beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the pyridine N and carboxyl groups are important for interaction with the enzyme
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
in the presence of Mg2+ and inorganic phosphate
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
+ whole cell lysates, pH 7.5, 37°C, 10 mM MgCl2, 2.5 mM dithiothreitol, 1 mM ATP, 25 microgram bovine serum albumin, with or without NAD+
termination by boiling for 60 seconds, analysis by thin layer chromatography (radiolabeled nicotinic acid) or ESI-MS, no NaMN detectable in Hep-G2 cells
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
essential for the NAD salvage pathway
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
mutants in the His-219 residue do catalyze the slow formation of nicotinic acid mononucleotide in the absence of ATP similarly to the wild type enzymes
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O

beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O

beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
5-phospho-alpha-D-ribose 1-diphosphate in complex with Mg
-
-
?
additional information

?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
?
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
?
additional information
?
-
nicotinamide is not substrate
-
-
?
additional information
?
-
-
nicotinamide is not substrate
-
-
?
additional information
?
-
quinolinic acid is not substrate
-
-
?
additional information
?
-
-
quinolinic acid is not substrate
-
-
?
additional information
?
-
-
enzyme is able to generate adenosine 5'-tetraphosphate using substrate ATP and product phosphate, reaction of EC 3.6.1.14. Accompanying a typically irreversible hydrolysis of ATP to ADP and phosphate, is a fully reversible phosphate transfer reaction between ATP and adenosine 5'-tetraphosphate
-
-
?
additional information
?
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
ATP and phosphoribose diphosphate compete for ATP-binding site
-
-
?
additional information
?
-
-
ATPase activity in the presence of either product and in the absence of phosphoribose diphosphate
-
-
?
additional information
?
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
negligible ATPase activity in the absence of nicotinic acid
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
additional information
?
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O

beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
essential for the NAD salvage pathway
-
-
r
additional information

?
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
molecular evolution mechanism
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxynicotinic acid
-
3-Pyridylcarbinol
-
weak inhibitor
3-Pyridylcarboxaldehyde
-
-
5-Fluoronicotinic acid
-
-
5-phospho-alpha-D-ribose 1-diphosphate
acetyl-CoA
at 1 mM 40% inhibition of activity (concentrations of substrates 1 mM)
CoA
at 1 mM 70% inhibition of activity (concentrations of substrates 1 mM)
D-fructose 1,6-bisphosphate
at 1 mM 36% inhibition of activity (concentrations of substrates 1 mM)
glutaryl-CoA
at 1 mM 42% inhibition of activity (concentrations of substrates 1 mM)
glyceraldehyde 3-phosphate
at 1 mM 27% inhibition of activity (concentrations of substrates 1 mM)
Methyl 3-pyridylcarbinol
-
weak inhibitor
MgADP-
-
non-competitive to Mg-ATP or Mg-diphosphate
Monoiodoacetic acid
-
1 mM and above
N-ethylmaleimide
-
0.1 mM and above
nicotinate mononucleotide
nucleoside diphosphates
-
e.g. ADP or GDP
phosphate
-
0.1 M and above
phosphoenolpyruvate
at 1 mM 33% inhibition of activity (concentrations of substrates 1 mM)
Pyrazine 2-carboxylic acid
-
-
Pyridazine-4,5-dicarboxylic acid
-
weak, 10% less activity
succinyl-CoA
at 1 mM 53% inhibition of activity (concentrations of substrates 1 mM)
Urea
-
competitive to nicotinate mononucleotide
5-phospho-alpha-D-ribose 1-diphosphate

-
at high concentrations
5-phospho-alpha-D-ribose 1-diphosphate
-
at high concentrations, when Mg2+ below 0.03 mM
ATP

at high substrate saturation
ATP
-
in the presence of Mn2+
ATP
-
at high concentrations with Mg2+ below 0.03 mM
ATP
-
3 mM, competitive inhibition of nicotinic acid mononucleotide formation in mutants enzymes
ATP
-
in the presence of Mn2+
Mg2+

-
at higher concentrations than ATP, when free Mg2+ ions occur
Mg2+
-
above 10 mM, significant pH alterations
NAD+

-
not
NADP+

-
not
Nicotinate analogues

-
-
-
Nicotinate analogues
-
not
-
nicotinate mononucleotide

-
not
nicotinate mononucleotide
-
competitive to both substrates
nicotinate mononucleotide
-
-
nicotinate mononucleotide
-
competitive to MgATP2-; non-competitive to nicotinate
nicotinate mononucleotide
-
competitive to nicotinate and noncompetitive to phosphoribobose diphosphate
additional information

-
no inhibition by nicotinamide; no inhibition by quinolinic acid, AMP, GMP
-
additional information
-
no inhibition by nicotinamide; no inhibition by nicotinate, up to 10 mM
-
additional information
-
no inhibition by 4-hydroxy-nicotinate
-
additional information
not inhibited by up to 1 mM NAD+, lack of feedback inhibition by NAD+
-
additional information
-
not inhibited by up to 1 mM NAD+, lack of feedback inhibition by NAD+
-
additional information
not inhibitory: FK866. NaPRTase acks a tunnel that, in nicotinamide phosphoribosiltransferase EC 2.4.2.12, represents the binding site of inhibitor FK866
-
additional information
-
not inhibitory: FK866. NaPRTase acks a tunnel that, in nicotinamide phosphoribosiltransferase EC 2.4.2.12, represents the binding site of inhibitor FK866
-
additional information
-
no inhibition by antibodies against pig kidney quinolinate phosphoribosyltransferase; no inhibition by nicotinamide mononucleotide, deamino-NAD+
-