Any feedback?
Literature summary for 6.3.4.21 extracted from
- A new paradigm for biochemical energy coupling. Salmonella typhimurium nicotinate phosphoribosyltransferase (1993), J. Biol. Chem., 268, 26004-26010.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of pncB gene on a multicopy pUC19 plasmid in Salmonella typhimurium | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
nicotinate | pncB gene, in the coupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 0.022 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pncB gene, in the coupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.29 | - |
nicotinate | pncB gene, in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pncB gene, in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
pncB gene, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bacterial suspension disruption and centrifugation, chromatography on Sephadex-G, DEAE-Toyopearl, Cibacron Blue and Sephacryl gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4ºC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
| t1/2: at least 2 months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAPRTase | - |
- |
| nicotinate phosphoribosyltransferase | - |
- |
| nicotinic acid phosphoribosyltransferase | - |
- |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed | Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
