EC Number |
General Stability |
Reference |
---|
6.3.4.21 | 50% activity recovered at salt concentrations of 50 mM, inactivation with salt concentrations below 20 mM, 20-35% glycerol partially protects |
637752 |
6.3.4.21 | dialysis against 50 mM Tris-HCl buffer at pH 7.3 in the absence of potassium phosphate, significant loss of activity |
637755 |
6.3.4.21 | first order activity loss in the presence of 1/200 trypsin/enzyme |
637759, 637760 |
6.3.4.21 | glycerol, 20-35%, stabilizes during dialysis against low buffer concentrations, more highly purified preparations require higher glycerol concentrations |
637752 |
6.3.4.21 | heat, unstable to, substrates protect |
637750 |
6.3.4.21 | rapidly inactivates the ATPase and nicotinic acid mononucleotide synthesis activities with cleavages at Arg-384 and Lys-374, ATP and 5-phospho-alpha-D-ribose 1-diphosphate increase the t1/2 for inactivation 8fold and 4fold, respectively, and 32fold, together |
637760 |
6.3.4.21 | sensitive to trypsin, partial proteolysis, 4fold increase in t1/2 for inactivation in the presence of 1 mM MgATP2- |
637759 |