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Literature summary for 6.3.4.21 extracted from

  • Niedel, J.; Dietrich, L.S.
    Nicotinate phosphoribosyltransferase of human erythrocytes. Purification and properties (1973), J. Biol. Chem., 248, 3500-3505.
    View publication on PubMed
Search for more literature for 6.3.4.21

General Stability

General Stability Organism
50% activity recovered at salt concentrations of 50 mM, inactivation with salt concentrations below 20 mM, 20-35% glycerol partially protects Homo sapiens
glycerol, 20-35%, stabilizes during dialysis against low buffer concentrations, more highly purified preparations require higher glycerol concentrations Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 nicotinate in the presence of ATP Homo sapiens
0.024
-
 nicotinate reduced to 0.0005 mM in the presence of ATP Homo sapiens
0.06 0.1 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens
0.1
-
 5-phospho-alpha-D-ribose 1-diphosphate 0.005 mM in the presence of ATP Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Homo sapiens first step in NAD+-(salvage) biosynthesis beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, chromatography on DEAE-cellulose, chromatography on Sephadex, affinity column chromatography and hydroxylapatite column chromatography, 30000fold purified Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.052
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, t1/2: 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information one mol ATP is cleaved per mol product formed Homo sapiens ?
-
 ?
additional information no ATPase activity in the absence of substrates Homo sapiens ?
-
 ?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Homo sapiens beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O first step in NAD+-(salvage) biosynthesis Homo sapiens beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
nicotinate phosphoribosyltransferase
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 broad, 0.2 M potassium phosphate buffer or 0.05 M Tris-Cl Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable, 0.2 M potassium phosphate buffer Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP activation Homo sapiens
ATP 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed Homo sapiens