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(2R)-tryptophanol + H2O
?
-
substrate for discovery of active mutants
-
-
?
(2S)-tryptophanol + H2O
?
-
substrate for discovery of active mutants
-
-
?
2-(1-benzothien-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-[(S)-1-(1,2,3,4-tetrahydro-benzo[4,5]thieno[2,3-c]pyridin-1-yl)methyl]-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-(4-fluoro-1H-indol-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-((S)-5-fluoro-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-(4-methyl-1H-indol-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-((S)-5-methyl-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-(5-fluoro-1H-indol-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-((S)-6-fluoro-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-(6-fluoro-1H-indol-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-((S)-7-fluoro-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-(7-fluoro-1H-indol-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-((S)-8-fluoro-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-(7-methyl-1H-indol-3-yl)ethanamine + secologanin
(4S,5R,6S)-4-((S)-8-methyl-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
2-benzofuran-3-yl-ethylamine + secologanin
(4S,5R,6S)-4-[(S)-1-(1,2,3,4-tetrahydro-benzo[4,5]furo[2,3-c]pyridin-1-yl)methyl]-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
3-(2-aminoethyl)-1H-indol-5-ol + secologanin
(4S,5R,6S)-4-((S)-6-hydroxy-2,3,4,9-tetrahydro-1H-b-carbolin-1-ylmethyl)-6-((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-5-vinyl-5,6-dihydro-4H-pyran-3-carboxylic acid methyl ester + H2O
-
-
-
-
?
3-(2-aminoethyl)-benzothiophene + secologanin
? + H2O
-
-
-
?
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
3-[(tert-butoxycarbonyl)amino]propyl (2S,3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + secologanin
? + H2O
competitive to strictosidine
-
-
?
4-fluorotryptamine + secologanin
4-fluoro-3-alpha(S)-strictosidine + H2O
-
-
-
?
4-methyltryptamine + secologanin
4-methyl-3-alpha(S)-strictosidine + H2O
-
-
-
?
4-methyltryptamine + secologanin
? + H2O
poor substrate
-
-
?
5,6-dihydroxytryptamine
?
-
-
-
?
5,6-dihydroxytryptamine + secologanin
? + H2O
-
-
-
?
5-bromotryptamine + H2O
?
-
substrate for discovery of active mutants
-
-
?
5-chlorotryptamine + H2O
?
-
substrate for discovery of active mutants
-
-
?
5-fluoro-tryptamine + H2O
?
-
-
-
-
?
5-fluorotryptamine
?
-
-
-
?
5-fluorotryptamine + secologanin
5-fluoro-3-alpha(S)-strictosidine + H2O
5-fluorotryptamine + secologanin
?
-
6% of the activity with trypamine
-
-
?
5-hydroxy-tryptamine + H2O
?
-
-
-
-
?
5-hydroxytryptamine + secologanin
?
-
4% of the activity with trypamine
-
-
?
5-hydroxytryptamine + secologanin
? + H2O
poor substrate
-
-
?
5-methoxy-tryptamine + H2O
?
-
-
-
-
?
5-methoxytryptamine + secologanin
10-methoxystrictosidine
Cinchona robusta
-
10% of activity
-
?
5-methoxytryptamine + secologanin
? + H2O
poor substrate
-
-
?
5-methyl-tryptamine + H2O
?
-
-
-
-
?
5-methyltryptamine + H2O
?
-
substrate for discovery of active mutants
-
-
?
6-fluoro-tryptamine + H2O
?
-
-
-
-
?
6-fluorotryptamine
?
-
-
-
?
6-fluorotryptamine + secologanin
6-fluoro-3-alpha(S)-strictosidine + H2O
6-hydroxytryptamine + secologanin
?
-
9% of the activity with trypamine
-
-
?
6-methoxy-tryptamine + H2O
?
-
-
-
-
?
6-methoxyltryptamine + secologanin
6-methoxy-3-alpha(S)-strictosidine + H2O
-
-
-
?
6-methoxytryptamine
?
-
-
-
?
6-methoxytryptamine + secologanin
? + H2O
poor substrate
-
-
?
6-methyl-tryptamine + H2O
?
-
-
-
-
?
6-methyltryptamine + secologanin
6-methyl-3-alpha(S)-strictosidine + H2O
7-azatryptamine + secologanin
3-alpha(S)-12-azastrictosidine
assay at pH 7.0, 28°C, reaction stopped by adding methanol
-
-
?
7-fluorotryptamine + secologanin
7-fluoro-3-alpha(S)-strictosidine + H2O
-
-
-
?
7-fluorotryptamine + secologanin
?
-
8% of the activity with trypamine
-
-
?
7-methyltryptamine
?
-
-
-
?
7-methyltryptamine + secologanin
7-methyl-3-alpha(S)-strictosidine + H2O
7-methyltryptamine + secologanin
?
-
15% of the activity with trypamine
-
-
?
7-methyltryptamine + secologanin
? + H2O
poor substrate
-
-
?
but-3-yn-1-yl (2S,3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + secologanin
? + H2O
competitive to strictosidine
-
-
?
D-tryptophan + H2O
?
-
substrate for discovery of active mutants
-
-
?
D-tryptophan methyl ester + H2O
?
-
substrate for discovery of active mutants
-
-
?
L-tryptophan + H2O
?
-
substrate for discovery of active mutants
-
-
?
methyl (2R,4S)-2-(2-methylpropoxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2R,4S)-2-(2-methylpropoxy)-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
cis- and trans-isomers are equally well accepted as substrates
-
-
?
methyl (2R,4S)-2-(cyclohexyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2R,4S)-2-(cyclohexyloxy)-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
significant preference for the trans-isomer
-
-
?
methyl (2R,4S)-2-ethoxy-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2R,4S)-2-ethoxy-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
cis- and trans-isomers are equally well accepted as substrates
-
-
?
methyl (2R,4S)-2-tert-butoxy-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2R,4S)-2-tert-butoxy-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
cis- and trans-isomers are equally well accepted as substrates
-
-
?
methyl (2S,4S)-2-(2-methylpropoxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2S,4S)-2-(2-methylpropoxy)-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
cis- and trans-isomers are equally well accepted as substrates
-
-
?
methyl (2S,4S)-2-ethoxy-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2S,4S)-2-ethoxy-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
cis- and trans-isomers are equally well accepted as substrates
-
-
?
methyl (2S,4S)-2-tert-butoxy-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + tryptamine
methyl (2S,4S)-2-tert-butoxy-4-[(1S)-2,3,4,9-tetrahydro-1H-beta-carbolin-1-ylmethyl]-3,4-dihydro-2H-pyran-5-carboxylate + H2O
cis- and trans-isomers are equally well accepted as substrates
-
-
?
N-omega-methyltryptamine + secologanin
dolichantoside + H2O
-
-
-
-
?
prop-2-yn-1-yl (2S,3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate + secologanin
? + H2O
competitive to strictosidine
-
-
?
secologanin + tryptamine
3-alpha(S)-strictosidine + H2O
-
-
-
?
serotonin + secologanin
5-hydroxy-3-alpha(S)-strictosidine + H2O
tryptamine + 2'-O-methylsecologanin
?
-
-
-
-
?
tryptamine + 2'-O-methylsecologanin
? + H2O
-
-
-
?
tryptamine + 3'-O-methylsecologanin
?
-
-
-
-
?
tryptamine + 3'-O-methylsecologanin
? + H2O
-
-
-
?
tryptamine + dihydrosecologanin
?
-
-
-
-
?
tryptamine + secologanic acid allyl ester
? + H2O
-
-
-
?
tryptamine + secologanic acid pentynyl ester
? + H2O
-
-
-
?
tryptamine + secologanic acid propynyl ester
? + H2O
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
3alpha(S)-strictosidine
tryptamine + secologanin
? + H2O
poor substrate
-
-
?
additional information
?
-
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
-
-
-
-
?
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
-
-
-
-
?
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
-
-
-
?
5-fluorotryptamine + secologanin
5-fluoro-3-alpha(S)-strictosidine + H2O
-
-
-
?
5-fluorotryptamine + secologanin
5-fluoro-3-alpha(S)-strictosidine + H2O
-
-
-
?
6-fluorotryptamine + secologanin
6-fluoro-3-alpha(S)-strictosidine + H2O
-
-
-
?
6-fluorotryptamine + secologanin
6-fluoro-3-alpha(S)-strictosidine + H2O
-
-
-
?
6-methyltryptamine + secologanin
6-methyl-3-alpha(S)-strictosidine + H2O
-
-
-
?
6-methyltryptamine + secologanin
6-methyl-3-alpha(S)-strictosidine + H2O
-
-
-
?
7-methyltryptamine + secologanin
7-methyl-3-alpha(S)-strictosidine + H2O
-
-
-
?
7-methyltryptamine + secologanin
7-methyl-3-alpha(S)-strictosidine + H2O
-
-
-
?
serotonin + secologanin
5-hydroxy-3-alpha(S)-strictosidine + H2O
-
-
-
?
serotonin + secologanin
5-hydroxy-3-alpha(S)-strictosidine + H2O
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Amsonia salicifolia
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Amsonia salicifolia
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Amsonia salicifolia
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Amsonia salicifolia
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Catharanthus pusillus
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Catharanthus pusillus
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
highly specific
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
the enzyme catalyses the biological Pictete-Spengler reaction of tryptamine and secologanin
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
higher activity in dark grown than in light grown cultures
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Cinchona robusta
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Cinchona robusta
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Ervatamia divaricatum
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Ervatamia divaricatum
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
the enzyme catalyses the biological Pictete-Spengler reaction of tryptamine and secologanin. Tryptamine is located at the bottom of the substrate binding pocket, where its primary amine group is connected with residue Glu309 by a hydrogen bond. The main amino acid residues involved in forming the active centre are Tyr105, Trp149, Val167, Met180, Val208, Phe226, Ser269, Met276, His277, His307, Phe308, Glu309, Leu323, and Phe324
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Stemmadenia tomentosa var. palmeri
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Stemmadenia tomentosa var. palmeri
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Stemmadenia tomentosa var. palmeri
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Stemmadenia tomentosa var. palmeri
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the strictosidine synthase plays a central role in the biosynthesis of all structural types of monoterpenoid indole alkaloids, overview
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
-
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
-
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the strictosidine synthase plays a central role in the biosynthesis of all structural types of monoterpenoid indole alkaloids, overview
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
assay at pH 7.0, 28°C, reaction stopped by adding methanol
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
-
the enzyme is as amine lyases and catalyzes a PictetSpengler-type condensation between tryptamine and the aldehyde secologanin which leads to the formation of (S)-strictosidine
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the strictosidine synthase catalyzes the condensation of tryptamine and secologanin to form strictosidine, which is the universal precursor for a wide range of pharmaceutical terpenoid indole alkaloids, TIA, e.g. in biosynthesis of reserpine and ajmalicine from the Chinese native TIAs-producing plant, overview
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the unique essential and conserved catalytic residue is Glu309
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
-
-
-
-
?
tryptamine + secologanin
3alpha(S)-strictosidine
-
-
-
-
?
tryptamine + secologanin
3alpha(S)-strictosidine
-
-
-
?
additional information
?
-
-
5-methoxytryptamine is not utilized
-
-
?
additional information
?
-
substrate specificity, e.g. with tryptamine derivatives, overview. No activity with 2-pyrrole-3-ethylamine, phenylethylamine, tyramine, homoveratrylamine, N-acetyl-5-hydroxytryptamine, N-methyltryptamine, 3-methylamine indole, 3-propylamine indole, 2-methyltryptamine, 2-ethyltryptamine, 5,6-dihydroxytryptamine, 5,7-dimethoxytryptamine, histamine, dopamine, 5-methyltryptamine, 6-methyltryptamine, and 5-methoxytryptamine, overview
-
-
?
additional information
?
-
-
tryptophan, phenylethylamine, tyramine and pyrrole cannot subsitute for tryptamine, overview on substituted tryptamines
-
-
?
additional information
?
-
substrate specificity, e.g. with tryptamine derivatives, overview. No activity with N-acetyl-5-hydroxytryptamine, N-methyltryptamine, N-omega-methyltryptamine, 2-methyl,5-hydroxytryptamine, 3-methylamine indole, 3-propylamine indole, 2-methyltryptamine, 2-ethyltryptamine, 5,7-dimethoxytryptamine, phenylalanine, histamine, dopamine, 5-methyltryptamine, 5-methoxytryptamine, 4,5-dimethyltryptamine, and 4-methyl-5-methoxytryptamine, and with secologanic acid, tarennoside, 18-n-butylsecologanin, and 18-tert-butylsecologanin, overview. Dihydrosecologanin and secologanin acid ethyl ester are poor substrates
-
-
?
additional information
?
-
-
substrate specificity, e.g. with tryptamine derivatives, overview. No activity with N-acetyl-5-hydroxytryptamine, N-methyltryptamine, N-omega-methyltryptamine, 2-methyl,5-hydroxytryptamine, 3-methylamine indole, 3-propylamine indole, 2-methyltryptamine, 2-ethyltryptamine, 5,7-dimethoxytryptamine, phenylalanine, histamine, dopamine, 5-methyltryptamine, 5-methoxytryptamine, 4,5-dimethyltryptamine, and 4-methyl-5-methoxytryptamine, and with secologanic acid, tarennoside, 18-n-butylsecologanin, and 18-tert-butylsecologanin, overview. Dihydrosecologanin and secologanin acid ethyl ester are poor substrates
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
tryptamine + secologanin
3-alpha(S)-strictosidine
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
-
-
-
-
?
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
-
-
-
-
?
3-alpha(S)-strictosidine + H2O
tryptamine + secologanin
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Amsonia salicifolia
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Amsonia salicifolia
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Catharanthus pusillus
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
higher activity in dark grown than in light grown cultures
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Cinchona robusta
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Ervatamia divaricatum
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Stemmadenia tomentosa var. palmeri
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
Stemmadenia tomentosa var. palmeri
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine
-
involved in synthesis of monoterpenoid indole alkaloides
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the strictosidine synthase plays a central role in the biosynthesis of all structural types of monoterpenoid indole alkaloids, overview
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the strictosidine synthase plays a central role in the biosynthesis of all structural types of monoterpenoid indole alkaloids, overview
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
-
the enzyme is as amine lyases and catalyzes a PictetSpengler-type condensation between tryptamine and the aldehyde secologanin which leads to the formation of (S)-strictosidine
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
the strictosidine synthase catalyzes the condensation of tryptamine and secologanin to form strictosidine, which is the universal precursor for a wide range of pharmaceutical terpenoid indole alkaloids, TIA, e.g. in biosynthesis of reserpine and ajmalicine from the Chinese native TIAs-producing plant, overview
-
-
?
tryptamine + secologanin
3-alpha(S)-strictosidine + H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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bovine serum albumin
-
1 mg/ml stimulates activity
-
additional information
-
gene SSL5 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL5 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL5 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL5 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL5 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
-
gene SSL6 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL6 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL6 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL6 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
gene SSL6 is induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus, overview
-
additional information
-
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, e.g. no induction by salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, e.g. no induction by salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, e.g. no induction by salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, e.g. no induction by salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, e.g. no induction by salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
-
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, while the other three AtSSL genes are induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, while the other three AtSSL genes are induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, while the other three AtSSL genes are induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, while the other three AtSSL genes are induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the AtSSL4 gene is constitutively expressed and not significantly induced by any treatment, while the other three AtSSL genes are induced to various degrees by plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, as well as by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
-
the gene SSL4 is constitutively expressed and not significantly induced by any treatment, e.g. with plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, or by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the gene SSL4 is constitutively expressed and not significantly induced by any treatment, e.g. with plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, or by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the gene SSL4 is constitutively expressed and not significantly induced by any treatment, e.g. with plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, or by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the gene SSL4 is constitutively expressed and not significantly induced by any treatment, e.g. with plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, or by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
additional information
the gene SSL4 is constitutively expressed and not significantly induced by any treatment, e.g. with plant defence signalling compounds, such as salicylic acid, methyl jasmonate and ethylene, or by wounding and exposure to the plant pathogens Alternaria brassicicola and cucumber mosaic virus
-
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Kutchan, T.M.; Bock, A.; Dittrich, H.
Heterologous expression of the plant proteins strictosidine synthase and berberine bridge enzyme in insect cell culture
Phytochemistry
35
353-360
1994
Rauvolfia serpentina
brenda
Treimer, J.F.; Zenk, M.H.
Purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation
Eur. J. Biochem.
101
225-233
1979
Amsonia salicifolia, Amsonia tabernaemontana, Catharanthus longifolius, Catharanthus pusillus, Catharanthus roseus, Catharanthus trichophyllus, Ervatamia divaricatum, Ochrosia elliptica, Rauvolfia verticillata, Rauvolfia vomitoria, Amsonia orientalis, Stemmadenia tomentosa var. palmeri, Vinca major, Vinca minor, Voacanga africana
brenda
Mizukami, H.; Nordlov, H.; Lee, S.L.; Scott, A.I.
Purification and properties of strictosidine synthetase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cell
Biochemistry
18
3760-3763
1979
Catharanthus roseus
brenda
Treimer, J.F.; Zenk, M.H.
Strictosidine synthase from cell cultrues of Apocynaceae plants
FEBS Lett.
97
159-162
1979
Amsonia salicifolia, Catharanthus roseus, Ochrosia elliptica, Rauvolfia vomitoria, Amsonia orientalis, Stemmadenia tomentosa var. palmeri, Vinca major, Vinca minor, Voacanga africana
-
brenda
Pfitzner, U.; Zenk, M.H.
Immobilization of strictosidine synthase from Catharanthus cell cultures and preparative synthesis of strictosidine
Planta Med.
46
10-14
1982
Catharanthus roseus
brenda
Skinner, S.E.; Walton, N.J.; Robins, R.J.; Rhodes, M.J.C.
Tryptophan decarboxylase, strictosidine synthase and alkaloid production by Cinchona ledgerinana suspension cultures
Phytochemistry
26
721-725
1987
Cinchona calisaya
-
brenda
Pfitzner, U.; Zenk, M.H.
Isoation and immobilization of strictosidine synthase
Methods Enzymol.
136
342-350
1987
Catharanthus roseus
-
brenda
Hampp, N.; Zenk, M.H.
Homogeneous strictosidine synthase from cell suspension cultures of Rauvolfia serpentina
Phytochemistry
27
3811-3815
1988
Rauvolfia serpentina
-
brenda
Pennings, E.J.M.; Van den Bosch, R.A.; Van der Heijden, R.; Stevens, L.H.; Duine, J.A.; Verpoorte, R.
Assay of trictosidine synthase from plant cell cultures by high-performance liquid chromatography
Anal. Biochem.
176
412-415
1989
Catharanthus roseus, Tabernaemontana orientalis
brenda
Roessner, C.A.; Devagupta, R.; Hasan, M.; Williams, H.J.; Scott, A.I.
Purification of an indole alkaloid biosynthetic enzyme, strictosidine synthase, from a recombinant strain of Escherichia coli
Protein Expr. Purif.
3
295-300
1992
Catharanthus roseus
brenda
Bracher, D.; Kutchan, T.M.
Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis
Arch. Biochem. Biophys.
294
717-723
1992
Rauvolfia mannii, Rauvolfia serpentina
brenda
Stevens, L.H.; Giroud, C.; Pennings, E.J.M.; Verpoorte, R.
Purification and characterization of strictosidine synthase from a suspension culture of Cinchona robusta
Phytochemistry
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1993
Catharanthus roseus, Cinchona robusta
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brenda
de Waal, A.; Meijer, A.H.; Verpoorte, R.
Strictosidine synthase from Catharanthus roseus: purification and characterization of multiple forms
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Catharanthus roseus
brenda
Hallard, D.; Van der Heijden, R.; Verpoorte, R.; Lopes Cardoso, M.I.; Pasquali, G.; Memelink, J.; Hoge, J.H.C.
Suspension cultured transgenic cells of Nicotiana tabacum expressing tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus produce strictosidine upon secologanin feeding.
Plant Cell Rep.
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Catharanthus roseus
brenda
Canel, C.; Lopes-Cardoso, M.I.; Whitmer, S.; van der Fits, L.; Pasquali, G.; van der Heijden, R.; Hoge, J.H.; Verpoorte, R.
Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus
Planta
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1998
Catharanthus roseus (P18417), Catharanthus roseus
brenda
Pasquali, G.; Erven, A.S.; Ouwerkerk, P.B.; Menke, F.L.; Memelink, J.
The promoter of the strictosidine synthase gene from periwinkle confers elicitor-inducible expression in transgenic tobacco and binds nuclear factors GT-1 and GBF
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Catharanthus roseus
brenda
Whitmer, S.; van der Heijden, R.; Verpoorte, R.
Effect of precursor feeding on alkaloid accumulation by a strictosidine synthase over-expressing transgenic cell line S1 of Catharanthus roseus
Plant Cell Tissue Organ Cult.
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2002
Catharanthus roseus
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brenda
Yamazaki, Y.; Urano, A.; Sudo, H.; Kitajima, M.; Takayama, H.; Yamazaki, M.; Aimi, N.; Saito, K.
Metabolite profiling of alkaloids and strictosidine synthase activity in camptothecin producing plants
Phytochemistry
62
461-470
2003
Ophiorrhiza pumila
brenda
Koepke, J.; Ma, X.; Fritzsch, G.; Michel, H.; Stockigt, J.
Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine
Acta Crystallogr. Sect. D
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690-693
2005
Rauvolfia serpentina
brenda
Ma, X.; Koepke, J.; Fritzsch, G.; Diem, R.; Kutchan, T.M.; Michel, H.; Stockigt, J.
Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family
Biochim. Biophys. Acta
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2004
Rauvolfia serpentina
brenda
McCoy, E.; Galan, M.C.; O'Connor S.E.
Substrate specificity of strictosidine synthase
Bioorg. Med. Chem. Lett.
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2475-2478
2006
Escherichia coli
brenda
Ma, X.; Panjikar, S.; Koepke, J.; Loris, E.; Stockigt, J.
The Structure of Rauvolfia serpentina Strictosidine Synthase Is a Novel Six-Bladed {beta}-Propeller Fold in Plant Proteins
Plant Cell
18
907-920
2006
Rauvolfia serpentina (P68175), Rauvolfia serpentina
brenda
Chen, S.; Galan, M.C.; Coltharp, C.; OConnor, S.E.
Redesign of a central enzyme in alkaloid biosynthesis
Chem. Biol.
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Catharanthus roseus
brenda
Bernhardt, P.; McCoy, E.; OConnor, S.E.
Rapid identification of enzyme variants for reengineered alkaloid biosynthesis in periwinkle
Chem. Biol.
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2007
Catharanthus roseus
brenda
Loris, E.A.; Panjikar, S.; Ruppert, M.; Barleben, L.; Unger, M.; Schuebel, H.; Stoeckigt, J.
Structure-based engineering of strictosidine synthase: auxiliary for alkaloid libraries
Chem. Biol.
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979-985
2007
Rauvolfia serpentina
brenda
Maresh, J.J.; Giddings, L.A.; Friedrich, A.; Loris, E.A.; Panjikar, S.; Trout, B.L.; Stoeckigt, J.; Peters, B.; OConnor, S.E.
Strictosidine synthase: mechanism of a Pictet-Spengler catalyzing enzyme
J. Am. Chem. Soc.
130
710-723
2008
Catharanthus roseus, Rauvolfia serpentina (P68175)
brenda
Sohani, M.M.; Schenk, P.M.; Schultz, C.J.; Schmidt, O.
Phylogenetic and transcriptional analysis of a strictosidine synthase-like gene family in Arabidopsis thaliana reveals involvement in plant defence responses
Plant Biol.
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105-117
2009
Arabidopsis thaliana, Arabidopsis thaliana (Q9CAZ7), Arabidopsis thaliana (Q9SD04), Arabidopsis thaliana (Q9SD05), Arabidopsis thaliana (Q9SD07)
brenda
Stoeckigt, J.; Barleben, L.; Panjikar, S.; Loris, E.A.
3D-Structure and function of strictosidine synthase - the key enzyme of monoterpenoid indole alkaloid biosynthesis
Plant Physiol. Biochem.
46
340-355
2008
Catharanthus roseus (P18417), Rauvolfia serpentina (P68175), Rauvolfia serpentina
brenda
Chen, R.; Liao, Z.H.; Chen, M.; Wang, Q.; Yang, C.X.; Yang, Y.J.
Molecular cloning and characterization of the strictosidine synthase gene from Rauwolfia verticillata
Russ. J. Plant Physiol.
55
670-675
2008
Rauvolfia verticillata (Q1L6F4)
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brenda
Yang, L.; Zou, H.; Zhu, H.; Ruppert, M.; Gong, J.; Stoeckigt, J.
Improved expression of His(6)-tagged strictosidine synthase cDNA for chemo-enzymatic alkaloid diversification
Chem. Biodivers.
7
860-870
2010
Rauvolfia serpentina (P68175)
brenda
Lu, Y.; Wang, H.; Wang, W.; Qian, Z.; Li, L.; Wang, J.; Zhou, G.; Kai, G.
Molecular characterization and expression analysis of a new cDNA encoding strictosidine synthase from Ophiorrhiza japonica
Mol. Biol. Rep.
36
1845-1852
2009
Ophiorrhiza japonica
brenda
Stockigt, J.; Hammes, B.; Ruppert, M.
Construction and expression of a dual vector for chemo-enzymatic synthesis of plant indole alkaloids in Escherichia coli
Nat. Prod. Res.
24
759-766
2010
Rauvolfia serpentina
brenda
Bernhardt, P.; OConnor, S.E.
Synthesis and biochemical evaluation of des-vinyl secologanin aglycones with alternate stereochemistry
Tetrahedron Lett.
50
7118-7120
2009
Rauvolfia serpentina (P68175)
brenda
Guirimand, G.; Courdavault, V.; Lanoue, A.; Mahroug, S.; Guihur, A.; Blanc, N.; Giglioli-Guivarch, N.; St-Pierre, B.; Burlat, V.
Strictosidine activation in Apocynaceae: Towards a nuclear time bomb?
BMC Plant Biol.
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182
2010
Catharanthus roseus
brenda
Kusari, S.; Zuehlke, S.; Spiteller, M.
Effect of artificial reconstitution of the interaction between the plant Camptotheca acuminata and the fungal endophyte Fusarium solani on camptothecin biosynthesis
J. Nat. Prod.
74
764-775
2011
Camptotheca acuminata
brenda
Yamazaki, M.; Asano, T.; Yamazaki, Y.; Sirikantaramas, S.; Sudo, H.; Saito, K.
Biosynthetic system of camptothecin: An anticancer plant product
Pure Appl. Chem.
82
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2010
Ophiorrhiza pumila (Q94LW9)
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brenda
Fischereder, E.; Pressnitz, D.; Kroutil, W.; Lutz, S.
Engineering strictosidine synthase: rational design of a small, focused circular permutation library of the beta-propeller fold enzyme
Bioorg. Med. Chem.
22
5633-5637
2014
Rauvolfia serpentina
brenda
Vera-Reyes, I.; Huerta-Heredia, A.A.; Ponce-Noyola, T.; Flores-Sanchez, I.J.; Esparza-Garcia, F.; Cerda-Garcia-Rojas, C.M.; Trejo-Tapia, G.; Ramos-Valdivia, A.C.
Strictosidine-related enzymes involved in the alkaloid biosynthesis of Uncaria tomentosa root cultures grown under oxidative stress
Biotechnol. Prog.
29
621-630
2013
Uncaria tomentosa
brenda
Verma, P.; Sharma, A.; Khan, S.A.; Shanker, K.; Mathur, A.K.
Over-expression of Catharanthus roseus tryptophan decarboxylase and strictosidine synthase in rol gene integrated transgenic cell suspensions of Vinca minor
Protoplasma
252
373-381
2015
Catharanthus roseus (P18417), Catharanthus roseus
brenda
Pandey, S.S.; Singh, S.; Babu, C.S.; Shanker, K.; Srivastava, N.K.; Shukla, A.K.; Kalra, A.
Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis
Sci. Rep.
6
26583
2016
Catharanthus roseus (P18417), Catharanthus roseus
brenda
Cui, L.; Ni, X.; Ji, Q.; Teng, X.; Yang, Y.; Wu, C.; Zekria, D.; Zhang, D.; Kai, G.
Co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila
Sci. Rep.
5
8227
2015
Catharanthus roseus (P18417), Catharanthus roseus
brenda
Zhu, H.; Kercmar, P.; Wu, F.; Rajendran, C.; Sun, L.; Wang, M.; Stoeckigt, J.
Using strictosidine synthase to prepare novel alkaloids
Curr. Med. Chem.
22
1880-1888
2015
Rauvolfia serpentina (P68175)
brenda
Sharma, A.; Verma, P.; Mathur, A.; Mathur, A.K.
Overexpression of tryptophan decarboxylase and strictosidine synthase enhanced terpenoid indole alkaloid pathway activity and antineoplastic vinblastine biosynthesis in Catharanthus roseus
Protoplasma
255
1281-1294
2018
Catharanthus roseus (P18417), Catharanthus roseus
brenda
Zou, T.; Li, S.; Liu, M.; Wang, T.; Xiao, Q.; Chen, D.; Li, Q.; Liang, Y.; Zhu, J.; Liang, Y.; Deng, Q.; Wang, S.; Zheng, A.; Wang, L.; Li, P.
An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
Sci. Rep.
7
6863
2017
Oryza sativa (Q84Q89), Oryza sativa
brenda
Soltani, N.; Nazarian-Firouzabadi, F.; Shafeinia, A.; Sadr, A.S.; Shirali, M.
The expression of terpenoid indole alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment
Mol. Biol. Rep.
47
7009-7016
2020
Catharanthus roseus (P18417), Catharanthus roseus
brenda