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Literature summary for 4.3.3.2 extracted from
- Purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation (1979), Eur. J. Biochem., 101, 225-233.
General Stability
| General Stability | Organism |
|---|---|
| high loss of activity on repeated freezing and thawing | Catharanthus roseus |
| stable on repeated freezing and thawing | Catharanthus roseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition with secologanin | Catharanthus roseus | |
| p-chloromercuribenzoate | - |
Amsonia orientalis |  |
| p-chloromercuribenzoate | - |
Amsonia salicifolia | |
| p-chloromercuribenzoate | - |
Catharanthus roseus | |
| p-chloromercuribenzoate | - |
Ochrosia elliptica | |
| p-chloromercuribenzoate | - |
Rauvolfia vomitoria | |
| p-chloromercuribenzoate | - |
Stemmadenia tomentosa var. palmeri | |
| p-chloromercuribenzoate | - |
Vinca major | |
| p-chloromercuribenzoate | - |
Voacanga africana | |
| tryptamine | inhibitory above 1.2 mM | Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
tryptamine | pH 7.0, 35°C | Catharanthus roseus | |
| 3.4 | - |
secologanin | pH 7.0, 35°C | Catharanthus roseus | |
| 3.5 | - |
dihydrosecologanin | pH 7.0, 35°C | Catharanthus roseus | |
| 8 | - |
3'-O-methylsecologanin | pH 7.0, 35°C | Catharanthus roseus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Catharanthus roseus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tryptamine + secologanin | Catharanthus roseus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Amsonia salicifolia | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Catharanthus pusillus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Catharanthus trichophyllus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Rauvolfia vomitoria | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Amsonia orientalis | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Vinca major | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Rauvolfia verticillata | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Vinca minor | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Amsonia tabernaemontana | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Catharanthus longifolius | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Ervatamia divaricatum | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Ochrosia elliptica | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Stemmadenia tomentosa var. palmeri | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | Voacanga africana | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amsonia orientalis | - |
- |
- |
| Amsonia salicifolia | - |
- |
- |
| Amsonia tabernaemontana | - |
- |
- |
| Catharanthus longifolius | - |
- |
- |
| Catharanthus pusillus | - |
- |
- |
| Catharanthus roseus | - |
i.e. Vinca rosea | - |
| Catharanthus trichophyllus | - |
- |
- |
| Ervatamia divaricatum | - |
- |
- |
| Ochrosia elliptica | - |
- |
- |
| Rauvolfia verticillata | - |
- |
- |
| Rauvolfia vomitoria | - |
- |
- |
| Stemmadenia tomentosa var. palmeri | - |
- |
- |
| Vinca major | - |
- |
- |
| Vinca minor | - |
- |
- |
| Voacanga africana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Catharanthus roseus | - |
| cell culture | - |
Amsonia salicifolia | - |
| cell culture | - |
Rauvolfia vomitoria | - |
| cell culture | - |
Amsonia orientalis | - |
| cell culture | - |
Vinca major | - |
| cell culture | - |
Ochrosia elliptica | - |
| cell culture | - |
Stemmadenia tomentosa var. palmeri | - |
| cell culture | - |
Voacanga africana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.07 | - |
purified enzyme, pH 7.0, 35°C | Catharanthus roseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°, 1 year, no loss of activity | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorotryptamine + secologanin | 6% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
| 5-hydroxytryptamine + secologanin | 4% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
| 6-hydroxytryptamine + secologanin | 9% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
| 7-fluorotryptamine + secologanin | 8% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
| 7-methyltryptamine + secologanin | 15% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
| tryptamine + 2'-O-methylsecologanin | - |
Catharanthus roseus | ? | - |
? | |
| tryptamine + 3'-O-methylsecologanin | - |
Catharanthus roseus | ? | - |
? | |
| tryptamine + dihydrosecologanin | - |
Catharanthus roseus | ? | - |
? | |
| tryptamine + secologanin | - |
Amsonia salicifolia | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Catharanthus pusillus | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Catharanthus trichophyllus | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Rauvolfia vomitoria | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Amsonia orientalis | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Vinca major | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Rauvolfia verticillata | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Vinca minor | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Amsonia tabernaemontana | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Catharanthus longifolius | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Ervatamia divaricatum | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Ochrosia elliptica | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Stemmadenia tomentosa var. palmeri | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | - |
Voacanga africana | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | highly specific | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Amsonia salicifolia | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus pusillus | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus trichophyllus | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Rauvolfia vomitoria | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Amsonia orientalis | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Vinca major | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Rauvolfia verticillata | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Vinca minor | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Amsonia tabernaemontana | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus longifolius | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Ervatamia divaricatum | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Ochrosia elliptica | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Stemmadenia tomentosa var. palmeri | 3-alpha(S)-strictosidine | - |
? | |
| tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Voacanga africana | 3-alpha(S)-strictosidine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Catharanthus roseus |
| 45 | - |
- |
Amsonia salicifolia |
| 45 | - |
- |
Catharanthus pusillus |
| 45 | - |
- |
Catharanthus trichophyllus |
| 45 | - |
- |
Vinca major |
| 45 | - |
- |
Rauvolfia verticillata |
| 45 | - |
- |
Vinca minor |
| 45 | - |
- |
Amsonia tabernaemontana |
| 45 | - |
- |
Catharanthus longifolius |
| 45 | - |
- |
Ervatamia divaricatum |
| 45 | - |
- |
Ochrosia elliptica |
| 45 | - |
- |
Stemmadenia tomentosa var. palmeri |
| 50 | - |
- |
Rauvolfia vomitoria |
| 50 | - |
- |
Amsonia orientalis |
| 50 | - |
- |
Voacanga africana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Catharanthus roseus |
| 6 | - |
- |
Amsonia orientalis |
| 6.8 | - |
- |
Catharanthus roseus |
| 6.9 | - |
- |
Catharanthus pusillus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Catharanthus roseus |
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