Literature summary for 4.3.3.2 extracted from

Koepke, J.; Ma, X.; Fritzsch, G.; Michel, H.; Stockigt, J.
Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine (2005), Acta Crystallogr. Sect. D, 61, 690-693.

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Application

Application	Comment	Organism
medicine	anti-arrhythmic ajmaline agents	Rauvolfia serpentina
synthesis	involved in the biosynthesis of almost all plant monoterpenoid indole alkaloids	Rauvolfia serpentina

Cloned(Commentary)

Cloned (Comment)	Organism
cloned with His-tag, expression in Escherichia coli strain M15, after expression and Ni-NTA column removed by DAPase	Rauvolfia serpentina

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of free STR1 and co-crystallization of STR1 and tryptamine, 2.95 A and 2.38 A under cryoconditions, crystal growing by hanging drop vapor diffusion, 302-304 K, 0.8 M potassium sodium tartrate tetrahydrate, pH 7.5 - 8.0, multiple wavelength anomalous dispersion method	Rauvolfia serpentina

Organism

Organism	UniProt	Comment	Textmining
Rauvolfia serpentina	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column, Mono Q column, concentration to 10 mg/ml in 10 mM Tris-HCl pH 8.0 before crystallization	Rauvolfia serpentina

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tryptamine + secologanin	-	Rauvolfia serpentina	3alpha(S)-strictosidine	-	?

Synonyms

Synonyms	Comment	Organism
STR1	-	Rauvolfia serpentina

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Release 2023.1 (January 2023)