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Literature summary for 4.3.3.2 extracted from

  • Pfitzner, U.; Zenk, M.H.
    Immobilization of strictosidine synthase from Catharanthus cell cultures and preparative synthesis of strictosidine (1982), Planta Med., 46, 10-14.
    View publication on PubMed

Application

Application Comment Organism
synthesis immobilized enzyme can be used to synthesize large quantities of 3-alpha(S)-strictosidine, that is a common intermediate in the biosynthesis of indole alkaloids Catharanthus roseus

General Stability

General Stability Organism
immobilized enzyme is more stable to changes in pH and temperature than soluble enzyme Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
tryptamine inhibitory above 1 mM, soluble and immobilized enzyme Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
tryptamine immobilized enzyme, pH 6.5, 37°C Catharanthus roseus
2.1
-
secologanin immobilized enzyme, pH 6.5, 37°C Catharanthus roseus
2.3
-
tryptamine soluble enzyme, pH 6.5, 37°C Catharanthus roseus
3.4
-
secologanin soluble enzyme, pH 6.5, 37°C Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tryptamine + secologanin Catharanthus roseus involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Catharanthus roseus
-

Storage Stability

Storage Stability Organism
4°C, immobilized enzyme, 2 years, 55% loss of activity Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tryptamine + secologanin
-
Catharanthus roseus 3-alpha(S)-strictosidine
-
?
tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Catharanthus roseus 3-alpha(S)-strictosidine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
immobilized enzyme, half life: 68 d, 6% of initial activity after one year Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
immobilized enzyme Catharanthus roseus
6.8
-
soluble enzyme Catharanthus roseus